Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against <i>Botryris cinerea</i>
This study mainly evaluated the broad-spectrum fungicidal activity of ε-poly L lysine (ε-PL) against 12 pathogenic fungi. We further demonstrated synergistic antifungal activity of ε-PL combined with wuyiencin against <i>Botryris cinerea</i>. The combined bio-fungicide achieved an inhibi...
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MDPI AG
2022-05-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/5/971 |
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| author | Zhaoyang Lv Yanxuan Lu Boya Li Liming Shi Kecheng Zhang Beibei Ge |
| author_facet | Zhaoyang Lv Yanxuan Lu Boya Li Liming Shi Kecheng Zhang Beibei Ge |
| author_sort | Zhaoyang Lv |
| collection | DOAJ |
| description | This study mainly evaluated the broad-spectrum fungicidal activity of ε-poly L lysine (ε-PL) against 12 pathogenic fungi. We further demonstrated synergistic antifungal activity of ε-PL combined with wuyiencin against <i>Botryris cinerea</i>. The combined bio-fungicide achieved an inhibition rate of 100% for mycelial growth using ε-PL at 500 μg/mL + wuyiencin at 50 μg/mL and for spore germination using ε-PL at 200 μg/mL + wuyiencin at 80 μg/mL in vitro. This synergistic spore and mycelia-damaging effect of the combination was confirmed using scanning electron microscopy. In vivo assays with combined bio-fungicide (1500 μg/mL ε-PL + 60 μg/mL wuyiencin) on detached leaves showed depressed growth and development of the spores of <i>B. cinerea</i>. The synergistic effect was further tested in combinations of ε-PL with wuyiencin by measuring the fractional inhibition concentration index (FICI) value below 0.5. Moreover, ε-PL and wuyiencin inoculation before <i>B. cinerea</i> infection significantly increased the superoxide dismutase, peroxidase, catalase, and phenylalanine ammonia-lyase activities, which suggested their involvement in tomato defense responses to disease to minimize damage to <i>B. cinerea.</i> These findings revealed that a combined bio-fungicide comprising ε-PL and wuyiencin had a good prospect for controlling plant fungal disease. |
| first_indexed | 2024-03-10T03:23:06Z |
| format | Article |
| id | doaj.art-6c728c63d2e143f29e990b3db20bcf6a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T03:23:06Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-6c728c63d2e143f29e990b3db20bcf6a2023-11-23T12:15:52ZengMDPI AGMicroorganisms2076-26072022-05-0110597110.3390/microorganisms10050971Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against <i>Botryris cinerea</i>Zhaoyang Lv0Yanxuan Lu1Boya Li2Liming Shi3Kecheng Zhang4Beibei Ge5Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, No. 2, Haidian District, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, No. 2, Haidian District, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, No. 2, Haidian District, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, No. 2, Haidian District, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, No. 2, Haidian District, Beijing 100193, ChinaInstitute of Plant Protection, Chinese Academy of Agricultural Sciences, Yuanmingyuan West Road, No. 2, Haidian District, Beijing 100193, ChinaThis study mainly evaluated the broad-spectrum fungicidal activity of ε-poly L lysine (ε-PL) against 12 pathogenic fungi. We further demonstrated synergistic antifungal activity of ε-PL combined with wuyiencin against <i>Botryris cinerea</i>. The combined bio-fungicide achieved an inhibition rate of 100% for mycelial growth using ε-PL at 500 μg/mL + wuyiencin at 50 μg/mL and for spore germination using ε-PL at 200 μg/mL + wuyiencin at 80 μg/mL in vitro. This synergistic spore and mycelia-damaging effect of the combination was confirmed using scanning electron microscopy. In vivo assays with combined bio-fungicide (1500 μg/mL ε-PL + 60 μg/mL wuyiencin) on detached leaves showed depressed growth and development of the spores of <i>B. cinerea</i>. The synergistic effect was further tested in combinations of ε-PL with wuyiencin by measuring the fractional inhibition concentration index (FICI) value below 0.5. Moreover, ε-PL and wuyiencin inoculation before <i>B. cinerea</i> infection significantly increased the superoxide dismutase, peroxidase, catalase, and phenylalanine ammonia-lyase activities, which suggested their involvement in tomato defense responses to disease to minimize damage to <i>B. cinerea.</i> These findings revealed that a combined bio-fungicide comprising ε-PL and wuyiencin had a good prospect for controlling plant fungal disease.https://www.mdpi.com/2076-2607/10/5/971ε-PLwuyiencinfungicidemycelial growthinduced resistance |
| spellingShingle | Zhaoyang Lv Yanxuan Lu Boya Li Liming Shi Kecheng Zhang Beibei Ge Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against <i>Botryris cinerea</i> Microorganisms ε-PL wuyiencin fungicide mycelial growth induced resistance |
| title | Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against <i>Botryris cinerea</i> |
| title_full | Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against <i>Botryris cinerea</i> |
| title_fullStr | Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against <i>Botryris cinerea</i> |
| title_full_unstemmed | Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against <i>Botryris cinerea</i> |
| title_short | Effects of ε-Poly-L-Lysine Combined with Wuyiencin as a Bio-Fungicide against <i>Botryris cinerea</i> |
| title_sort | effects of ε poly l lysine combined with wuyiencin as a bio fungicide against i botryris cinerea i |
| topic | ε-PL wuyiencin fungicide mycelial growth induced resistance |
| url | https://www.mdpi.com/2076-2607/10/5/971 |
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