Environmental <i>Streptococcus uberis</i> Associated with Clinical Mastitis in Dairy Cows: Virulence Traits, Antimicrobial and Biocide Resistance, and Epidemiological Typing
Mastitis remains a serious problem for dairy animals. The misappropriation of antimicrobial agents helps accelerate resistance, which poses a serious challenge in controlling environmental <i>S. uberis</i> infection. Here, we study the virulence attributes, antimicrobial and biocide resi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/7/1849 |
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| author | Norhan K. Abd El-Aziz Ahmed M. Ammar Hend M. El Damaty Rehab A. Abd Elkader Hosam A. Saad Waleed El-Kazzaz Eman Khalifa |
| author_facet | Norhan K. Abd El-Aziz Ahmed M. Ammar Hend M. El Damaty Rehab A. Abd Elkader Hosam A. Saad Waleed El-Kazzaz Eman Khalifa |
| author_sort | Norhan K. Abd El-Aziz |
| collection | DOAJ |
| description | Mastitis remains a serious problem for dairy animals. The misappropriation of antimicrobial agents helps accelerate resistance, which poses a serious challenge in controlling environmental <i>S. uberis</i> infection. Here, we study the virulence attributes, antimicrobial and biocide resistance, and epidemiological typing of <i>S. uberis</i> recovered from bovine clinical mastitis in dairy farms of diverse hygienic interventions in Egypt. The overall <i>S. uberis</i> infection rate was 20.59%; all were multidrug-resistant (MDR). The <i>sua</i> gene was the most frequent virulence gene (42.02%), followed by <i>pau</i>A (40.57%), <i>cfu</i> (21.73%), <i>skc</i> (20.28%), and <i>opp</i> (11.59%). The <i>erm</i>(B) gene served as the predominant antimicrobial-resistant gene (75.36%), followed by <i>fexA</i> (52.63%) and <i>tet</i>(M), <i>blaZ</i>, and <i>aac(6′)aph(2″)</i> genes (46.38% each). Of note, 79.71%, 78.26%, and 18.84% of <i>S. uberis</i> isolates harbored <i>qacED1</i>, <i>qacC/D</i>, and <i>qacA/B</i> genes, respectively. All analyzed isolates were <i>S. uberis</i> type I by their unique RFLP–PCR pattern. In conclusion, the sustained presence of <i>pauA</i> and <i>sua</i> genes throughout the investigated farms contributes to a better understanding of the bacterium’s pathogenicity. Furthermore, MDR coupled with the existence of biocide resistance genes indicates the importance of <i>S. uberis</i> surveillance and the prudent use of antimicrobials in veterinary clinical medicine to avoid the dissemination of antimicrobial resistance. |
| first_indexed | 2024-03-10T10:11:41Z |
| format | Article |
| id | doaj.art-6c7ca99ee1bb470c829b50980fe2a630 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T10:11:41Z |
| publishDate | 2021-06-01 |
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| record_format | Article |
| series | Animals |
| spelling | doaj.art-6c7ca99ee1bb470c829b50980fe2a6302023-11-22T01:08:47ZengMDPI AGAnimals2076-26152021-06-01117184910.3390/ani11071849Environmental <i>Streptococcus uberis</i> Associated with Clinical Mastitis in Dairy Cows: Virulence Traits, Antimicrobial and Biocide Resistance, and Epidemiological TypingNorhan K. Abd El-Aziz0Ahmed M. Ammar1Hend M. El Damaty2Rehab A. Abd Elkader3Hosam A. Saad4Waleed El-Kazzaz5Eman Khalifa6Department of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Microbiology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptDepartment of Animal Medicine, Infectious Diseases, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, EgyptBelbies Veterinary Organization, Ministry of Agriculture, Belbies 44974, EgyptDepartment of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi ArabiaMolecular Microbiology Lab., Botany and Microbiology Department, Faculty of Science, Suez Canal University, Ismailia 41522, EgyptDepartment of Microbiology, Faculty of Veterinary Medicine, Matrouh University, Matrouh 51511, EgyptMastitis remains a serious problem for dairy animals. The misappropriation of antimicrobial agents helps accelerate resistance, which poses a serious challenge in controlling environmental <i>S. uberis</i> infection. Here, we study the virulence attributes, antimicrobial and biocide resistance, and epidemiological typing of <i>S. uberis</i> recovered from bovine clinical mastitis in dairy farms of diverse hygienic interventions in Egypt. The overall <i>S. uberis</i> infection rate was 20.59%; all were multidrug-resistant (MDR). The <i>sua</i> gene was the most frequent virulence gene (42.02%), followed by <i>pau</i>A (40.57%), <i>cfu</i> (21.73%), <i>skc</i> (20.28%), and <i>opp</i> (11.59%). The <i>erm</i>(B) gene served as the predominant antimicrobial-resistant gene (75.36%), followed by <i>fexA</i> (52.63%) and <i>tet</i>(M), <i>blaZ</i>, and <i>aac(6′)aph(2″)</i> genes (46.38% each). Of note, 79.71%, 78.26%, and 18.84% of <i>S. uberis</i> isolates harbored <i>qacED1</i>, <i>qacC/D</i>, and <i>qacA/B</i> genes, respectively. All analyzed isolates were <i>S. uberis</i> type I by their unique RFLP–PCR pattern. In conclusion, the sustained presence of <i>pauA</i> and <i>sua</i> genes throughout the investigated farms contributes to a better understanding of the bacterium’s pathogenicity. Furthermore, MDR coupled with the existence of biocide resistance genes indicates the importance of <i>S. uberis</i> surveillance and the prudent use of antimicrobials in veterinary clinical medicine to avoid the dissemination of antimicrobial resistance.https://www.mdpi.com/2076-2615/11/7/1849antimicrobial resistancebiocidesdairy cowsintramammary infectionsRFLP–PCR<i>Streptococcus uberis</i> |
| spellingShingle | Norhan K. Abd El-Aziz Ahmed M. Ammar Hend M. El Damaty Rehab A. Abd Elkader Hosam A. Saad Waleed El-Kazzaz Eman Khalifa Environmental <i>Streptococcus uberis</i> Associated with Clinical Mastitis in Dairy Cows: Virulence Traits, Antimicrobial and Biocide Resistance, and Epidemiological Typing Animals antimicrobial resistance biocides dairy cows intramammary infections RFLP–PCR <i>Streptococcus uberis</i> |
| title | Environmental <i>Streptococcus uberis</i> Associated with Clinical Mastitis in Dairy Cows: Virulence Traits, Antimicrobial and Biocide Resistance, and Epidemiological Typing |
| title_full | Environmental <i>Streptococcus uberis</i> Associated with Clinical Mastitis in Dairy Cows: Virulence Traits, Antimicrobial and Biocide Resistance, and Epidemiological Typing |
| title_fullStr | Environmental <i>Streptococcus uberis</i> Associated with Clinical Mastitis in Dairy Cows: Virulence Traits, Antimicrobial and Biocide Resistance, and Epidemiological Typing |
| title_full_unstemmed | Environmental <i>Streptococcus uberis</i> Associated with Clinical Mastitis in Dairy Cows: Virulence Traits, Antimicrobial and Biocide Resistance, and Epidemiological Typing |
| title_short | Environmental <i>Streptococcus uberis</i> Associated with Clinical Mastitis in Dairy Cows: Virulence Traits, Antimicrobial and Biocide Resistance, and Epidemiological Typing |
| title_sort | environmental i streptococcus uberis i associated with clinical mastitis in dairy cows virulence traits antimicrobial and biocide resistance and epidemiological typing |
| topic | antimicrobial resistance biocides dairy cows intramammary infections RFLP–PCR <i>Streptococcus uberis</i> |
| url | https://www.mdpi.com/2076-2615/11/7/1849 |
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