<i>FvMYB44</i>, a Strawberry <i>R2R3-MYB</i> Transcription Factor, Improved Salt and Cold Stress Tolerance in Transgenic <i>Arabidopsis</i>
MYB (v-MYB avian myeloblastosis viral oncogene homolog) transcription factor (TF) family has numerous members with complex and diverse functions, which perform an integral role in regulating the plant’s response to adversity. This study used cloning to obtain a novel MYB TF gene from the diploid str...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/4/1051 |
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| author | Wenhui Li Yangfan Wei Lihua Zhang Yu Wang Penghui Song Xingguo Li Deguo Han |
| author_facet | Wenhui Li Yangfan Wei Lihua Zhang Yu Wang Penghui Song Xingguo Li Deguo Han |
| author_sort | Wenhui Li |
| collection | DOAJ |
| description | MYB (v-MYB avian myeloblastosis viral oncogene homolog) transcription factor (TF) family has numerous members with complex and diverse functions, which perform an integral role in regulating the plant’s response to adversity. This study used cloning to obtain a novel MYB TF gene from the diploid strawberry <i>Fragaria vesca</i>, which was given the designation <i>FvMYB44</i>. Subcellular localization results showed that the protein of FvMYB44 was a nuclear localization protein. The resistance of <i>Arabidopsis thaliana</i> to salt and low temperature stresses was greatly enhanced by the overexpression of <i>FvMYB44</i>. When subjected to salt and temperature stress, transgenic plants showed higher proline and chlorophyll concentrations and higher superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities than wild-type (WT) and unloaded line (UL) of <i>A. thaliana</i>. In contrast, WT and UL lines had higher malondialdehyde (MDA) content and reactive oxygen species ROS (O<sub>2</sub><sup>−</sup> and H<sub>2</sub>O<sub>2</sub>) content. These findings suggest that <i>FvMYB44</i> may perform a role in controlling the response of <i>A. thaliana</i> to cold and salt stress. |
| first_indexed | 2024-03-11T05:19:56Z |
| format | Article |
| id | doaj.art-6c88164f34a94d138d5a8a80dd80ee8b |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T05:19:56Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-6c88164f34a94d138d5a8a80dd80ee8b2023-11-17T17:56:53ZengMDPI AGAgronomy2073-43952023-04-01134105110.3390/agronomy13041051<i>FvMYB44</i>, a Strawberry <i>R2R3-MYB</i> Transcription Factor, Improved Salt and Cold Stress Tolerance in Transgenic <i>Arabidopsis</i>Wenhui Li0Yangfan Wei1Lihua Zhang2Yu Wang3Penghui Song4Xingguo Li5Deguo Han6Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaHorticulture Branch of Heilongjiang Academy of Agricultural Sciences, Harbin 150040, ChinaInstitute of Rural Revitalization Science and Technology, Heilongjiang Academy of Agricultural Sciences, Harbin 150028, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northeast Region), Ministry of Agriculture and Rural Affairs, National-Local Joint Engineering Research Center for Development and Utilization of Small Fruits in Cold Regions, College of Horticulture & Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaMYB (v-MYB avian myeloblastosis viral oncogene homolog) transcription factor (TF) family has numerous members with complex and diverse functions, which perform an integral role in regulating the plant’s response to adversity. This study used cloning to obtain a novel MYB TF gene from the diploid strawberry <i>Fragaria vesca</i>, which was given the designation <i>FvMYB44</i>. Subcellular localization results showed that the protein of FvMYB44 was a nuclear localization protein. The resistance of <i>Arabidopsis thaliana</i> to salt and low temperature stresses was greatly enhanced by the overexpression of <i>FvMYB44</i>. When subjected to salt and temperature stress, transgenic plants showed higher proline and chlorophyll concentrations and higher superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities than wild-type (WT) and unloaded line (UL) of <i>A. thaliana</i>. In contrast, WT and UL lines had higher malondialdehyde (MDA) content and reactive oxygen species ROS (O<sub>2</sub><sup>−</sup> and H<sub>2</sub>O<sub>2</sub>) content. These findings suggest that <i>FvMYB44</i> may perform a role in controlling the response of <i>A. thaliana</i> to cold and salt stress.https://www.mdpi.com/2073-4395/13/4/1051<i>Fragaria vesca</i><i>FvMYB44</i>salt stresscold stress<i>Arabidopsis thaliana</i> |
| spellingShingle | Wenhui Li Yangfan Wei Lihua Zhang Yu Wang Penghui Song Xingguo Li Deguo Han <i>FvMYB44</i>, a Strawberry <i>R2R3-MYB</i> Transcription Factor, Improved Salt and Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> Agronomy <i>Fragaria vesca</i> <i>FvMYB44</i> salt stress cold stress <i>Arabidopsis thaliana</i> |
| title | <i>FvMYB44</i>, a Strawberry <i>R2R3-MYB</i> Transcription Factor, Improved Salt and Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> |
| title_full | <i>FvMYB44</i>, a Strawberry <i>R2R3-MYB</i> Transcription Factor, Improved Salt and Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> |
| title_fullStr | <i>FvMYB44</i>, a Strawberry <i>R2R3-MYB</i> Transcription Factor, Improved Salt and Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> |
| title_full_unstemmed | <i>FvMYB44</i>, a Strawberry <i>R2R3-MYB</i> Transcription Factor, Improved Salt and Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> |
| title_short | <i>FvMYB44</i>, a Strawberry <i>R2R3-MYB</i> Transcription Factor, Improved Salt and Cold Stress Tolerance in Transgenic <i>Arabidopsis</i> |
| title_sort | i fvmyb44 i a strawberry i r2r3 myb i transcription factor improved salt and cold stress tolerance in transgenic i arabidopsis i |
| topic | <i>Fragaria vesca</i> <i>FvMYB44</i> salt stress cold stress <i>Arabidopsis thaliana</i> |
| url | https://www.mdpi.com/2073-4395/13/4/1051 |
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