Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (<i>Citellus dauricus</i> Brandt)
The goal of this study is to explore the relationship between altered circulating adiponectin concentration, ovarian tissue morphology, ovarian steroidogenesis, and sex hormone production in ovaries of wild ground squirrels. The ovarian mass differed significantly during the breeding and non-breedin...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-11-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/23/14698 |
| _version_ | 1797463144100003840 |
|---|---|
| author | Sijie Fan Wenjing Lu Haolin Zhang Zhengrong Yuan Yingying Han Qiang Weng |
| author_facet | Sijie Fan Wenjing Lu Haolin Zhang Zhengrong Yuan Yingying Han Qiang Weng |
| author_sort | Sijie Fan |
| collection | DOAJ |
| description | The goal of this study is to explore the relationship between altered circulating adiponectin concentration, ovarian tissue morphology, ovarian steroidogenesis, and sex hormone production in ovaries of wild ground squirrels. The ovarian mass differed significantly during the breeding and non-breeding seasons, and the circulating estradiol and progesterone concentrations were significantly higher in the breeding season, while the circulating adiponectin level was significantly lower. The expression levels of gonadotropin receptors (FSHR and LHR) and steroidogenic enzymes (StAR, P450scc, P450arom, and 3β-HSD) were significantly higher during the breeding season. Comparing the ovarian transcriptome data of wild ground squirrels between the two periods, we found that some differentially expressed genes were enriched for ovarian steroidogenesis and the adipocytokine signaling pathway, which correlated with our present results. Notably, the MAPK signaling pathway was also enriched and its related genes (<i>Erk1</i>, <i>p38 Mapk</i>, <i>Jnk</i>) were up-regulated by qPCR during the non-breeding season. These findings suggested that adiponectin may be involved in the regulation of seasonal changes in the ovarian function of wild ground squirrels, possibly by acting on the MAPK signaling pathway to regulate sex steroidogenesis in the ovaries. |
| first_indexed | 2024-03-09T17:46:30Z |
| format | Article |
| id | doaj.art-6c9bdf0c31124be8a09bb10acc9e5b2f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:46:30Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6c9bdf0c31124be8a09bb10acc9e5b2f2023-11-24T11:06:22ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123231469810.3390/ijms232314698Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (<i>Citellus dauricus</i> Brandt)Sijie Fan0Wenjing Lu1Haolin Zhang2Zhengrong Yuan3Yingying Han4Qiang Weng5College of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaCollege of Biological Science and Technology, Beijing Forestry University, Beijing 100083, ChinaThe goal of this study is to explore the relationship between altered circulating adiponectin concentration, ovarian tissue morphology, ovarian steroidogenesis, and sex hormone production in ovaries of wild ground squirrels. The ovarian mass differed significantly during the breeding and non-breeding seasons, and the circulating estradiol and progesterone concentrations were significantly higher in the breeding season, while the circulating adiponectin level was significantly lower. The expression levels of gonadotropin receptors (FSHR and LHR) and steroidogenic enzymes (StAR, P450scc, P450arom, and 3β-HSD) were significantly higher during the breeding season. Comparing the ovarian transcriptome data of wild ground squirrels between the two periods, we found that some differentially expressed genes were enriched for ovarian steroidogenesis and the adipocytokine signaling pathway, which correlated with our present results. Notably, the MAPK signaling pathway was also enriched and its related genes (<i>Erk1</i>, <i>p38 Mapk</i>, <i>Jnk</i>) were up-regulated by qPCR during the non-breeding season. These findings suggested that adiponectin may be involved in the regulation of seasonal changes in the ovarian function of wild ground squirrels, possibly by acting on the MAPK signaling pathway to regulate sex steroidogenesis in the ovaries.https://www.mdpi.com/1422-0067/23/23/14698adiponectinadiponectin receptorMAPK signaling pathwayovarysteroidogenesiswild ground squirrels |
| spellingShingle | Sijie Fan Wenjing Lu Haolin Zhang Zhengrong Yuan Yingying Han Qiang Weng Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (<i>Citellus dauricus</i> Brandt) International Journal of Molecular Sciences adiponectin adiponectin receptor MAPK signaling pathway ovary steroidogenesis wild ground squirrels |
| title | Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (<i>Citellus dauricus</i> Brandt) |
| title_full | Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (<i>Citellus dauricus</i> Brandt) |
| title_fullStr | Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (<i>Citellus dauricus</i> Brandt) |
| title_full_unstemmed | Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (<i>Citellus dauricus</i> Brandt) |
| title_short | Seasonal Change in Adiponectin Associated with Ovarian Morphology and Function in Wild Ground Squirrels (<i>Citellus dauricus</i> Brandt) |
| title_sort | seasonal change in adiponectin associated with ovarian morphology and function in wild ground squirrels i citellus dauricus i brandt |
| topic | adiponectin adiponectin receptor MAPK signaling pathway ovary steroidogenesis wild ground squirrels |
| url | https://www.mdpi.com/1422-0067/23/23/14698 |
| work_keys_str_mv | AT sijiefan seasonalchangeinadiponectinassociatedwithovarianmorphologyandfunctioninwildgroundsquirrelsicitellusdauricusibrandt AT wenjinglu seasonalchangeinadiponectinassociatedwithovarianmorphologyandfunctioninwildgroundsquirrelsicitellusdauricusibrandt AT haolinzhang seasonalchangeinadiponectinassociatedwithovarianmorphologyandfunctioninwildgroundsquirrelsicitellusdauricusibrandt AT zhengrongyuan seasonalchangeinadiponectinassociatedwithovarianmorphologyandfunctioninwildgroundsquirrelsicitellusdauricusibrandt AT yingyinghan seasonalchangeinadiponectinassociatedwithovarianmorphologyandfunctioninwildgroundsquirrelsicitellusdauricusibrandt AT qiangweng seasonalchangeinadiponectinassociatedwithovarianmorphologyandfunctioninwildgroundsquirrelsicitellusdauricusibrandt |