Experimental model of heterotopic ossification in Wistar rats
Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues adjacent to large joints, resulting in joint mobility deficit. In order to determine which treatment techniques are more appropriate for such condition, experimental models of induce...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2012-06-01
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| Series: | Brazilian Journal of Medical and Biological Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600004 |
| _version_ | 1818273274613727232 |
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| author | T.G.G. Zotz J.B. de Paula A.D.L. Moser |
| author_facet | T.G.G. Zotz J.B. de Paula A.D.L. Moser |
| author_sort | T.G.G. Zotz |
| collection | DOAJ |
| description | Heterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues adjacent to large joints, resulting in joint mobility deficit. In order to determine which treatment techniques are more appropriate for such condition, experimental models of induced heterotopic bone formation have been proposed using heterologous demineralized bone matrix implants and bone morphogenetic protein and other tissues. The objective of the present experimental study was to identify a reliable protocol to induce HO in Wistar rats, based on autologous bone marrow (BM) implantation, comparing 3 different BM volumes and based on literature evidence of this HO induction model in larger laboratory animals. Twelve male Wistar albino rats weighing 350/390 g were used. The animals were anesthetized for blood sampling before HO induction in order to quantify serum alkaline phosphatase (ALP). HO was induced by BM implantation in both quadriceps muscles of these animals, experimental group (EG). Thirty-five days after the induction, another blood sample was collected for ALP determination. The results showed a weight gain in the EG and no significant difference in ALP levels when comparing the periods before and after induction. Qualitative histological analysis confirmed the occurrence of heterotopic ossification in all 12 EG rats. In conclusion, the HO induction model was effective when 0.35 mL autologous BM was applied to the quadriceps of Wistar rats. |
| first_indexed | 2024-12-12T21:55:22Z |
| format | Article |
| id | doaj.art-6c7f76e373c64dec866ac41d5f74b33c |
| institution | Directory Open Access Journal |
| issn | 0100-879X 1414-431X |
| language | English |
| last_indexed | 2024-12-12T21:55:22Z |
| publishDate | 2012-06-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-6c7f76e373c64dec866ac41d5f74b33c2022-12-22T00:10:39ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research0100-879X1414-431X2012-06-01456497501Experimental model of heterotopic ossification in Wistar ratsT.G.G. ZotzJ.B. de PaulaA.D.L. MoserHeterotopic ossification (HO) is a metaplastic biological process in which there is newly formed bone in soft tissues adjacent to large joints, resulting in joint mobility deficit. In order to determine which treatment techniques are more appropriate for such condition, experimental models of induced heterotopic bone formation have been proposed using heterologous demineralized bone matrix implants and bone morphogenetic protein and other tissues. The objective of the present experimental study was to identify a reliable protocol to induce HO in Wistar rats, based on autologous bone marrow (BM) implantation, comparing 3 different BM volumes and based on literature evidence of this HO induction model in larger laboratory animals. Twelve male Wistar albino rats weighing 350/390 g were used. The animals were anesthetized for blood sampling before HO induction in order to quantify serum alkaline phosphatase (ALP). HO was induced by BM implantation in both quadriceps muscles of these animals, experimental group (EG). Thirty-five days after the induction, another blood sample was collected for ALP determination. The results showed a weight gain in the EG and no significant difference in ALP levels when comparing the periods before and after induction. Qualitative histological analysis confirmed the occurrence of heterotopic ossification in all 12 EG rats. In conclusion, the HO induction model was effective when 0.35 mL autologous BM was applied to the quadriceps of Wistar rats.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600004Heterotopic ossificationAutologous bone marrowSerum alkaline phosphatase |
| spellingShingle | T.G.G. Zotz J.B. de Paula A.D.L. Moser Experimental model of heterotopic ossification in Wistar rats Brazilian Journal of Medical and Biological Research Heterotopic ossification Autologous bone marrow Serum alkaline phosphatase |
| title | Experimental model of heterotopic ossification in Wistar rats |
| title_full | Experimental model of heterotopic ossification in Wistar rats |
| title_fullStr | Experimental model of heterotopic ossification in Wistar rats |
| title_full_unstemmed | Experimental model of heterotopic ossification in Wistar rats |
| title_short | Experimental model of heterotopic ossification in Wistar rats |
| title_sort | experimental model of heterotopic ossification in wistar rats |
| topic | Heterotopic ossification Autologous bone marrow Serum alkaline phosphatase |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000600004 |
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