Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico Approach
The main food-origin antigens that the infant’s body is in contact with are cow’s milk proteins (CMP). Still, CMP are one of the main sources of beneficial biologically active peptides that play a role in treatment of non-communicable diseases. Safe methods to quickly predict the sensitizing potenti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/1/163 |
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| author | Anna Maria Ogrodowczyk Ivan Dimitrov Barbara Wróblewska |
| author_facet | Anna Maria Ogrodowczyk Ivan Dimitrov Barbara Wróblewska |
| author_sort | Anna Maria Ogrodowczyk |
| collection | DOAJ |
| description | The main food-origin antigens that the infant’s body is in contact with are cow’s milk proteins (CMP). Still, CMP are one of the main sources of beneficial biologically active peptides that play a role in treatment of non-communicable diseases. Safe methods to quickly predict the sensitizing potential of food proteins among their range of health-promoting properties are essential. The aim of study was to adapt an integrated approach combining several <i>in silico</i> (<i>IS</i>) studies and <i>in vitro</i> (<i>IV</i>) assays to screen the multifunctionality of CMP-derived peptides. Major histocompatability complex type II MHC II-binders, interleukin-4 and -10 inducers, interferon γ -inducers and immunobioactivity tools were used to predict the peptide-power of inducing allergies or tolerance. A comparison of the peptide profiless revealed the presence of one identical and one overlapping sequence in <i>IS</i> and <i>IV</i> hydrolysate. By <i>IS</i> analysis, four of 24 peptides were found to have high affinity and stimulate IL-4 expression, and by <i>IV,</i> one of seven peptides had this potential (Bos d9 peptide DIPNPIGSENSEK (195–208)). Three <i>IV</i> peptides may induce IL-10 expression. The <i>IV</i>/<i>IS</i> assessment seems promising agents for peptides’ potential determination dedicated only to preliminary screening of peptides. The <i>IV</i> verification is still crucial in further steps of studies. |
| first_indexed | 2024-03-09T04:48:53Z |
| format | Article |
| id | doaj.art-6c7c516974004934bc8d617b25d2b0b0 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T04:48:53Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-6c7c516974004934bc8d617b25d2b0b02023-12-03T13:12:00ZengMDPI AGFoods2304-81582021-01-0110116310.3390/foods10010163Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico ApproachAnna Maria Ogrodowczyk0Ivan Dimitrov1Barbara Wróblewska2Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Department of Immunology and Food Microbiology, Tuwima 10, 10-748 Olsztyn, PolandFaculty of Pharmacy, Medical University of Sofia, 1000 Sofia, BulgariaInstitute of Animal Reproduction and Food Research of Polish Academy of Sciences, Department of Immunology and Food Microbiology, Tuwima 10, 10-748 Olsztyn, PolandThe main food-origin antigens that the infant’s body is in contact with are cow’s milk proteins (CMP). Still, CMP are one of the main sources of beneficial biologically active peptides that play a role in treatment of non-communicable diseases. Safe methods to quickly predict the sensitizing potential of food proteins among their range of health-promoting properties are essential. The aim of study was to adapt an integrated approach combining several <i>in silico</i> (<i>IS</i>) studies and <i>in vitro</i> (<i>IV</i>) assays to screen the multifunctionality of CMP-derived peptides. Major histocompatability complex type II MHC II-binders, interleukin-4 and -10 inducers, interferon γ -inducers and immunobioactivity tools were used to predict the peptide-power of inducing allergies or tolerance. A comparison of the peptide profiless revealed the presence of one identical and one overlapping sequence in <i>IS</i> and <i>IV</i> hydrolysate. By <i>IS</i> analysis, four of 24 peptides were found to have high affinity and stimulate IL-4 expression, and by <i>IV,</i> one of seven peptides had this potential (Bos d9 peptide DIPNPIGSENSEK (195–208)). Three <i>IV</i> peptides may induce IL-10 expression. The <i>IV</i>/<i>IS</i> assessment seems promising agents for peptides’ potential determination dedicated only to preliminary screening of peptides. The <i>IV</i> verification is still crucial in further steps of studies.https://www.mdpi.com/2304-8158/10/1/163allergycow’s milk allergens<i>in silico</i> toolsMHC II binded peptidesIL-4 inducing peptidesIFNγ inducing peptides |
| spellingShingle | Anna Maria Ogrodowczyk Ivan Dimitrov Barbara Wróblewska Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico Approach Foods allergy cow’s milk allergens <i>in silico</i> tools MHC II binded peptides IL-4 inducing peptides IFNγ inducing peptides |
| title | Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico Approach |
| title_full | Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico Approach |
| title_fullStr | Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico Approach |
| title_full_unstemmed | Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico Approach |
| title_short | Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact—Integrated In Vitro/In Silico Approach |
| title_sort | two faces of milk proteins peptides with both allergenic and multidimensional health beneficial impact integrated in vitro in silico approach |
| topic | allergy cow’s milk allergens <i>in silico</i> tools MHC II binded peptides IL-4 inducing peptides IFNγ inducing peptides |
| url | https://www.mdpi.com/2304-8158/10/1/163 |
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