Plasma S1P and Sphingosine are not Different Prior to Pre-Eclampsia in Women at High Risk of Developing the Disease
Sphingolipids like sphingosine-1-phosphate (S1P) have been implicated in the pathophysiology of pre-eclampsia. We hypothesized that plasma S1P would be increased in women at high risk of developing pre-eclampsia who subsequently develop the disease. Low circulating placental growth factor (PlGF) is...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-01-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227522001456 |
| _version_ | 1797944075547049984 |
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| author | Edward D. Johnstone Melissa Westwood Mark Dilworth Jonathan R. Wray Alexandra C. Kendall Anna Nicolaou Jenny E. Myers |
| author_facet | Edward D. Johnstone Melissa Westwood Mark Dilworth Jonathan R. Wray Alexandra C. Kendall Anna Nicolaou Jenny E. Myers |
| author_sort | Edward D. Johnstone |
| collection | DOAJ |
| description | Sphingolipids like sphingosine-1-phosphate (S1P) have been implicated in the pathophysiology of pre-eclampsia. We hypothesized that plasma S1P would be increased in women at high risk of developing pre-eclampsia who subsequently develop the disease. Low circulating placental growth factor (PlGF) is known to be associated with development of pre-eclampsia; so further, we hypothesized that increased S1P would be associated with concurrently low PlGF. This was a case-control study using stored maternal blood samples from 14 to 24 weeks of pregnancy, collected from 95 women at increased risk of pre-eclampsia. Pregnancy outcome was classified as uncomplicated, preterm pre-eclampsia (<37 weeks), or term pre-eclampsia. Plasma lipids were extracted and analyzed by ultraperformance liquid chromatography coupled to electrospray ionization MS/MS to determine concentrations of S1P and sphingosine. Median plasma S1P was 0.339 nmol/ml, and median sphingosine was 6.77 nmol/l. There were no differences in the plasma concentrations of S1P or sphingosine in women who subsequently developed pre-eclampsia, no effect of gestational age, fetal sex, ethnicity, or the presence of pre-existing hypertension. There was a correlation between S1P and sphingosine plasma concentration (P < 0.0001). There was no relationship between S1P or sphingosine with PlGF. Previous studies have suggested that plasma S1P may be a biomarker of pre-eclampsia. In our larger study, we failed to demonstrate there are women at high risk of developing the disease. We did not show a relationship with known biomarkers of the disease, suggesting that S1P is unlikely to be a useful predictor of the development of pre-eclampsia later in pregnancy. |
| first_indexed | 2024-04-10T20:33:54Z |
| format | Article |
| id | doaj.art-6c879149de484f87af6c97aaf31f9ca7 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-04-10T20:33:54Z |
| publishDate | 2023-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-6c879149de484f87af6c97aaf31f9ca72023-01-25T04:14:40ZengElsevierJournal of Lipid Research0022-22752023-01-01641100312Plasma S1P and Sphingosine are not Different Prior to Pre-Eclampsia in Women at High Risk of Developing the DiseaseEdward D. Johnstone0Melissa Westwood1Mark Dilworth2Jonathan R. Wray3Alexandra C. Kendall4Anna Nicolaou5Jenny E. Myers6Maternal and Fetal Health Research Centre, School of Medical Sciences, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UK; For correspondence: Edward D. JohnstoneMaternal and Fetal Health Research Centre, School of Medical Sciences, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UKMaternal and Fetal Health Research Centre, School of Medical Sciences, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UKLaboratory for Lipidomics and Lipid Biology, Division of Pharmacy and Optometry, School of Health Sciences, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UKLaboratory for Lipidomics and Lipid Biology, Division of Pharmacy and Optometry, School of Health Sciences, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UKLaboratory for Lipidomics and Lipid Biology, Division of Pharmacy and Optometry, School of Health Sciences, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UK; Lydia Becker Institute of Immunology and Inflammation, Faculty of Biology, Medicine and Health, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UKMaternal and Fetal Health Research Centre, School of Medical Sciences, The University of Manchester, Manchester Academic Health Science Centre, Manchester, UKSphingolipids like sphingosine-1-phosphate (S1P) have been implicated in the pathophysiology of pre-eclampsia. We hypothesized that plasma S1P would be increased in women at high risk of developing pre-eclampsia who subsequently develop the disease. Low circulating placental growth factor (PlGF) is known to be associated with development of pre-eclampsia; so further, we hypothesized that increased S1P would be associated with concurrently low PlGF. This was a case-control study using stored maternal blood samples from 14 to 24 weeks of pregnancy, collected from 95 women at increased risk of pre-eclampsia. Pregnancy outcome was classified as uncomplicated, preterm pre-eclampsia (<37 weeks), or term pre-eclampsia. Plasma lipids were extracted and analyzed by ultraperformance liquid chromatography coupled to electrospray ionization MS/MS to determine concentrations of S1P and sphingosine. Median plasma S1P was 0.339 nmol/ml, and median sphingosine was 6.77 nmol/l. There were no differences in the plasma concentrations of S1P or sphingosine in women who subsequently developed pre-eclampsia, no effect of gestational age, fetal sex, ethnicity, or the presence of pre-existing hypertension. There was a correlation between S1P and sphingosine plasma concentration (P < 0.0001). There was no relationship between S1P or sphingosine with PlGF. Previous studies have suggested that plasma S1P may be a biomarker of pre-eclampsia. In our larger study, we failed to demonstrate there are women at high risk of developing the disease. We did not show a relationship with known biomarkers of the disease, suggesting that S1P is unlikely to be a useful predictor of the development of pre-eclampsia later in pregnancy.http://www.sciencedirect.com/science/article/pii/S0022227522001456S1PsphingosinepregnancypreeclampsiaPlGF |
| spellingShingle | Edward D. Johnstone Melissa Westwood Mark Dilworth Jonathan R. Wray Alexandra C. Kendall Anna Nicolaou Jenny E. Myers Plasma S1P and Sphingosine are not Different Prior to Pre-Eclampsia in Women at High Risk of Developing the Disease Journal of Lipid Research S1P sphingosine pregnancy preeclampsia PlGF |
| title | Plasma S1P and Sphingosine are not Different Prior to Pre-Eclampsia in Women at High Risk of Developing the Disease |
| title_full | Plasma S1P and Sphingosine are not Different Prior to Pre-Eclampsia in Women at High Risk of Developing the Disease |
| title_fullStr | Plasma S1P and Sphingosine are not Different Prior to Pre-Eclampsia in Women at High Risk of Developing the Disease |
| title_full_unstemmed | Plasma S1P and Sphingosine are not Different Prior to Pre-Eclampsia in Women at High Risk of Developing the Disease |
| title_short | Plasma S1P and Sphingosine are not Different Prior to Pre-Eclampsia in Women at High Risk of Developing the Disease |
| title_sort | plasma s1p and sphingosine are not different prior to pre eclampsia in women at high risk of developing the disease |
| topic | S1P sphingosine pregnancy preeclampsia PlGF |
| url | http://www.sciencedirect.com/science/article/pii/S0022227522001456 |
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