A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis
Abstract The importance of the microbiome for bovine udder health is not well explored and most of the knowledge originates from research on mastitis. Better understanding of the microbial diversity inside the healthy udder of lactating cows might help to reduce mastitis, use of antibiotics and impr...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2020-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-77054-6 |
| _version_ | 1819200285490282496 |
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| author | Davide Porcellato Roger Meisal Alberto Bombelli Judith A. Narvhus |
| author_facet | Davide Porcellato Roger Meisal Alberto Bombelli Judith A. Narvhus |
| author_sort | Davide Porcellato |
| collection | DOAJ |
| description | Abstract The importance of the microbiome for bovine udder health is not well explored and most of the knowledge originates from research on mastitis. Better understanding of the microbial diversity inside the healthy udder of lactating cows might help to reduce mastitis, use of antibiotics and improve animal welfare. In this study, we investigated the microbial diversity of over 400 quarter milk samples from 60 cows sampled from two farms and on two different occasions during the same lactation period. Microbiota analysis was performed using amplicon sequencing of the 16S rRNA gene and over 1000 isolates were identified using MALDI-TOF MS. We detected a high abundance of two bacterial families, Corynebacteriaceae and Staphylococcaceae, which accounted for almost 50% of the udder microbiota of healthy cows and were detected in all the cow udders and in more than 98% of quarter milk samples. A strong negative correlation between these bacterial families was detected indicating a possible competition. The overall composition of the udder microbiota was highly diverse and significantly different between cows and between quarter milk samples from the same cow. Furthermore, we introduced a novel definition of a dysbiotic quarter at individual cow level, by analyzing the milk microbiota, and a high frequency of dysbiotic quarter samples were detected distributed among the farms and the samples. These results emphasize the importance of deepening the studies of the bovine udder microbiome to elucidate its role in udder health. |
| first_indexed | 2024-12-23T03:29:48Z |
| format | Article |
| id | doaj.art-6c902962838341fdae3e7f174147b602 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-23T03:29:48Z |
| publishDate | 2020-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-6c902962838341fdae3e7f174147b6022022-12-21T18:01:44ZengNature PortfolioScientific Reports2045-23222020-12-0110111410.1038/s41598-020-77054-6A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosisDavide Porcellato0Roger Meisal1Alberto Bombelli2Judith A. Narvhus3Faculty of Chemistry, Biotechnology and Food Science, The Norwegian University of Life SciencesFaculty of Chemistry, Biotechnology and Food Science, The Norwegian University of Life SciencesFaculty of Chemistry, Biotechnology and Food Science, The Norwegian University of Life SciencesFaculty of Chemistry, Biotechnology and Food Science, The Norwegian University of Life SciencesAbstract The importance of the microbiome for bovine udder health is not well explored and most of the knowledge originates from research on mastitis. Better understanding of the microbial diversity inside the healthy udder of lactating cows might help to reduce mastitis, use of antibiotics and improve animal welfare. In this study, we investigated the microbial diversity of over 400 quarter milk samples from 60 cows sampled from two farms and on two different occasions during the same lactation period. Microbiota analysis was performed using amplicon sequencing of the 16S rRNA gene and over 1000 isolates were identified using MALDI-TOF MS. We detected a high abundance of two bacterial families, Corynebacteriaceae and Staphylococcaceae, which accounted for almost 50% of the udder microbiota of healthy cows and were detected in all the cow udders and in more than 98% of quarter milk samples. A strong negative correlation between these bacterial families was detected indicating a possible competition. The overall composition of the udder microbiota was highly diverse and significantly different between cows and between quarter milk samples from the same cow. Furthermore, we introduced a novel definition of a dysbiotic quarter at individual cow level, by analyzing the milk microbiota, and a high frequency of dysbiotic quarter samples were detected distributed among the farms and the samples. These results emphasize the importance of deepening the studies of the bovine udder microbiome to elucidate its role in udder health.https://doi.org/10.1038/s41598-020-77054-6 |
| spellingShingle | Davide Porcellato Roger Meisal Alberto Bombelli Judith A. Narvhus A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis Scientific Reports |
| title | A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis |
| title_full | A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis |
| title_fullStr | A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis |
| title_full_unstemmed | A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis |
| title_short | A core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis |
| title_sort | core microbiota dominates a rich microbial diversity in the bovine udder and may indicate presence of dysbiosis |
| url | https://doi.org/10.1038/s41598-020-77054-6 |
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