Effect of gamma irradiation on Pseudomonas syringae pv. tomato DC3000 - short communication
Gamma irradiation (GI) was evaluated for its in vitro and in vivo antibacterial activity against bacterial specks of tomato, Pseudomonas syringae pv. tomato (Pst). GI showed complete inactivation of Pst DC3000, especially at a dose of 200 Gy in vitro. Gamma-irradiated bacterial cells were found to (...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2016-06-01
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| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-201602-0004_effect-of-gamma-irradiation-on-pseudomonas-syringae-pv-tomato-dc3000-short-communication.php |
| _version_ | 1797898488687624192 |
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| author | Rae-Dong Jeong Eun-Hee Chu Gun Woong Lee Jeong Mee Park Hae-Jun Park |
| author_facet | Rae-Dong Jeong Eun-Hee Chu Gun Woong Lee Jeong Mee Park Hae-Jun Park |
| author_sort | Rae-Dong Jeong |
| collection | DOAJ |
| description | Gamma irradiation (GI) was evaluated for its in vitro and in vivo antibacterial activity against bacterial specks of tomato, Pseudomonas syringae pv. tomato (Pst). GI showed complete inactivation of Pst DC3000, especially at a dose of 200 Gy in vitro. Gamma-irradiated bacterial cells were found to (1) have spilled cytoplasmic contents, (2) display a damage on the surface of the cells, (3) have reduced membrane integrity, and (4) have fragmented genomic DNA, all in a dose-dependent manner. Consistent with the in vitro assay, a low dose of 150 Gy showed sufficient antibacterial activity on tomato seedlings. The present study suggested that the GI of bacterial cells results in substantial damage of the cell membrane, and that, along with DNA fragmentation, results in dose-dependent cell inactivation. These findings suggest that GI has potential as an antibacterial approach to reduce the severity of the bacterial speck disease of tomato. |
| first_indexed | 2024-04-10T08:15:11Z |
| format | Article |
| id | doaj.art-6c7bec7b4db3499b895fa439ab850c51 |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T08:15:11Z |
| publishDate | 2016-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-6c7bec7b4db3499b895fa439ab850c512023-02-23T03:44:17ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412016-06-0152210711210.17221/68/2015-PPSpps-201602-0004Effect of gamma irradiation on Pseudomonas syringae pv. tomato DC3000 - short communicationRae-Dong Jeong0Eun-Hee Chu1Gun Woong Lee2Jeong Mee Park3Hae-Jun Park4Research Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of KoreaResearch Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of KoreaResearch Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of KoreaPlant Systems Engineering Research Center, KRIBB, Daejeon, Republic of KoreaResearch Division for Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, Republic of KoreaGamma irradiation (GI) was evaluated for its in vitro and in vivo antibacterial activity against bacterial specks of tomato, Pseudomonas syringae pv. tomato (Pst). GI showed complete inactivation of Pst DC3000, especially at a dose of 200 Gy in vitro. Gamma-irradiated bacterial cells were found to (1) have spilled cytoplasmic contents, (2) display a damage on the surface of the cells, (3) have reduced membrane integrity, and (4) have fragmented genomic DNA, all in a dose-dependent manner. Consistent with the in vitro assay, a low dose of 150 Gy showed sufficient antibacterial activity on tomato seedlings. The present study suggested that the GI of bacterial cells results in substantial damage of the cell membrane, and that, along with DNA fragmentation, results in dose-dependent cell inactivation. These findings suggest that GI has potential as an antibacterial approach to reduce the severity of the bacterial speck disease of tomato.https://pps.agriculturejournals.cz/artkey/pps-201602-0004_effect-of-gamma-irradiation-on-pseudomonas-syringae-pv-tomato-dc3000-short-communication.phpantibacterial activitybacterial speck of tomatoionizing radiation |
| spellingShingle | Rae-Dong Jeong Eun-Hee Chu Gun Woong Lee Jeong Mee Park Hae-Jun Park Effect of gamma irradiation on Pseudomonas syringae pv. tomato DC3000 - short communication Plant Protection Science antibacterial activity bacterial speck of tomato ionizing radiation |
| title | Effect of gamma irradiation on Pseudomonas syringae pv. tomato DC3000 - short communication |
| title_full | Effect of gamma irradiation on Pseudomonas syringae pv. tomato DC3000 - short communication |
| title_fullStr | Effect of gamma irradiation on Pseudomonas syringae pv. tomato DC3000 - short communication |
| title_full_unstemmed | Effect of gamma irradiation on Pseudomonas syringae pv. tomato DC3000 - short communication |
| title_short | Effect of gamma irradiation on Pseudomonas syringae pv. tomato DC3000 - short communication |
| title_sort | effect of gamma irradiation on pseudomonas syringae pv tomato dc3000 short communication |
| topic | antibacterial activity bacterial speck of tomato ionizing radiation |
| url | https://pps.agriculturejournals.cz/artkey/pps-201602-0004_effect-of-gamma-irradiation-on-pseudomonas-syringae-pv-tomato-dc3000-short-communication.php |
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