Genomic Analysis of Detoxification Supergene Families in the Mosquito Anopheles sinensis.
Anopheles sinensis is an important malaria vector in China and other Southeast Asian countries, and the emergence of insecticide resistance in this mosquito poses a serious threat to the efficacy of malaria control programs. The recently published An. sinensis genome and transcriptome provide an opp...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4654499?pdf=render |
| _version_ | 1819039079815184384 |
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| author | Dan Zhou Xianmiao Liu Yan Sun Lei Ma Bo Shen Changliang Zhu |
| author_facet | Dan Zhou Xianmiao Liu Yan Sun Lei Ma Bo Shen Changliang Zhu |
| author_sort | Dan Zhou |
| collection | DOAJ |
| description | Anopheles sinensis is an important malaria vector in China and other Southeast Asian countries, and the emergence of insecticide resistance in this mosquito poses a serious threat to the efficacy of malaria control programs. The recently published An. sinensis genome and transcriptome provide an opportunity to understand the molecular mechanisms of insecticide resistance. Analysis of the An. sinensis genome revealed 174 detoxification genes, including 93 cytochrome P450s (P450s), 31 glutathione-S-transferases (GSTs), and 50 choline/carboxylesterases (CCEs). The gene number was similar to that in An. gambiae, but represented a decrease of 29% and 42% compared with Aedes aegypti and Culex quinquefasciatus, respectively. The considerable contraction in gene number in Anopheles mosquitoes mainly occurred in two detoxification supergene families, P450s and CCEs. The available An. sinensis transcriptome was also re-analyzed to further identify key resistance-associated detoxification genes. Among 174 detoxification genes, 124 (71%) were detected. Several candidate genes overexpressed in a deltamethrin-resistant strain (DR-strain) were identified as belonging to the CYP4 or CYP6 family of P450s and the Delta GST class. These generated data provide a basis for identifying the resistance-associated genes of An. sinensis at the molecular level. |
| first_indexed | 2024-12-21T08:47:30Z |
| format | Article |
| id | doaj.art-6c862f038aba478a8b6a906827489edc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T08:47:30Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-6c862f038aba478a8b6a906827489edc2022-12-21T19:09:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011011e014338710.1371/journal.pone.0143387Genomic Analysis of Detoxification Supergene Families in the Mosquito Anopheles sinensis.Dan ZhouXianmiao LiuYan SunLei MaBo ShenChangliang ZhuAnopheles sinensis is an important malaria vector in China and other Southeast Asian countries, and the emergence of insecticide resistance in this mosquito poses a serious threat to the efficacy of malaria control programs. The recently published An. sinensis genome and transcriptome provide an opportunity to understand the molecular mechanisms of insecticide resistance. Analysis of the An. sinensis genome revealed 174 detoxification genes, including 93 cytochrome P450s (P450s), 31 glutathione-S-transferases (GSTs), and 50 choline/carboxylesterases (CCEs). The gene number was similar to that in An. gambiae, but represented a decrease of 29% and 42% compared with Aedes aegypti and Culex quinquefasciatus, respectively. The considerable contraction in gene number in Anopheles mosquitoes mainly occurred in two detoxification supergene families, P450s and CCEs. The available An. sinensis transcriptome was also re-analyzed to further identify key resistance-associated detoxification genes. Among 174 detoxification genes, 124 (71%) were detected. Several candidate genes overexpressed in a deltamethrin-resistant strain (DR-strain) were identified as belonging to the CYP4 or CYP6 family of P450s and the Delta GST class. These generated data provide a basis for identifying the resistance-associated genes of An. sinensis at the molecular level.http://europepmc.org/articles/PMC4654499?pdf=render |
| spellingShingle | Dan Zhou Xianmiao Liu Yan Sun Lei Ma Bo Shen Changliang Zhu Genomic Analysis of Detoxification Supergene Families in the Mosquito Anopheles sinensis. PLoS ONE |
| title | Genomic Analysis of Detoxification Supergene Families in the Mosquito Anopheles sinensis. |
| title_full | Genomic Analysis of Detoxification Supergene Families in the Mosquito Anopheles sinensis. |
| title_fullStr | Genomic Analysis of Detoxification Supergene Families in the Mosquito Anopheles sinensis. |
| title_full_unstemmed | Genomic Analysis of Detoxification Supergene Families in the Mosquito Anopheles sinensis. |
| title_short | Genomic Analysis of Detoxification Supergene Families in the Mosquito Anopheles sinensis. |
| title_sort | genomic analysis of detoxification supergene families in the mosquito anopheles sinensis |
| url | http://europepmc.org/articles/PMC4654499?pdf=render |
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