In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses
Piccolo is the largest known cytomatrix protein at active zones of chemical synapses. A growing number of studies on conventional chemical synapses assign Piccolo a role in the recruitment and integration of molecules relevant for both endo- and exocytosis of synaptic vesicles, the dynamic assembly...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-09-01
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| Series: | Frontiers in Cellular Neuroscience |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00259/full |
| _version_ | 1818265353995681792 |
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| author | Hanna eRegus-Leidig Michaela eFuchs Martina eLöhner Sarah eLeist Sarah eLeist Sergio eLeal-Ortiz Vince eChiodo William eHauswirth Craig eGarner Craig eGarner Johann Helmut Brandstätter |
| author_facet | Hanna eRegus-Leidig Michaela eFuchs Martina eLöhner Sarah eLeist Sarah eLeist Sergio eLeal-Ortiz Vince eChiodo William eHauswirth Craig eGarner Craig eGarner Johann Helmut Brandstätter |
| author_sort | Hanna eRegus-Leidig |
| collection | DOAJ |
| description | Piccolo is the largest known cytomatrix protein at active zones of chemical synapses. A growing number of studies on conventional chemical synapses assign Piccolo a role in the recruitment and integration of molecules relevant for both endo- and exocytosis of synaptic vesicles, the dynamic assembly of presynaptic F-actin, as well as the proteostasis of presynaptic proteins, yet a direct function in the structural organization of the active zone has not been uncovered in part due to the expression of multiple alternatively spliced isoforms. We recently identified Piccolino, a Piccolo splice variant specifically expressed in sensory ribbon synapses of the eye and ear. Here we down regulated Piccolino in vivo via an adeno-associated virus-based RNA interference approach and explored the impact on the presynaptic structure of mouse photoreceptor ribbon synapses. Detailed immunocytochemical light and electron microscopical analysis of Piccolino knockdown in photoreceptors revealed a hitherto undescribed photoreceptor ribbon synaptic phenotype with striking morphological changes of synaptic ribbon ultrastructure. |
| first_indexed | 2024-12-12T19:49:28Z |
| format | Article |
| id | doaj.art-6c926733565e4683a84f19ce57fc6177 |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-12-12T19:49:28Z |
| publishDate | 2014-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-6c926733565e4683a84f19ce57fc61772022-12-22T00:14:00ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022014-09-01810.3389/fncel.2014.00259102230In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapsesHanna eRegus-Leidig0Michaela eFuchs1Martina eLöhner2Sarah eLeist3Sarah eLeist4Sergio eLeal-Ortiz5Vince eChiodo6William eHauswirth7Craig eGarner8Craig eGarner9Johann Helmut Brandstätter10Friedrich-Alexander-University of Erlangen-NurembergFriedrich-Alexander-University of Erlangen-NurembergFriedrich-Alexander-University of Erlangen-NurembergFriedrich-Alexander-University of Erlangen-NurembergHelmholtz Centre for Infection ResearchStanford UniversityCollege of Medicine, University of FloridaCollege of Medicine, University of FloridaStanford UniversityDeutsches Zentrum für Neurodegenerative Erkrankungen, CharitéFriedrich-Alexander-University of Erlangen-NurembergPiccolo is the largest known cytomatrix protein at active zones of chemical synapses. A growing number of studies on conventional chemical synapses assign Piccolo a role in the recruitment and integration of molecules relevant for both endo- and exocytosis of synaptic vesicles, the dynamic assembly of presynaptic F-actin, as well as the proteostasis of presynaptic proteins, yet a direct function in the structural organization of the active zone has not been uncovered in part due to the expression of multiple alternatively spliced isoforms. We recently identified Piccolino, a Piccolo splice variant specifically expressed in sensory ribbon synapses of the eye and ear. Here we down regulated Piccolino in vivo via an adeno-associated virus-based RNA interference approach and explored the impact on the presynaptic structure of mouse photoreceptor ribbon synapses. Detailed immunocytochemical light and electron microscopical analysis of Piccolino knockdown in photoreceptors revealed a hitherto undescribed photoreceptor ribbon synaptic phenotype with striking morphological changes of synaptic ribbon ultrastructure.http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00259/fullRetinaactive Zoneribbon synapseRIBEYEPiccolinoPiccolo |
| spellingShingle | Hanna eRegus-Leidig Michaela eFuchs Martina eLöhner Sarah eLeist Sarah eLeist Sergio eLeal-Ortiz Vince eChiodo William eHauswirth Craig eGarner Craig eGarner Johann Helmut Brandstätter In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses Frontiers in Cellular Neuroscience Retina active Zone ribbon synapse RIBEYE Piccolino Piccolo |
| title | In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses |
| title_full | In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses |
| title_fullStr | In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses |
| title_full_unstemmed | In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses |
| title_short | In vivo knockdown of Piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses |
| title_sort | in vivo knockdown of piccolino disrupts presynaptic ribbon morphology in mouse photoreceptor synapses |
| topic | Retina active Zone ribbon synapse RIBEYE Piccolino Piccolo |
| url | http://journal.frontiersin.org/Journal/10.3389/fncel.2014.00259/full |
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