Neuroprotective Effects of Methyl 3,4-dihydroxybenzoate Against H2O2-Induced Apoptosis in RGC-5 Cells
Abstract.: In the present study, we investigated the protective effect of methyl 3,4-dihydroxybenzoate (MDHB) against H2O2-induced apoptosis in RGC-5 cells. The RGC-5 cells were cultured in plates for 24 h, which were then pretreated with dimethyl sulfoxide, different concentrations of MDHB, or prob...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2014-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319301513 |
| _version_ | 1811228571927052288 |
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| author | Xing Zhou Chao-Fen Su Zheng Zhang Chen-Yu Wang Jin-Qi Luo Xiao-Wen Zhou Liang Cai Li Yan Wei Zhang Huan-Min Luo |
| author_facet | Xing Zhou Chao-Fen Su Zheng Zhang Chen-Yu Wang Jin-Qi Luo Xiao-Wen Zhou Liang Cai Li Yan Wei Zhang Huan-Min Luo |
| author_sort | Xing Zhou |
| collection | DOAJ |
| description | Abstract.: In the present study, we investigated the protective effect of methyl 3,4-dihydroxybenzoate (MDHB) against H2O2-induced apoptosis in RGC-5 cells. The RGC-5 cells were cultured in plates for 24 h, which were then pretreated with dimethyl sulfoxide, different concentrations of MDHB, or probucol for 12 h prior to addition of 300 μM H2O2 for 24 h. The cell viability was detected by MTT assay. The rate of apoptosis, level of lipid peroxidation, and mitochondrial membrane potential (MMP) were detected by flow cytometry. Western blot analysis was also used to measure the expression level of Bcl-2, Bax, caspase 9, and caspase 3 proteins in H2O2-treated RGC-5 cells. Our study showed that the cell viability of RGC-5 cells significantly decreased after treatment with 300 μM H2O2 for 24 h, but MDHB (8, 16, 32 μM) increased RGC-5 cell survival, suppressed the rate of apoptosis, scavenged reactive oxygen species, and restored MMP. MDHB also obstructed H2O2-induced apoptosis by regulating the expression of Bcl-2 and Bax, as well as suppressing the activation of caspase 9 and caspase 3. Our results showed that MDHB is an effective neuroprotective compound that mitigates oxidative stress and inhibits apoptosis in RGC-5 cells. Keywords:: methyl 3,4-dihydroxybenzoate, retinal ganglion cell, neuroprotection, oxidative stress, apoptosis |
| first_indexed | 2024-04-12T09:59:53Z |
| format | Article |
| id | doaj.art-6c87aeaad21349eeab14f2954f517a08 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-04-12T09:59:53Z |
| publishDate | 2014-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-6c87aeaad21349eeab14f2954f517a082022-12-22T03:37:35ZengElsevierJournal of Pharmacological Sciences1347-86132014-01-0112515158Neuroprotective Effects of Methyl 3,4-dihydroxybenzoate Against H2O2-Induced Apoptosis in RGC-5 CellsXing Zhou0Chao-Fen Su1Zheng Zhang2Chen-Yu Wang3Jin-Qi Luo4Xiao-Wen Zhou5Liang Cai6Li Yan7Wei Zhang8Huan-Min Luo9Department of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China; The Ministry of Basic Medicine, Yichun Vocational and Technical College, Yichun 336000, ChinaDepartment of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, ChinaThe First Affiliated Hospital of Jinan University, Guangzhou 510632, ChinaDepartment of Clinical Medicine, School of Medicine, Jinan University, Guangzhou 510632, ChinaDepartment of Clinical Medicine, School of Medicine, Jinan University, Guangzhou 510632, ChinaPharmacy Department of GuangZhou Chest Hospital, Guangzhou 510632, ChinaDepartment of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, ChinaDepartment of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, ChinaDepartment of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, ChinaDepartment of Pharmacology, School of Medicine, Jinan University, Guangzhou 510632, China; Institute of Brain Sciences, Jinan University, Guangzhou 510632, China; The Joint Laboratory of Brain Function and Health, Jinan University and The University of Hong Kong, Jinan University, Guangzhou 510632, China; Corresponding author. tlhm@jnu.edu.cnAbstract.: In the present study, we investigated the protective effect of methyl 3,4-dihydroxybenzoate (MDHB) against H2O2-induced apoptosis in RGC-5 cells. The RGC-5 cells were cultured in plates for 24 h, which were then pretreated with dimethyl sulfoxide, different concentrations of MDHB, or probucol for 12 h prior to addition of 300 μM H2O2 for 24 h. The cell viability was detected by MTT assay. The rate of apoptosis, level of lipid peroxidation, and mitochondrial membrane potential (MMP) were detected by flow cytometry. Western blot analysis was also used to measure the expression level of Bcl-2, Bax, caspase 9, and caspase 3 proteins in H2O2-treated RGC-5 cells. Our study showed that the cell viability of RGC-5 cells significantly decreased after treatment with 300 μM H2O2 for 24 h, but MDHB (8, 16, 32 μM) increased RGC-5 cell survival, suppressed the rate of apoptosis, scavenged reactive oxygen species, and restored MMP. MDHB also obstructed H2O2-induced apoptosis by regulating the expression of Bcl-2 and Bax, as well as suppressing the activation of caspase 9 and caspase 3. Our results showed that MDHB is an effective neuroprotective compound that mitigates oxidative stress and inhibits apoptosis in RGC-5 cells. Keywords:: methyl 3,4-dihydroxybenzoate, retinal ganglion cell, neuroprotection, oxidative stress, apoptosishttp://www.sciencedirect.com/science/article/pii/S1347861319301513 |
| spellingShingle | Xing Zhou Chao-Fen Su Zheng Zhang Chen-Yu Wang Jin-Qi Luo Xiao-Wen Zhou Liang Cai Li Yan Wei Zhang Huan-Min Luo Neuroprotective Effects of Methyl 3,4-dihydroxybenzoate Against H2O2-Induced Apoptosis in RGC-5 Cells Journal of Pharmacological Sciences |
| title | Neuroprotective Effects of Methyl 3,4-dihydroxybenzoate Against H2O2-Induced Apoptosis in RGC-5 Cells |
| title_full | Neuroprotective Effects of Methyl 3,4-dihydroxybenzoate Against H2O2-Induced Apoptosis in RGC-5 Cells |
| title_fullStr | Neuroprotective Effects of Methyl 3,4-dihydroxybenzoate Against H2O2-Induced Apoptosis in RGC-5 Cells |
| title_full_unstemmed | Neuroprotective Effects of Methyl 3,4-dihydroxybenzoate Against H2O2-Induced Apoptosis in RGC-5 Cells |
| title_short | Neuroprotective Effects of Methyl 3,4-dihydroxybenzoate Against H2O2-Induced Apoptosis in RGC-5 Cells |
| title_sort | neuroprotective effects of methyl 3 4 dihydroxybenzoate against h2o2 induced apoptosis in rgc 5 cells |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319301513 |
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