Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative Analysis
<i>Linum usitatissimum</i> biosynthesizes lignans and neolignans that are diet and medicinally valuable metabolites. In recent years, zinc oxide nanoparticles (ZnONPs) have emerged as potential elicitors for the enhanced biosynthesis of commercial secondary metabolites. Herein, we invest...
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MDPI AG
2020-06-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/6/918 |
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| author | Afifa Zaeem Samantha Drouet Sumaira Anjum Razia Khurshid Muhammad Younas Jean Philippe Blondeau Duangjai Tungmunnithum Nathalie Giglioli-Guivarc’h Christophe Hano Bilal Haider Abbasi |
| author_facet | Afifa Zaeem Samantha Drouet Sumaira Anjum Razia Khurshid Muhammad Younas Jean Philippe Blondeau Duangjai Tungmunnithum Nathalie Giglioli-Guivarc’h Christophe Hano Bilal Haider Abbasi |
| author_sort | Afifa Zaeem |
| collection | DOAJ |
| description | <i>Linum usitatissimum</i> biosynthesizes lignans and neolignans that are diet and medicinally valuable metabolites. In recent years, zinc oxide nanoparticles (ZnONPs) have emerged as potential elicitors for the enhanced biosynthesis of commercial secondary metabolites. Herein, we investigated the influence of biogenic ZnONPs on both seedlings and stem-derived callus of <i>L. usitatissimum</i>. Seedlings of <i>L. usitatissimum</i> grown on Murashige and Skoog (MS) medium supplemented with ZnONPs (1–1000 mg/L) presented the highest antioxidant activity, total phenolic content, total flavonoid content, peroxidase and superoxide dismutase activities at 500 mg/L, while the maximum plantlet length was achieved with 10 mg/L. Likewise, the high-performance liquid chromatography (HPLC) analysis revealed the enhanced production of secoisolariciresinol diglucoside, lariciresinol diglucoside, dehydrodiconiferyl alcohol glucoside and guaiacylglycerol-β-coniferyl alcohol ether glucoside in the plantlets grown on the 500 mg/L ZnONPs. On the other hand, the stem explants were cultured on MS media comprising 1-naphthaleneacetic acid (1 mg/L) and ZnONPs (1–50 mg/L). The highest antioxidant and other activities with an enhanced rooting effect were noted in 25 mg/L ZnONP-treated callus. Similarly, the maximum metabolites were also accumulated in 25 mg/L ZnONP-treated callus. In both systems, the dose-dependent production of reactive oxygen species (ROS) was recorded, resulting in oxidative damage with a more pronounced toxic effect on in vitro cultures. Altogether, the results from this study constitute a first comprehensive view of the impact of ZnONPs on the oxidative stress and antioxidant responses in seedlings vs. in vitro cultures. |
| first_indexed | 2024-03-10T19:06:07Z |
| format | Article |
| id | doaj.art-6c87c3d4f9af48fbab7d4d60a7d2b3b4 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T19:06:07Z |
| publishDate | 2020-06-01 |
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| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-6c87c3d4f9af48fbab7d4d60a7d2b3b42023-11-20T04:09:38ZengMDPI AGBiomolecules2218-273X2020-06-0110691810.3390/biom10060918Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative AnalysisAfifa Zaeem0Samantha Drouet1Sumaira Anjum2Razia Khurshid3Muhammad Younas4Jean Philippe Blondeau5Duangjai Tungmunnithum6Nathalie Giglioli-Guivarc’h7Christophe Hano8Bilal Haider Abbasi9Department of Biotechnology, Quaid-i-Azam University, Islamabad 45320, PakistanLaboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRAE USC1328, University of Orleans, F28000 Chartres, FranceDepartment of Biotechnology, Kinnaird College for Women, Lahore 54000, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad 45320, PakistanDepartment of Biotechnology, Quaid-i-Azam University, Islamabad 45320, PakistanConditions Extrêmes et Matériaux, Haute Température et Irradiation (CEMHTI) CNRS UPR3079, 1D Avenue de la Recherche Scientifique, 45071 Orléans, FranceLaboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRAE USC1328, University of Orleans, F28000 Chartres, FranceBiomolecules et Biotechnologies Vegetales, EA2106, Universite Francois-Rabelais de Tours, 37000 Tours, FranceLaboratoire de Biologie des Ligneux et des Grandes Cultures (LBLGC), INRAE USC1328, University of Orleans, F28000 Chartres, FranceDepartment of Biotechnology, Quaid-i-Azam University, Islamabad 45320, Pakistan<i>Linum usitatissimum</i> biosynthesizes lignans and neolignans that are diet and medicinally valuable metabolites. In recent years, zinc oxide nanoparticles (ZnONPs) have emerged as potential elicitors for the enhanced biosynthesis of commercial secondary metabolites. Herein, we investigated the influence of biogenic ZnONPs on both seedlings and stem-derived callus of <i>L. usitatissimum</i>. Seedlings of <i>L. usitatissimum</i> grown on Murashige and Skoog (MS) medium supplemented with ZnONPs (1–1000 mg/L) presented the highest antioxidant activity, total phenolic content, total flavonoid content, peroxidase and superoxide dismutase activities at 500 mg/L, while the maximum plantlet length was achieved with 10 mg/L. Likewise, the high-performance liquid chromatography (HPLC) analysis revealed the enhanced production of secoisolariciresinol diglucoside, lariciresinol diglucoside, dehydrodiconiferyl alcohol glucoside and guaiacylglycerol-β-coniferyl alcohol ether glucoside in the plantlets grown on the 500 mg/L ZnONPs. On the other hand, the stem explants were cultured on MS media comprising 1-naphthaleneacetic acid (1 mg/L) and ZnONPs (1–50 mg/L). The highest antioxidant and other activities with an enhanced rooting effect were noted in 25 mg/L ZnONP-treated callus. Similarly, the maximum metabolites were also accumulated in 25 mg/L ZnONP-treated callus. In both systems, the dose-dependent production of reactive oxygen species (ROS) was recorded, resulting in oxidative damage with a more pronounced toxic effect on in vitro cultures. Altogether, the results from this study constitute a first comprehensive view of the impact of ZnONPs on the oxidative stress and antioxidant responses in seedlings vs. in vitro cultures.https://www.mdpi.com/2218-273X/10/6/918<i>Linum usitatissimum</i> L.zinc oxide nanoparticlesantioxidantsperoxidasesuperoxide dismutasephenolics |
| spellingShingle | Afifa Zaeem Samantha Drouet Sumaira Anjum Razia Khurshid Muhammad Younas Jean Philippe Blondeau Duangjai Tungmunnithum Nathalie Giglioli-Guivarc’h Christophe Hano Bilal Haider Abbasi Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative Analysis Biomolecules <i>Linum usitatissimum</i> L. zinc oxide nanoparticles antioxidants peroxidase superoxide dismutase phenolics |
| title | Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative Analysis |
| title_full | Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative Analysis |
| title_fullStr | Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative Analysis |
| title_full_unstemmed | Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative Analysis |
| title_short | Effects of Biogenic Zinc Oxide Nanoparticles on Growth and Oxidative Stress Response in Flax Seedlings vs. In Vitro Cultures: A Comparative Analysis |
| title_sort | effects of biogenic zinc oxide nanoparticles on growth and oxidative stress response in flax seedlings vs in vitro cultures a comparative analysis |
| topic | <i>Linum usitatissimum</i> L. zinc oxide nanoparticles antioxidants peroxidase superoxide dismutase phenolics |
| url | https://www.mdpi.com/2218-273X/10/6/918 |
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