Regrowth of Microcosm Biofilms on Titanium Surfaces After Various Antimicrobial Treatments
Objectives: Our aim of this work was to investigate the regrowth of implant-related biofilms after various antimicrobial treatments in vitro.Methods: Saliva-derived microcosm biofilms were grown on titanium discs in an active attachment model. Treatments including hydrogen peroxide (HP), citric acid...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02693/full |
| _version_ | 1818368204561448960 |
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| author | Qi Han Qi Han Yaling Jiang Yaling Jiang Bernd W. Brandt Jingmei Yang Yu Chen Mark J. Buijs Wim Crielaard Lei Cheng Dongmei Deng |
| author_facet | Qi Han Qi Han Yaling Jiang Yaling Jiang Bernd W. Brandt Jingmei Yang Yu Chen Mark J. Buijs Wim Crielaard Lei Cheng Dongmei Deng |
| author_sort | Qi Han |
| collection | DOAJ |
| description | Objectives: Our aim of this work was to investigate the regrowth of implant-related biofilms after various antimicrobial treatments in vitro.Methods: Saliva-derived microcosm biofilms were grown on titanium discs in an active attachment model. Treatments including hydrogen peroxide (HP), citric acid (CA), chlorhexidine (CHX), and distilled water (control), at different concentrations, were applied to 2-day biofilms for 1 or 5 min. The viability, lactic acid production, and composition of the biofilms were followed for 3 days. The biofilm composition was analyzed by 16S rDNA amplicon sequencing.Results: The short treatments of CA, CHX, and HP resulted in a 2–3 log reduction in biofilm viability and lactic acid production immediately. However, both parameters returned to the pre-treatment level within 2 days due to biofilm regrowth. The alpha diversity of the regrown biofilms in antimicrobial-treated groups tended to decrease, whereas the diversity of those in water-treated group increased. The composition of the regrown biofilms altered compared to those before treatments. Streptococcus and Enterobacteriaceae were enriched in the regrown biofilms.Conclusions: Although the antimicrobial treatments were efficient, the multi-species biofilms were indeed able to regrow within 2 days. The regrown biofilms display an altered microbial diversity and composition, which in the oral cavity may lead to an aggressive infection. |
| first_indexed | 2024-12-13T23:04:14Z |
| format | Article |
| id | doaj.art-6c9a6b2ec56b4c62bbc00c27d4f3cada |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T23:04:14Z |
| publishDate | 2019-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-6c9a6b2ec56b4c62bbc00c27d4f3cada2022-12-21T23:28:19ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-11-011010.3389/fmicb.2019.02693490805Regrowth of Microcosm Biofilms on Titanium Surfaces After Various Antimicrobial TreatmentsQi Han0Qi Han1Yaling Jiang2Yaling Jiang3Bernd W. Brandt4Jingmei Yang5Yu Chen6Mark J. Buijs7Wim Crielaard8Lei Cheng9Dongmei Deng10State Key Laboratory of Oral Disease, National Clinical Research Center for Oral Diseases, Department of Pathology, Sichuan University, Chengdu, ChinaDepartment of Preventive Dentistry, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, NetherlandsDepartment of Preventive Dentistry, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, NetherlandsState Key Laboratory of Oral Disease, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodonics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Preventive Dentistry, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, NetherlandsState Key Laboratory of Oral Disease, National Clinical Research Center for Oral Diseases, Department of Periodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaState Key Laboratory of Oral Disease, National Clinical Research Center for Oral Diseases, Department of Pathology, Sichuan University, Chengdu, ChinaDepartment of Preventive Dentistry, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, NetherlandsDepartment of Preventive Dentistry, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, NetherlandsState Key Laboratory of Oral Disease, National Clinical Research Center for Oral Diseases, Department of Cariology and Endodonics, West China Hospital of Stomatology, Sichuan University, Chengdu, ChinaDepartment of Preventive Dentistry, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University Amsterdam, Amsterdam, NetherlandsObjectives: Our aim of this work was to investigate the regrowth of implant-related biofilms after various antimicrobial treatments in vitro.Methods: Saliva-derived microcosm biofilms were grown on titanium discs in an active attachment model. Treatments including hydrogen peroxide (HP), citric acid (CA), chlorhexidine (CHX), and distilled water (control), at different concentrations, were applied to 2-day biofilms for 1 or 5 min. The viability, lactic acid production, and composition of the biofilms were followed for 3 days. The biofilm composition was analyzed by 16S rDNA amplicon sequencing.Results: The short treatments of CA, CHX, and HP resulted in a 2–3 log reduction in biofilm viability and lactic acid production immediately. However, both parameters returned to the pre-treatment level within 2 days due to biofilm regrowth. The alpha diversity of the regrown biofilms in antimicrobial-treated groups tended to decrease, whereas the diversity of those in water-treated group increased. The composition of the regrown biofilms altered compared to those before treatments. Streptococcus and Enterobacteriaceae were enriched in the regrown biofilms.Conclusions: Although the antimicrobial treatments were efficient, the multi-species biofilms were indeed able to regrow within 2 days. The regrown biofilms display an altered microbial diversity and composition, which in the oral cavity may lead to an aggressive infection.https://www.frontiersin.org/article/10.3389/fmicb.2019.02693/fullperi-implantitisantimicrobialsbiofilm regrowth16S rDNAmicrocosmoral microbiome |
| spellingShingle | Qi Han Qi Han Yaling Jiang Yaling Jiang Bernd W. Brandt Jingmei Yang Yu Chen Mark J. Buijs Wim Crielaard Lei Cheng Dongmei Deng Regrowth of Microcosm Biofilms on Titanium Surfaces After Various Antimicrobial Treatments Frontiers in Microbiology peri-implantitis antimicrobials biofilm regrowth 16S rDNA microcosm oral microbiome |
| title | Regrowth of Microcosm Biofilms on Titanium Surfaces After Various Antimicrobial Treatments |
| title_full | Regrowth of Microcosm Biofilms on Titanium Surfaces After Various Antimicrobial Treatments |
| title_fullStr | Regrowth of Microcosm Biofilms on Titanium Surfaces After Various Antimicrobial Treatments |
| title_full_unstemmed | Regrowth of Microcosm Biofilms on Titanium Surfaces After Various Antimicrobial Treatments |
| title_short | Regrowth of Microcosm Biofilms on Titanium Surfaces After Various Antimicrobial Treatments |
| title_sort | regrowth of microcosm biofilms on titanium surfaces after various antimicrobial treatments |
| topic | peri-implantitis antimicrobials biofilm regrowth 16S rDNA microcosm oral microbiome |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02693/full |
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