Follow up the antibodies titer against Newcastle disease virus in broiler breeders using ELISA test
The study period carried out from 25 April 2018 till 21 May 2019 through the rearing and production period including totally of 24000 birds (20800 females, 3200 males). The number of tested blood samples was 452 divided to 255 samples at the rearing period, 143 samples at the production period and 5...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2020-04-01
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| Series: | Iraqi Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | https://vetmedmosul.com/article_164500_c938946272ee95b7ff1454abc9d8e025.pdf |
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| author | Fanar A. Isihak Salah M. Hassan Balqees Z. Shaker Yasir A. Salih |
| author_facet | Fanar A. Isihak Salah M. Hassan Balqees Z. Shaker Yasir A. Salih |
| author_sort | Fanar A. Isihak |
| collection | DOAJ |
| description | The study period carried out from 25 April 2018 till 21 May 2019 through the rearing and production period including totally of 24000 birds (20800 females, 3200 males). The number of tested blood samples was 452 divided to 255 samples at the rearing period, 143 samples at the production period and 54 samples of offspring. The results of antibodies titer in the sera of non-vaccinated broiler breeders obtained by ELISA showed the maternal derived antibodies titer for 28 samples at 0-5 week/day of age was 5716±612.7, this titer decreased gradually at 3-1 week/day age till to 1075±234) Then the titer was elevated increasingly after vaccination with both live attenuated and inactivated vaccines and reach to peak 37512±2049.4 at 20-1 week/day age. Whereas the bimodal graduation of antibodies titer showed at production period till to end of study. The mean of maternally antibodies titer in the tested sera of the offspring chicks 0-1 week/day that hatched from parent flocks at 32, 39 and 48 weeks of age was 9012±872.4, 6591±368.1 and 4831±982.7 respectively. Thus, we concluded the repetitive vaccination of broiler breeders flock with live vaccine as well as inactivated vaccine is very necessary in endemic areas and ELISA is a good serological test for following, checking and monitoring of immune status of poultry flocks periodically. |
| first_indexed | 2024-12-14T03:06:46Z |
| format | Article |
| id | doaj.art-6c98ceb9e9824807a71efa982b10cd0c |
| institution | Directory Open Access Journal |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| last_indexed | 2024-12-14T03:06:46Z |
| publishDate | 2020-04-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj.art-6c98ceb9e9824807a71efa982b10cd0c2022-12-21T23:19:23ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552020-04-0134229529910.33899/ijvs.2019.125931.1189164500Follow up the antibodies titer against Newcastle disease virus in broiler breeders using ELISA testFanar A. Isihak0Salah M. Hassan1Balqees Z. Shaker2Yasir A. Salih3Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Pathology and Poultry Diseases, College of Veterinary Medicine, Al-Qasim Green University, Babylon, IraqTechnical sector, Kosar Company for Agriculture and Poultry, Erbil, IraqTechnical sector, Kosar Company for Agriculture and Poultry, Erbil, IraqThe study period carried out from 25 April 2018 till 21 May 2019 through the rearing and production period including totally of 24000 birds (20800 females, 3200 males). The number of tested blood samples was 452 divided to 255 samples at the rearing period, 143 samples at the production period and 54 samples of offspring. The results of antibodies titer in the sera of non-vaccinated broiler breeders obtained by ELISA showed the maternal derived antibodies titer for 28 samples at 0-5 week/day of age was 5716±612.7, this titer decreased gradually at 3-1 week/day age till to 1075±234) Then the titer was elevated increasingly after vaccination with both live attenuated and inactivated vaccines and reach to peak 37512±2049.4 at 20-1 week/day age. Whereas the bimodal graduation of antibodies titer showed at production period till to end of study. The mean of maternally antibodies titer in the tested sera of the offspring chicks 0-1 week/day that hatched from parent flocks at 32, 39 and 48 weeks of age was 9012±872.4, 6591±368.1 and 4831±982.7 respectively. Thus, we concluded the repetitive vaccination of broiler breeders flock with live vaccine as well as inactivated vaccine is very necessary in endemic areas and ELISA is a good serological test for following, checking and monitoring of immune status of poultry flocks periodically.https://vetmedmosul.com/article_164500_c938946272ee95b7ff1454abc9d8e025.pdfnewcastle diseaseantibodieselisabroiler breedersvaccine |
| spellingShingle | Fanar A. Isihak Salah M. Hassan Balqees Z. Shaker Yasir A. Salih Follow up the antibodies titer against Newcastle disease virus in broiler breeders using ELISA test Iraqi Journal of Veterinary Sciences newcastle disease antibodies elisa broiler breeders vaccine |
| title | Follow up the antibodies titer against Newcastle disease virus in broiler breeders using ELISA test |
| title_full | Follow up the antibodies titer against Newcastle disease virus in broiler breeders using ELISA test |
| title_fullStr | Follow up the antibodies titer against Newcastle disease virus in broiler breeders using ELISA test |
| title_full_unstemmed | Follow up the antibodies titer against Newcastle disease virus in broiler breeders using ELISA test |
| title_short | Follow up the antibodies titer against Newcastle disease virus in broiler breeders using ELISA test |
| title_sort | follow up the antibodies titer against newcastle disease virus in broiler breeders using elisa test |
| topic | newcastle disease antibodies elisa broiler breeders vaccine |
| url | https://vetmedmosul.com/article_164500_c938946272ee95b7ff1454abc9d8e025.pdf |
| work_keys_str_mv | AT fanaraisihak followuptheantibodiestiteragainstnewcastlediseasevirusinbroilerbreedersusingelisatest AT salahmhassan followuptheantibodiestiteragainstnewcastlediseasevirusinbroilerbreedersusingelisatest AT balqeeszshaker followuptheantibodiestiteragainstnewcastlediseasevirusinbroilerbreedersusingelisatest AT yasirasalih followuptheantibodiestiteragainstnewcastlediseasevirusinbroilerbreedersusingelisatest |