Molecular detection of plasmid-mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae during Covid-19 pandemic
Plasmid-mediated quinolone resistance (PMQR) genes confer low resistance to Fluoroquinolones (FQs). This study aims to detect five PMQR genes among FQs-resistant Klebsiella pneumoniae isolated from various clinical specimens. Out of 120 K. pneumoniae isolates, 68 FQs-resistance K. pneumoniae were in...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2023-03-01
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| Series: | Pharmacia |
| Online Access: | https://pharmacia.pensoft.net/article/90610/download/pdf/ |
| _version_ | 1797861968574414848 |
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| author | Mustafa M. Abdulkareem Masood A. Abdulrahman Najim A. Yassin |
| author_facet | Mustafa M. Abdulkareem Masood A. Abdulrahman Najim A. Yassin |
| author_sort | Mustafa M. Abdulkareem |
| collection | DOAJ |
| description | Plasmid-mediated quinolone resistance (PMQR) genes confer low resistance to Fluoroquinolones (FQs). This study aims to detect five PMQR genes among FQs-resistant Klebsiella pneumoniae isolated from various clinical specimens. Out of 120 K. pneumoniae isolates, 68 FQs-resistance K. pneumoniae were included in a molecular study. Standard microbiological tests were used for identification and antimicrobial susceptibility. For the detection of PMQR genes, conventional polymerase chain reaction was used. A molecular study revealed that (73.5%) of samples harbored PMQR genes, and among them, 58% were co-carriages of PMQR gene variants. Aac (6’)-Ib-cr gene was predominant (47.1%) among samples, and qepA had the lowest percentage (11.8%), qnr genes were (32.4%) (29.4%) (20.6%) qnrS, qnrB, and qnrA respectively. Overall, high percentages of PMQR genes were detected, and almost all of samples were phenotypically resistant to ciprofloxacin. As well, there was a significant statistical relationship between phenotypically ESBL-producers and qnrB and qepA genes. |
| first_indexed | 2024-04-09T22:11:47Z |
| format | Article |
| id | doaj.art-6c7e2dbe90bd4f24a801bf70764008c9 |
| institution | Directory Open Access Journal |
| issn | 2603-557X |
| language | English |
| last_indexed | 2024-04-09T22:11:47Z |
| publishDate | 2023-03-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | Pharmacia |
| spelling | doaj.art-6c7e2dbe90bd4f24a801bf70764008c92023-03-23T09:11:11ZengPensoft PublishersPharmacia2603-557X2023-03-0170122523110.3897/pharmacia.70.e9061090610Molecular detection of plasmid-mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae during Covid-19 pandemicMustafa M. Abdulkareem0Masood A. Abdulrahman1Najim A. Yassin2Duhok Polytechnic UniversityDuhok Polytechnic UniversityDuhok UniversityPlasmid-mediated quinolone resistance (PMQR) genes confer low resistance to Fluoroquinolones (FQs). This study aims to detect five PMQR genes among FQs-resistant Klebsiella pneumoniae isolated from various clinical specimens. Out of 120 K. pneumoniae isolates, 68 FQs-resistance K. pneumoniae were included in a molecular study. Standard microbiological tests were used for identification and antimicrobial susceptibility. For the detection of PMQR genes, conventional polymerase chain reaction was used. A molecular study revealed that (73.5%) of samples harbored PMQR genes, and among them, 58% were co-carriages of PMQR gene variants. Aac (6’)-Ib-cr gene was predominant (47.1%) among samples, and qepA had the lowest percentage (11.8%), qnr genes were (32.4%) (29.4%) (20.6%) qnrS, qnrB, and qnrA respectively. Overall, high percentages of PMQR genes were detected, and almost all of samples were phenotypically resistant to ciprofloxacin. As well, there was a significant statistical relationship between phenotypically ESBL-producers and qnrB and qepA genes.https://pharmacia.pensoft.net/article/90610/download/pdf/ |
| spellingShingle | Mustafa M. Abdulkareem Masood A. Abdulrahman Najim A. Yassin Molecular detection of plasmid-mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae during Covid-19 pandemic Pharmacia |
| title | Molecular detection of plasmid-mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae during Covid-19 pandemic |
| title_full | Molecular detection of plasmid-mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae during Covid-19 pandemic |
| title_fullStr | Molecular detection of plasmid-mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae during Covid-19 pandemic |
| title_full_unstemmed | Molecular detection of plasmid-mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae during Covid-19 pandemic |
| title_short | Molecular detection of plasmid-mediated quinolone resistance genes among clinical isolates of Klebsiella pneumoniae during Covid-19 pandemic |
| title_sort | molecular detection of plasmid mediated quinolone resistance genes among clinical isolates of klebsiella pneumoniae during covid 19 pandemic |
| url | https://pharmacia.pensoft.net/article/90610/download/pdf/ |
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