Odontogenic Differentiation-Induced Tooth Regeneration by <i>Psoralea corylifolia</i> L.
<i>Psoralea corylifolia</i> L. (<i>P. corylifolia</i>) has been used as an oriental phytomedicine to treat coldness of hands and feet in bone marrow injury. Hydroxyapatite is usually used for tooth regeneration. In this study, the role of <i>P. corylifolia</i> and...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-05-01
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| Series: | Current Issues in Molecular Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1467-3045/44/5/156 |
| Summary: | <i>Psoralea corylifolia</i> L. (<i>P. corylifolia</i>) has been used as an oriental phytomedicine to treat coldness of hands and feet in bone marrow injury. Hydroxyapatite is usually used for tooth regeneration. In this study, the role of <i>P. corylifolia</i> and bakuchiol, a compound originated from <i>P. corylifolia</i> as differentiation-inducing substances for tooth regeneration, was determined by monitoring odontogenic differentiation in human dental pulp stem cells (hDPSCs). We confirmed that <i>P. corylifolia</i> extracts and bakuchiol increased the odontogenic differentiation of hDPSCs. In addition, the expression of the odontogenic differentiation marker genes alkaline phosphatase (APL), Runt-related transcription factor 2 (RUNX-2), osteocalcin (OC), and dentin matrix acidic phosphoprotein-1 (DMP-1) was proved by real-time polymerase chain reaction, and protein expression of dentin matrix acidic phosphoprotein-1 (DMP-1) and dentin sialophosphoprotein (DSPP) was proved by western blotting. Further, by confirming the increase in small mothers against decapentaplegia (SMAD) 1/5/8 phosphorylation, the SMAD signaling pathway was found to increase the differentiation of odontoblasts. This study confirmed that <i>P. corylifolia</i> L. extracts and bakuchiol alone promote odontogenic differentiation in hDPSCs. These results suggest that bakuchiol from <i>P. corylifolia</i> is responsible for odontogenic differentiation, and they encourage future in vivo studies on dentin regeneration. |
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| ISSN: | 1467-3037 1467-3045 |