Characterization of a new strain of Metarhizium novozealandicum with potential to be developed as a biopesticide
The fungal species Metarhizium novozealandicum, that occurs only in New Zealand and Australia has been poorly studied. In this work, a new strain of M. novozealandicum isolated from a larva of Wiseana sp. is described based on morphology, genomic multilocus (ITS, EF-1α and β-tubulin) phylogeny, gro...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-10-01
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| Series: | Mycology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21501203.2021.1935359 |
| _version_ | 1818426560428900352 |
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| author | Laura F. Villamizar Gloria Barrera Mark Hurst Travis R. Glare |
| author_facet | Laura F. Villamizar Gloria Barrera Mark Hurst Travis R. Glare |
| author_sort | Laura F. Villamizar |
| collection | DOAJ |
| description | The fungal species Metarhizium novozealandicum, that occurs only in New Zealand and Australia has been poorly studied. In this work, a new strain of M. novozealandicum isolated from a larva of Wiseana sp. is described based on morphology, genomic multilocus (ITS, EF-1α and β-tubulin) phylogeny, growth in different culture media and insecticidal activity. The isolate AgR-F177 was clustered in the same clade with M. novozealandicum. AgR-F177 colonies developed faster on Sabouraud Dextrose Agar (SDA) than on Potato Dextrose Agar (PDA) when incubated at 25°C, with no growth observed at 30°C on either media. Conidia yield on an oat-based medium in semisolid fermentation was 7.41 x 108conidia/g of substrate and a higher yield of 1.68 x 109conidia/g of substrate was obtained using solid fermentation on cooked rice. AgR-F177 formed microsclerotia (MS) in liquid fermentation after 7 days reaching the maximum yield of 3.3 × 103 MS/mL after 10 days. AgR-F177 caused mortality in Wiseana copularis, Costelytra giveni and Plutella xylostella larvae with efficacies up to 100%, 69.2%, and 45.7%, respectively. The ease of production of AgR-F177 with different fermentation systems and its pathogenicity against different insect pests reveal its potential as a new biopesticide. |
| first_indexed | 2024-12-14T14:31:46Z |
| format | Article |
| id | doaj.art-6c872a5a9a7e4422bac5f63a7ee98ff5 |
| institution | Directory Open Access Journal |
| issn | 2150-1203 2150-1211 |
| language | English |
| last_indexed | 2024-12-14T14:31:46Z |
| publishDate | 2021-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycology |
| spelling | doaj.art-6c872a5a9a7e4422bac5f63a7ee98ff52022-12-21T22:57:47ZengTaylor & Francis GroupMycology2150-12032150-12112021-10-0112426127810.1080/21501203.2021.19353591935359Characterization of a new strain of Metarhizium novozealandicum with potential to be developed as a biopesticideLaura F. Villamizar0Gloria Barrera1Mark Hurst2Travis R. Glare3Lincoln Research CentreCorporación Colombiana de Investigación AgropecuariaLincoln Research CentreBio-Protection Research Centre, Lincoln UniversityThe fungal species Metarhizium novozealandicum, that occurs only in New Zealand and Australia has been poorly studied. In this work, a new strain of M. novozealandicum isolated from a larva of Wiseana sp. is described based on morphology, genomic multilocus (ITS, EF-1α and β-tubulin) phylogeny, growth in different culture media and insecticidal activity. The isolate AgR-F177 was clustered in the same clade with M. novozealandicum. AgR-F177 colonies developed faster on Sabouraud Dextrose Agar (SDA) than on Potato Dextrose Agar (PDA) when incubated at 25°C, with no growth observed at 30°C on either media. Conidia yield on an oat-based medium in semisolid fermentation was 7.41 x 108conidia/g of substrate and a higher yield of 1.68 x 109conidia/g of substrate was obtained using solid fermentation on cooked rice. AgR-F177 formed microsclerotia (MS) in liquid fermentation after 7 days reaching the maximum yield of 3.3 × 103 MS/mL after 10 days. AgR-F177 caused mortality in Wiseana copularis, Costelytra giveni and Plutella xylostella larvae with efficacies up to 100%, 69.2%, and 45.7%, respectively. The ease of production of AgR-F177 with different fermentation systems and its pathogenicity against different insect pests reveal its potential as a new biopesticide.http://dx.doi.org/10.1080/21501203.2021.1935359metarhiziumentomopathogenic fungusporinagrass grubsdiamond back mothmicrosclerotia |
| spellingShingle | Laura F. Villamizar Gloria Barrera Mark Hurst Travis R. Glare Characterization of a new strain of Metarhizium novozealandicum with potential to be developed as a biopesticide Mycology metarhizium entomopathogenic fungus porina grass grubs diamond back moth microsclerotia |
| title | Characterization of a new strain of Metarhizium novozealandicum with potential to be developed as a biopesticide |
| title_full | Characterization of a new strain of Metarhizium novozealandicum with potential to be developed as a biopesticide |
| title_fullStr | Characterization of a new strain of Metarhizium novozealandicum with potential to be developed as a biopesticide |
| title_full_unstemmed | Characterization of a new strain of Metarhizium novozealandicum with potential to be developed as a biopesticide |
| title_short | Characterization of a new strain of Metarhizium novozealandicum with potential to be developed as a biopesticide |
| title_sort | characterization of a new strain of metarhizium novozealandicum with potential to be developed as a biopesticide |
| topic | metarhizium entomopathogenic fungus porina grass grubs diamond back moth microsclerotia |
| url | http://dx.doi.org/10.1080/21501203.2021.1935359 |
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