Effects of Different Basal Cell Culture Media upon the Osteogenic Response of hMSCs Evaluated by <sup>99m</sup>Tc-HDP Labeling
The osteogenic differentiation of mesenchymal stem cells is now a standard procedure in modern bone tissue engineering. As this is a promising field for future clinical applications, many cell culture media exist to promote osteogenic differentiation. Prior to differentiation, cells must be expanded...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/11/6288 |
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| author | Tobias Grossner Uwe Haberkorn Jakob Hofmann Tobias Gotterbarm |
| author_facet | Tobias Grossner Uwe Haberkorn Jakob Hofmann Tobias Gotterbarm |
| author_sort | Tobias Grossner |
| collection | DOAJ |
| description | The osteogenic differentiation of mesenchymal stem cells is now a standard procedure in modern bone tissue engineering. As this is a promising field for future clinical applications, many cell culture media exist to promote osteogenic differentiation. Prior to differentiation, cells must be expanded to obtain sufficient numbers for experiments. Little evidence is available regarding the optimal media combination for expansion and differentiation to maximize the osteogenic response. Therefore, human BM-MSCs (<i>n</i> = 6) were expanded in parallel in DMEM (Dulbecco’s Modified Eagle Medium) LG (Low Glucose) and α-MEM (Minimum Essential Media alpha-modification), followed by simultaneous monolayer differentiation toward the osteogenic lineage in: 1. DMEM LG (Low Glucose), 2. DMEM HG (High Glucose), 3. α-MEM, 4. “Bernese medium”, and 5. “Verfaillie medium”, with a corresponding negative control (total 20 groups). As a marker for osteogenic differentiation, hydroxyapatite was accessed using radioactive <sup>99m</sup>Tc-HDP labeling and quantitative alizarin red staining. The results indicate that all media except “Bernese medium” are suitable for osteogenic differentiation, while there was evidence that DMEM LG is partly superior when used for expansion and differentiation of BM-hMSCs. Using “Verfaillie medium” after DMEM LG expansion led to the highest grade of osteogenic differentiation. Nevertheless, the difference was not significant. Therefore, we recommend using DMEM LG for robust osteogenic differentiation, as it is highly suitable for that purpose, economical compared to other media, and requires little preparation time. |
| first_indexed | 2024-03-10T01:14:10Z |
| format | Article |
| id | doaj.art-6c731e12f739406d80fc3f658790ea0e |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:14:10Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6c731e12f739406d80fc3f658790ea0e2023-11-23T14:12:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-06-012311628810.3390/ijms23116288Effects of Different Basal Cell Culture Media upon the Osteogenic Response of hMSCs Evaluated by <sup>99m</sup>Tc-HDP LabelingTobias Grossner0Uwe Haberkorn1Jakob Hofmann2Tobias Gotterbarm3Clinic for Orthopedics and Trauma Surgery, Center for Orthopedics, Trauma Surgery and Paraplegiology, University Hospital Heidelberg, 69120 Heidelberg, GermanyDepartment of Nuclear Medicine, University Hospital Heidelberg, 69120 Heidelberg, GermanyClinic for Orthopedics and Trauma Surgery, Center for Orthopedics, Trauma Surgery and Paraplegiology, University Hospital Heidelberg, 69120 Heidelberg, GermanyDepartment of Orthopedics and Traumatology, Kepler University Hospital, 4020 Linz, AustriaThe osteogenic differentiation of mesenchymal stem cells is now a standard procedure in modern bone tissue engineering. As this is a promising field for future clinical applications, many cell culture media exist to promote osteogenic differentiation. Prior to differentiation, cells must be expanded to obtain sufficient numbers for experiments. Little evidence is available regarding the optimal media combination for expansion and differentiation to maximize the osteogenic response. Therefore, human BM-MSCs (<i>n</i> = 6) were expanded in parallel in DMEM (Dulbecco’s Modified Eagle Medium) LG (Low Glucose) and α-MEM (Minimum Essential Media alpha-modification), followed by simultaneous monolayer differentiation toward the osteogenic lineage in: 1. DMEM LG (Low Glucose), 2. DMEM HG (High Glucose), 3. α-MEM, 4. “Bernese medium”, and 5. “Verfaillie medium”, with a corresponding negative control (total 20 groups). As a marker for osteogenic differentiation, hydroxyapatite was accessed using radioactive <sup>99m</sup>Tc-HDP labeling and quantitative alizarin red staining. The results indicate that all media except “Bernese medium” are suitable for osteogenic differentiation, while there was evidence that DMEM LG is partly superior when used for expansion and differentiation of BM-hMSCs. Using “Verfaillie medium” after DMEM LG expansion led to the highest grade of osteogenic differentiation. Nevertheless, the difference was not significant. Therefore, we recommend using DMEM LG for robust osteogenic differentiation, as it is highly suitable for that purpose, economical compared to other media, and requires little preparation time.https://www.mdpi.com/1422-0067/23/11/6288mesenchymal stem cellsosteogenic differentiationbasal cell culture mediaDMEM<sup>99m</sup>Tc-HDP labelinghydroxyapatite |
| spellingShingle | Tobias Grossner Uwe Haberkorn Jakob Hofmann Tobias Gotterbarm Effects of Different Basal Cell Culture Media upon the Osteogenic Response of hMSCs Evaluated by <sup>99m</sup>Tc-HDP Labeling International Journal of Molecular Sciences mesenchymal stem cells osteogenic differentiation basal cell culture media DMEM <sup>99m</sup>Tc-HDP labeling hydroxyapatite |
| title | Effects of Different Basal Cell Culture Media upon the Osteogenic Response of hMSCs Evaluated by <sup>99m</sup>Tc-HDP Labeling |
| title_full | Effects of Different Basal Cell Culture Media upon the Osteogenic Response of hMSCs Evaluated by <sup>99m</sup>Tc-HDP Labeling |
| title_fullStr | Effects of Different Basal Cell Culture Media upon the Osteogenic Response of hMSCs Evaluated by <sup>99m</sup>Tc-HDP Labeling |
| title_full_unstemmed | Effects of Different Basal Cell Culture Media upon the Osteogenic Response of hMSCs Evaluated by <sup>99m</sup>Tc-HDP Labeling |
| title_short | Effects of Different Basal Cell Culture Media upon the Osteogenic Response of hMSCs Evaluated by <sup>99m</sup>Tc-HDP Labeling |
| title_sort | effects of different basal cell culture media upon the osteogenic response of hmscs evaluated by sup 99m sup tc hdp labeling |
| topic | mesenchymal stem cells osteogenic differentiation basal cell culture media DMEM <sup>99m</sup>Tc-HDP labeling hydroxyapatite |
| url | https://www.mdpi.com/1422-0067/23/11/6288 |
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