Molecules produced by probiotics prevent enteric colibacillosis in pigs
Abstract Background With the advent of antimicrobial resistance in animal pathogens, novel methods to combat infectious diseases are being sought. Among these, probiotics have been proposed as a means of promoting animal health but problems with their use has been reported. Research has demonstrated...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-11-01
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| Series: | BMC Veterinary Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12917-017-1246-6 |
| _version_ | 1818501485507379200 |
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| author | Ricardo Nordeste Akalate Tessema Sapana Sharma Zlatko Kovač Chuan Wang Rocio Morales Mansel William Griffiths |
| author_facet | Ricardo Nordeste Akalate Tessema Sapana Sharma Zlatko Kovač Chuan Wang Rocio Morales Mansel William Griffiths |
| author_sort | Ricardo Nordeste |
| collection | DOAJ |
| description | Abstract Background With the advent of antimicrobial resistance in animal pathogens, novel methods to combat infectious diseases are being sought. Among these, probiotics have been proposed as a means of promoting animal health but problems with their use has been reported. Research has demonstrated that bioactive molecules produced during the growth of certain probiotics interfere with bacterial cell-to-cell communication, which consequently results in an attenuation of virulence in a number of pathogens, including E. coli. The objective of this study was to determine the efficacy of the bioactive molecules, termed proteobiotics, produced by Lactobacillus acidophilus in preventing enterotoxigenic E, coli (ETEC) infection in pigs, which is the etiological agent for enteric colibacillosis, a common disease of nursing and young pigs. Results To achieve this, piglets were fed a preparation of the bioactive at four levels: 0, 0.5×, 1.0× and 2.0× for 7 days prior to challenge with E. coli K88. There were 36 pigs (18 gilts and 18 barrows) per treatment, resulting in 144 piglets in total for the study. Each pen had 6 piglets (3 gilts and 3 barrows). Only piglets with no physical abnormality or conditions were used in the trial and intact male piglets and ridglings were excluded. The bioactive continued to be fed to the pigs post-challenge. Based of fecal and demeanour scores, pigs fed the low and high dose of the proteobiotic were significanlty less likely to show symptoms of illness than pigs fed no bioactive. While not being significant, the weight gain of pigs given the proteobiotics was improved. At day 4 following challenge, almost 50% of piglets that did not receive the proteobiotic were shedding ETEC in their feces, compared with about 15% of animals receiving the supplement. There was also an indication that the proteobiotics reduced colonization of the ileum by E. coli K88 and improved gut health. Conclusion This study indicates that the bioactive molecules produced by L. acidophilus reduces incidence of enteric colibacillosis in pigs and their use on farms would help to reduce antibiotic use. |
| first_indexed | 2024-12-10T20:56:53Z |
| format | Article |
| id | doaj.art-6c94eaa86aa148e49b946588d5df2b70 |
| institution | Directory Open Access Journal |
| issn | 1746-6148 |
| language | English |
| last_indexed | 2024-12-10T20:56:53Z |
| publishDate | 2017-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj.art-6c94eaa86aa148e49b946588d5df2b702022-12-22T01:33:56ZengBMCBMC Veterinary Research1746-61482017-11-0113111210.1186/s12917-017-1246-6Molecules produced by probiotics prevent enteric colibacillosis in pigsRicardo Nordeste0Akalate Tessema1Sapana Sharma2Zlatko Kovač3Chuan Wang4Rocio Morales5Mansel William Griffiths6MicroSintesis Inc., Duffy Research CentreCanadian Research Institute for Food Safety, University of GuelphCanadian Research Institute for Food Safety, University of GuelphDepartment of Veterinary Clinical and Animal Sciences, Faculty of Health and Medical Sciences, University of CopenhagenCanadian Research Institute for Food Safety, University of GuelphCanadian Research Institute for Food Safety, University of GuelphCanadian Research Institute for Food Safety, University of GuelphAbstract Background With the advent of antimicrobial resistance in animal pathogens, novel methods to combat infectious diseases are being sought. Among these, probiotics have been proposed as a means of promoting animal health but problems with their use has been reported. Research has demonstrated that bioactive molecules produced during the growth of certain probiotics interfere with bacterial cell-to-cell communication, which consequently results in an attenuation of virulence in a number of pathogens, including E. coli. The objective of this study was to determine the efficacy of the bioactive molecules, termed proteobiotics, produced by Lactobacillus acidophilus in preventing enterotoxigenic E, coli (ETEC) infection in pigs, which is the etiological agent for enteric colibacillosis, a common disease of nursing and young pigs. Results To achieve this, piglets were fed a preparation of the bioactive at four levels: 0, 0.5×, 1.0× and 2.0× for 7 days prior to challenge with E. coli K88. There were 36 pigs (18 gilts and 18 barrows) per treatment, resulting in 144 piglets in total for the study. Each pen had 6 piglets (3 gilts and 3 barrows). Only piglets with no physical abnormality or conditions were used in the trial and intact male piglets and ridglings were excluded. The bioactive continued to be fed to the pigs post-challenge. Based of fecal and demeanour scores, pigs fed the low and high dose of the proteobiotic were significanlty less likely to show symptoms of illness than pigs fed no bioactive. While not being significant, the weight gain of pigs given the proteobiotics was improved. At day 4 following challenge, almost 50% of piglets that did not receive the proteobiotic were shedding ETEC in their feces, compared with about 15% of animals receiving the supplement. There was also an indication that the proteobiotics reduced colonization of the ileum by E. coli K88 and improved gut health. Conclusion This study indicates that the bioactive molecules produced by L. acidophilus reduces incidence of enteric colibacillosis in pigs and their use on farms would help to reduce antibiotic use.http://link.springer.com/article/10.1186/s12917-017-1246-6E. coliProbioticsBioactive moleculesProteobioticsPigsEnteric colibacillosis |
| spellingShingle | Ricardo Nordeste Akalate Tessema Sapana Sharma Zlatko Kovač Chuan Wang Rocio Morales Mansel William Griffiths Molecules produced by probiotics prevent enteric colibacillosis in pigs BMC Veterinary Research E. coli Probiotics Bioactive molecules Proteobiotics Pigs Enteric colibacillosis |
| title | Molecules produced by probiotics prevent enteric colibacillosis in pigs |
| title_full | Molecules produced by probiotics prevent enteric colibacillosis in pigs |
| title_fullStr | Molecules produced by probiotics prevent enteric colibacillosis in pigs |
| title_full_unstemmed | Molecules produced by probiotics prevent enteric colibacillosis in pigs |
| title_short | Molecules produced by probiotics prevent enteric colibacillosis in pigs |
| title_sort | molecules produced by probiotics prevent enteric colibacillosis in pigs |
| topic | E. coli Probiotics Bioactive molecules Proteobiotics Pigs Enteric colibacillosis |
| url | http://link.springer.com/article/10.1186/s12917-017-1246-6 |
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