STUDIES OF MAGNESIUM AND PHOSPHORUS COMBINED MEDICATION BASED ON CASEIN
Aim. The Department of Biochemistry and Physiology of Animals, named after Academician Guly NUBIP of Ukraine, developed magnesium and phosphorus combined medication based on casein. Our aim was to test its bioavailability based on the ability to be hydrolyzed by a mixture of pancreatic digestive enz...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2021-10-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/5_2021/palonko_5_2021.pdf |
| _version_ | 1797766857404448768 |
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| author | Palonko R.I. Pavlyuk O.V., Arnauta O.V. Kalachniuk L.H. |
| author_facet | Palonko R.I. Pavlyuk O.V., Arnauta O.V. Kalachniuk L.H. |
| author_sort | Palonko R.I. |
| collection | DOAJ |
| description | Aim. The Department of Biochemistry and Physiology of Animals, named after Academician Guly NUBIP of Ukraine, developed magnesium and phosphorus combined medication based on casein. Our aim was to test its bioavailability based on the ability to be hydrolyzed by a mixture of pancreatic digestive enzymes trypsin and chymotrypsin, also check the absence of cytotoxic effects on cell cultures.
Methods. To assess bioavailability, we used hydrolysis of the medication with a mixture of trypsin and chymotrypsin, followed by detection of hydrolysis products by polyacrylamide gel electrophoresis. A standard MTT-test performed on both MT-4 and Namalva cell lines was used to assess cytotoxic effects.
Results. Based on electrophoresis data, it was found that despite chemical modifications of the natural casein, the medication based on it is characterized by a high ability to hydrolyze by digestive enzymes under the same conditions as casein. Also, an MTT-test demonstrates that the medication has no cytotoxic properties against cell lines MT-4 and Namalva.
Conclusions. Since the negative effects of the drug associated with its digestibility and toxicity have not been observed, it is recommended to continue the study of its effects on living organisms. |
| first_indexed | 2024-03-12T20:31:19Z |
| format | Article |
| id | doaj.art-6c952f75c5a544178c08857100ad5a76 |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T20:31:19Z |
| publishDate | 2021-10-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-6c952f75c5a544178c08857100ad5a762023-08-02T00:03:03ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2021-10-01145566210.15407/biotech14.05.056STUDIES OF MAGNESIUM AND PHOSPHORUS COMBINED MEDICATION BASED ON CASEINPalonko R.I.0Pavlyuk O.V.,1Arnauta O.V.2 Kalachniuk L.H.3National University of Life and Environmental Sciences of UkraineNational University of Life and Environmental Sciences of UkraineNational University of Life and Environmental Sciences of UkraineNational University of Life and Environmental Sciences of UkraineAim. The Department of Biochemistry and Physiology of Animals, named after Academician Guly NUBIP of Ukraine, developed magnesium and phosphorus combined medication based on casein. Our aim was to test its bioavailability based on the ability to be hydrolyzed by a mixture of pancreatic digestive enzymes trypsin and chymotrypsin, also check the absence of cytotoxic effects on cell cultures. Methods. To assess bioavailability, we used hydrolysis of the medication with a mixture of trypsin and chymotrypsin, followed by detection of hydrolysis products by polyacrylamide gel electrophoresis. A standard MTT-test performed on both MT-4 and Namalva cell lines was used to assess cytotoxic effects. Results. Based on electrophoresis data, it was found that despite chemical modifications of the natural casein, the medication based on it is characterized by a high ability to hydrolyze by digestive enzymes under the same conditions as casein. Also, an MTT-test demonstrates that the medication has no cytotoxic properties against cell lines MT-4 and Namalva. Conclusions. Since the negative effects of the drug associated with its digestibility and toxicity have not been observed, it is recommended to continue the study of its effects on living organisms.http://biotechnology.kiev.ua/images/5_2021/palonko_5_2021.pdfkey words: magnesiumphosphoruscaseinchelatein vitrohydrolysiscell culturecytotoxicitymtt reagentnadh (nicotinamide adenine dinucleotide). |
| spellingShingle | Palonko R.I. Pavlyuk O.V., Arnauta O.V. Kalachniuk L.H. STUDIES OF MAGNESIUM AND PHOSPHORUS COMBINED MEDICATION BASED ON CASEIN Biotechnologia Acta key words: magnesium phosphorus casein chelate in vitro hydrolysis cell culture cytotoxicity mtt reagent nadh (nicotinamide adenine dinucleotide). |
| title | STUDIES OF MAGNESIUM AND PHOSPHORUS COMBINED MEDICATION BASED ON CASEIN |
| title_full | STUDIES OF MAGNESIUM AND PHOSPHORUS COMBINED MEDICATION BASED ON CASEIN |
| title_fullStr | STUDIES OF MAGNESIUM AND PHOSPHORUS COMBINED MEDICATION BASED ON CASEIN |
| title_full_unstemmed | STUDIES OF MAGNESIUM AND PHOSPHORUS COMBINED MEDICATION BASED ON CASEIN |
| title_short | STUDIES OF MAGNESIUM AND PHOSPHORUS COMBINED MEDICATION BASED ON CASEIN |
| title_sort | studies of magnesium and phosphorus combined medication based on casein |
| topic | key words: magnesium phosphorus casein chelate in vitro hydrolysis cell culture cytotoxicity mtt reagent nadh (nicotinamide adenine dinucleotide). |
| url | http://biotechnology.kiev.ua/images/5_2021/palonko_5_2021.pdf |
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