Identification, characterization and functional analysis of AGAMOUS subfamily genes associated with floral organs and seed development in Marigold (Tagetes erecta)
Abstract Background AGAMOUS (AG) subfamily genes regulate the floral organs initiation and development, fruit and seed development. At present, there has been insufficient study of the function of AG subfamily genes in Asteraceae. Marigold (Tagetes erecta) belongs to Asteraceae family whose unique i...
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-020-02644-5 |
| _version_ | 1819172078459289600 |
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| author | Chunling Zhang Ludan Wei Wenjing Wang Wenquan Qi Zhe Cao Hang Li Manzhu Bao Yanhong He |
| author_facet | Chunling Zhang Ludan Wei Wenjing Wang Wenquan Qi Zhe Cao Hang Li Manzhu Bao Yanhong He |
| author_sort | Chunling Zhang |
| collection | DOAJ |
| description | Abstract Background AGAMOUS (AG) subfamily genes regulate the floral organs initiation and development, fruit and seed development. At present, there has been insufficient study of the function of AG subfamily genes in Asteraceae. Marigold (Tagetes erecta) belongs to Asteraceae family whose unique inflorescence structure makes it an important research target for understanding floral organ development in plants. Results Four AG subfamily genes of marigold were isolated and phylogenetically grouped into class C (TeAG1 and TeAG2) and class D (TeAGL11–1 and TeAGL11–2) genes. Expression profile analysis demonstrated that these four genes were highly expressed in reproductive organs of marigold. Subcellular localization analysis suggested that all these four proteins were located in the nucleus. Protein-protein interactions analysis indicated that class C proteins had a wider interaction manner than class D proteins. Function analysis of ectopic expression in Arabidopsis thaliana revealed that TeAG1 displayed a C function specifying the stamen identity and carpel identity, and that TeAGL11–1 exhibited a D function regulating seed development and petal development. In addition, overexpression of both TeAG1 and TeAGL11–1 leaded to curling rosette leaf and early flowering in Arabidopsis thaliana. Conclusions This study provides an insight into molecular mechanism of AG subfamily genes in Asteraceae species and technical support for improvement of several floral traits. |
| first_indexed | 2024-12-22T20:01:28Z |
| format | Article |
| id | doaj.art-6c76931606f24322bbe7ec6fc5bb9c9e |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-22T20:01:28Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-6c76931606f24322bbe7ec6fc5bb9c9e2022-12-21T18:14:17ZengBMCBMC Plant Biology1471-22292020-09-0120111710.1186/s12870-020-02644-5Identification, characterization and functional analysis of AGAMOUS subfamily genes associated with floral organs and seed development in Marigold (Tagetes erecta)Chunling Zhang0Ludan Wei1Wenjing Wang2Wenquan Qi3Zhe Cao4Hang Li5Manzhu Bao6Yanhong He7Key Laboratory of Horticultural Plant Biology, Ministry of Education; College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityKey Laboratory of Horticultural Plant Biology, Ministry of Education; College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityKey Laboratory of Horticultural Plant Biology, Ministry of Education; College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityKey Laboratory of Horticultural Plant Biology, Ministry of Education; College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityCrop Development Centre/Department of Plant Sciences, University of SaskatchewanKey Laboratory of Horticultural Plant Biology, Ministry of Education; College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityKey Laboratory of Horticultural Plant Biology, Ministry of Education; College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityKey Laboratory of Horticultural Plant Biology, Ministry of Education; College of Horticulture and Forestry Sciences, Huazhong Agricultural UniversityAbstract Background AGAMOUS (AG) subfamily genes regulate the floral organs initiation and development, fruit and seed development. At present, there has been insufficient study of the function of AG subfamily genes in Asteraceae. Marigold (Tagetes erecta) belongs to Asteraceae family whose unique inflorescence structure makes it an important research target for understanding floral organ development in plants. Results Four AG subfamily genes of marigold were isolated and phylogenetically grouped into class C (TeAG1 and TeAG2) and class D (TeAGL11–1 and TeAGL11–2) genes. Expression profile analysis demonstrated that these four genes were highly expressed in reproductive organs of marigold. Subcellular localization analysis suggested that all these four proteins were located in the nucleus. Protein-protein interactions analysis indicated that class C proteins had a wider interaction manner than class D proteins. Function analysis of ectopic expression in Arabidopsis thaliana revealed that TeAG1 displayed a C function specifying the stamen identity and carpel identity, and that TeAGL11–1 exhibited a D function regulating seed development and petal development. In addition, overexpression of both TeAG1 and TeAGL11–1 leaded to curling rosette leaf and early flowering in Arabidopsis thaliana. Conclusions This study provides an insight into molecular mechanism of AG subfamily genes in Asteraceae species and technical support for improvement of several floral traits.http://link.springer.com/article/10.1186/s12870-020-02644-5MarigoldFloral organsMADS-box genesAGAMOUS subfamily genesFunctional analysis |
| spellingShingle | Chunling Zhang Ludan Wei Wenjing Wang Wenquan Qi Zhe Cao Hang Li Manzhu Bao Yanhong He Identification, characterization and functional analysis of AGAMOUS subfamily genes associated with floral organs and seed development in Marigold (Tagetes erecta) BMC Plant Biology Marigold Floral organs MADS-box genes AGAMOUS subfamily genes Functional analysis |
| title | Identification, characterization and functional analysis of AGAMOUS subfamily genes associated with floral organs and seed development in Marigold (Tagetes erecta) |
| title_full | Identification, characterization and functional analysis of AGAMOUS subfamily genes associated with floral organs and seed development in Marigold (Tagetes erecta) |
| title_fullStr | Identification, characterization and functional analysis of AGAMOUS subfamily genes associated with floral organs and seed development in Marigold (Tagetes erecta) |
| title_full_unstemmed | Identification, characterization and functional analysis of AGAMOUS subfamily genes associated with floral organs and seed development in Marigold (Tagetes erecta) |
| title_short | Identification, characterization and functional analysis of AGAMOUS subfamily genes associated with floral organs and seed development in Marigold (Tagetes erecta) |
| title_sort | identification characterization and functional analysis of agamous subfamily genes associated with floral organs and seed development in marigold tagetes erecta |
| topic | Marigold Floral organs MADS-box genes AGAMOUS subfamily genes Functional analysis |
| url | http://link.springer.com/article/10.1186/s12870-020-02644-5 |
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