Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics.
The first genotyping data on measles virus (MeV) strains in Cameroon dates from 1994, while other studies were realized in 2001 and 2011 with the establishment of MeV virological surveillance. However, the genetic data of MeV strains circulating in Cameroon remains fragmented and concentrated in cer...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0222428 |
| _version_ | 1819128350540562432 |
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| author | Franck-Martin Obam Mekanda Chavely Gwladys Monamele Frédy Brice Simo Nemg Gilde Martial Yonga Diane Ouapi Véronique Penlap Beng Christophe Batéjat Valérie Caro Jean-Claude Manuguerra Maurice Demanou |
| author_facet | Franck-Martin Obam Mekanda Chavely Gwladys Monamele Frédy Brice Simo Nemg Gilde Martial Yonga Diane Ouapi Véronique Penlap Beng Christophe Batéjat Valérie Caro Jean-Claude Manuguerra Maurice Demanou |
| author_sort | Franck-Martin Obam Mekanda |
| collection | DOAJ |
| description | The first genotyping data on measles virus (MeV) strains in Cameroon dates from 1994, while other studies were realized in 2001 and 2011 with the establishment of MeV virological surveillance. However, the genetic data of MeV strains circulating in Cameroon remains fragmented and concentrated in certain regions, hence the need for an update. The objective of this study was to have recent data on MeV genotypes circulating in Cameroon. Ninety throat swabs collected during recent measles outbreaks were analyzed by MeV genotyping RT-PCR using the nucleoprotein gene N. The resulting sequences were analyzed on the basis of 450 nucleotides with MEGA 7 software. Overall genome analysis was performed on 40/90 sequences. The strains were from all ten regions and all belonged to cluster 1 of genotype B3. The genotype B3 has been circulating in Cameroon for long periods of time; efforts must be made in immunization for its elimination. |
| first_indexed | 2024-12-22T08:26:26Z |
| format | Article |
| id | doaj.art-6c82729189e443fa89f2436cf366c6c8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T08:26:26Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6c82729189e443fa89f2436cf366c6c82022-12-21T18:32:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01149e022242810.1371/journal.pone.0222428Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics.Franck-Martin Obam MekandaChavely Gwladys MonameleFrédy Brice Simo NemgGilde Martial YongaDiane OuapiVéronique Penlap BengChristophe BatéjatValérie CaroJean-Claude ManuguerraMaurice DemanouThe first genotyping data on measles virus (MeV) strains in Cameroon dates from 1994, while other studies were realized in 2001 and 2011 with the establishment of MeV virological surveillance. However, the genetic data of MeV strains circulating in Cameroon remains fragmented and concentrated in certain regions, hence the need for an update. The objective of this study was to have recent data on MeV genotypes circulating in Cameroon. Ninety throat swabs collected during recent measles outbreaks were analyzed by MeV genotyping RT-PCR using the nucleoprotein gene N. The resulting sequences were analyzed on the basis of 450 nucleotides with MEGA 7 software. Overall genome analysis was performed on 40/90 sequences. The strains were from all ten regions and all belonged to cluster 1 of genotype B3. The genotype B3 has been circulating in Cameroon for long periods of time; efforts must be made in immunization for its elimination.https://doi.org/10.1371/journal.pone.0222428 |
| spellingShingle | Franck-Martin Obam Mekanda Chavely Gwladys Monamele Frédy Brice Simo Nemg Gilde Martial Yonga Diane Ouapi Véronique Penlap Beng Christophe Batéjat Valérie Caro Jean-Claude Manuguerra Maurice Demanou Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics. PLoS ONE |
| title | Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics. |
| title_full | Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics. |
| title_fullStr | Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics. |
| title_full_unstemmed | Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics. |
| title_short | Molecular characterization of measles virus strains circulating in Cameroon during the 2013-2016 epidemics. |
| title_sort | molecular characterization of measles virus strains circulating in cameroon during the 2013 2016 epidemics |
| url | https://doi.org/10.1371/journal.pone.0222428 |
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