Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line
Tapak dara or Madagascar Periwinkle (Catharanthus roseus [L] G.Don), a natural plant, is empirically reported to have promising anticancer activity. To elucidate its mechanism, a research was conducted to investigate the possible ethanol extract of C. roseus in inducing apoptosis on breast cancer c...
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| Format: | Article |
| Language: | English |
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Indonesian Society for Cancer Chemoprevention
2010-06-01
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| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/17 |
| _version_ | 1828336526806745088 |
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| author | Wahyu Widowati Tjandrawati Mozef Chandra Risdian Hana Ratnawati Susi Tjahyani Ferry Sandra |
| author_facet | Wahyu Widowati Tjandrawati Mozef Chandra Risdian Hana Ratnawati Susi Tjahyani Ferry Sandra |
| author_sort | Wahyu Widowati |
| collection | DOAJ |
| description | Tapak dara or Madagascar Periwinkle (Catharanthus roseus [L] G.Don), a natural plant, is empirically reported to have promising anticancer activity. To elucidate its mechanism, a research was conducted to investigate the possible ethanol extract of C. roseus in inducing apoptosis on breast cancer cell line (T47D). Antioxidant activity of C. roseus was investigated as well. Sub-G1 flowcytometric apoptotic analysis result showed that extract of C. roseus at 6.25 μg/mL induced apoptosis for 26.365%. Increasing extract concentration resulted an increasing apoptotic level as well, extract at concentration of 12.5 μg/mL induced apoptosis for 22.235%. Meanwhile doxorubicin at concentration of 10 μg/mL induced apoptosis for 36.055%. The antioxidant activity was determined by using in vitro assay: inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. Antioxidant activity of C. roseus extract were compared to quercetin and butylated hydroxyanisol (BHA), as positive controls. The results showed that DPPH IC50 of C. roseus extract, quercetin and BHA were 358.411 μg/mL, 19.200 μg/mL and 94.178 μg/mL, respectively. We suggest that C. roseus extract had a potential anticancer activity by inducing apoptosis.
Key words : antioxidant, DPPH, Catharanthus roseus, apoptosis, breast cancer, T47D |
| first_indexed | 2024-04-13T22:02:03Z |
| format | Article |
| id | doaj.art-6c99442794114fd29fbdb4929673bd10 |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-04-13T22:02:03Z |
| publishDate | 2010-06-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-6c99442794114fd29fbdb4929673bd102022-12-22T02:28:03ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892010-06-01129910710.14499/indonesianjcanchemoprev1iss2pp99-10718Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell LineWahyu Widowati0Tjandrawati Mozef1Chandra Risdian2Hana Ratnawati3Susi Tjahyani4Ferry Sandra5Medical Research Center, Faculty of Medicine, Maranatha Christian University, BandungThe Indonesian Institute of Sciences, BandungThe Indonesian Institute of Sciences, BandungMedical Research Center, Faculty of Medicine, Maranatha Christian University, BandungMedical Research Center, Faculty of Medicine, Maranatha Christian University, BandungStem Cell and Center Institute, JakartaTapak dara or Madagascar Periwinkle (Catharanthus roseus [L] G.Don), a natural plant, is empirically reported to have promising anticancer activity. To elucidate its mechanism, a research was conducted to investigate the possible ethanol extract of C. roseus in inducing apoptosis on breast cancer cell line (T47D). Antioxidant activity of C. roseus was investigated as well. Sub-G1 flowcytometric apoptotic analysis result showed that extract of C. roseus at 6.25 μg/mL induced apoptosis for 26.365%. Increasing extract concentration resulted an increasing apoptotic level as well, extract at concentration of 12.5 μg/mL induced apoptosis for 22.235%. Meanwhile doxorubicin at concentration of 10 μg/mL induced apoptosis for 36.055%. The antioxidant activity was determined by using in vitro assay: inhibition of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. Antioxidant activity of C. roseus extract were compared to quercetin and butylated hydroxyanisol (BHA), as positive controls. The results showed that DPPH IC50 of C. roseus extract, quercetin and BHA were 358.411 μg/mL, 19.200 μg/mL and 94.178 μg/mL, respectively. We suggest that C. roseus extract had a potential anticancer activity by inducing apoptosis. Key words : antioxidant, DPPH, Catharanthus roseus, apoptosis, breast cancer, T47Dhttps://ijcc.chemoprev.org/index.php/ijcc/article/view/17 |
| spellingShingle | Wahyu Widowati Tjandrawati Mozef Chandra Risdian Hana Ratnawati Susi Tjahyani Ferry Sandra Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line |
| title_full | Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line |
| title_fullStr | Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line |
| title_full_unstemmed | Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line |
| title_short | Apoptosis and Antioxidant Activities of Catharanthus rosues [L] G.Don Extract on Breast Cancer Cell Line |
| title_sort | apoptosis and antioxidant activities of catharanthus rosues l g don extract on breast cancer cell line |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/17 |
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