Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture Activities
Rising trends in fish filleting are increasing the amount of processing by-products, such as skins of turbot, a flatfish of high commercial value. In line with circular economy principles, we propose the valorization of turbot skins through a two-step process: initial gelatin extraction described fo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/9/491 |
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| author | Jesus Valcarcel Javier Fraguas Carolina Hermida-Merino Daniel Hermida-Merino Manuel M. Piñeiro José Antonio Vázquez |
| author_facet | Jesus Valcarcel Javier Fraguas Carolina Hermida-Merino Daniel Hermida-Merino Manuel M. Piñeiro José Antonio Vázquez |
| author_sort | Jesus Valcarcel |
| collection | DOAJ |
| description | Rising trends in fish filleting are increasing the amount of processing by-products, such as skins of turbot, a flatfish of high commercial value. In line with circular economy principles, we propose the valorization of turbot skins through a two-step process: initial gelatin extraction described for the first time in turbot, followed by hydrolysis of the remaining solids to produce collagen hydrolysates. We assayed several methods for gelatin extraction, finding differences in gelatin properties depending on chemical treatment and temperature. Of all methods, the application of NaOH, sulfuric, and citric acids at 22 °C results in the highest gel strength (177 g), storage and loss moduli, and gel stability. We found no relation between mechanical properties and content of pyrrolidine amino acids, but the best performing gelatin displays higher structural integrity, with less than 30% of the material below 100 kDa. Collagen hydrolysis was more efficient with papain than alcalase, leading to a greater reduction in Mw of the hydrolysates, which contain a higher proportion of essential amino acids than gelatin and show high in vitro anti-hypertensive activity. These results highlight the suitability of turbot skin by-products as a source of gelatin and the potential of collagen hydrolysates as a functional food and feed ingredient. |
| first_indexed | 2024-03-10T07:29:58Z |
| format | Article |
| id | doaj.art-6c8a8e38e6c344e19d257b0eb520f0ed |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T07:29:58Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-6c8a8e38e6c344e19d257b0eb520f0ed2023-11-22T13:58:36ZengMDPI AGMarine Drugs1660-33972021-08-0119949110.3390/md19090491Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture ActivitiesJesus Valcarcel0Javier Fraguas1Carolina Hermida-Merino2Daniel Hermida-Merino3Manuel M. Piñeiro4José Antonio Vázquez5Group of Recycling and Valorization of Waste Materials (REVAL), Marine Research Institute (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo, SpainGroup of Recycling and Valorization of Waste Materials (REVAL), Marine Research Institute (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo, SpainCentro de Investigaciones Biomédicas (CINBIO), Departamento de Física Aplicada, Facultad de Ciencias, Universidade de Vigo, 36310 Vigo, SpainNetherlands Organization for Scientific Research (NWO), DUBBLE@ESRF, BP220, F38043 Grenoble, FranceCentro de Investigaciones Biomédicas (CINBIO), Departamento de Física Aplicada, Facultad de Ciencias, Universidade de Vigo, 36310 Vigo, SpainGroup of Recycling and Valorization of Waste Materials (REVAL), Marine Research Institute (IIM-CSIC), Eduardo Cabello 6, 36208 Vigo, SpainRising trends in fish filleting are increasing the amount of processing by-products, such as skins of turbot, a flatfish of high commercial value. In line with circular economy principles, we propose the valorization of turbot skins through a two-step process: initial gelatin extraction described for the first time in turbot, followed by hydrolysis of the remaining solids to produce collagen hydrolysates. We assayed several methods for gelatin extraction, finding differences in gelatin properties depending on chemical treatment and temperature. Of all methods, the application of NaOH, sulfuric, and citric acids at 22 °C results in the highest gel strength (177 g), storage and loss moduli, and gel stability. We found no relation between mechanical properties and content of pyrrolidine amino acids, but the best performing gelatin displays higher structural integrity, with less than 30% of the material below 100 kDa. Collagen hydrolysis was more efficient with papain than alcalase, leading to a greater reduction in Mw of the hydrolysates, which contain a higher proportion of essential amino acids than gelatin and show high in vitro anti-hypertensive activity. These results highlight the suitability of turbot skin by-products as a source of gelatin and the potential of collagen hydrolysates as a functional food and feed ingredient.https://www.mdpi.com/1660-3397/19/9/491turbot gelatinaquaculture by-products valorizationmarine biomaterials |
| spellingShingle | Jesus Valcarcel Javier Fraguas Carolina Hermida-Merino Daniel Hermida-Merino Manuel M. Piñeiro José Antonio Vázquez Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture Activities Marine Drugs turbot gelatin aquaculture by-products valorization marine biomaterials |
| title | Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture Activities |
| title_full | Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture Activities |
| title_fullStr | Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture Activities |
| title_full_unstemmed | Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture Activities |
| title_short | Production and Physicochemical Characterization of Gelatin and Collagen Hydrolysates from Turbot Skin Waste Generated by Aquaculture Activities |
| title_sort | production and physicochemical characterization of gelatin and collagen hydrolysates from turbot skin waste generated by aquaculture activities |
| topic | turbot gelatin aquaculture by-products valorization marine biomaterials |
| url | https://www.mdpi.com/1660-3397/19/9/491 |
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