Relative expression profile of Kisspeptin (Kiss1-Kiss1r) and gonadotrophin receptor in the ovarian follicular tissue and their association in the Buffalo (Bubalus bubalis)
In the past decade, kisspeptin research was primarily focussed on the regulation of GnRH release in hypothalamus. Present study was designed to explore the expression of extra-hypothalamic kisspeptinergic (Kiss1- Kiss1r) system in the follicular compartment of buffalo ovary. Buffalo genitalia (n=32...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2022-02-01
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| Series: | Indian Journal of Animal Sciences |
| Subjects: | |
| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/106785 |
| _version_ | 1811169058324742144 |
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| author | Girish Kumar Mishra Manas Patra Kumar Laishram Kipjen Singh Vikramaditya Upmanyu Soumendu Chakravarti Mathesh Karikalan Manjit Panigrahi Sanjay Kumar Singh Goutam Kumar Das Krishnaswamy Narayanan Harendra Kumar |
| author_facet | Girish Kumar Mishra Manas Patra Kumar Laishram Kipjen Singh Vikramaditya Upmanyu Soumendu Chakravarti Mathesh Karikalan Manjit Panigrahi Sanjay Kumar Singh Goutam Kumar Das Krishnaswamy Narayanan Harendra Kumar |
| author_sort | Girish Kumar Mishra |
| collection | DOAJ |
| description |
In the past decade, kisspeptin research was primarily focussed on the regulation of GnRH release in hypothalamus. Present study was designed to explore the expression of extra-hypothalamic kisspeptinergic (Kiss1- Kiss1r) system in the follicular compartment of buffalo ovary. Buffalo genitalia (n=32) were collected immediately after exsanguinations and categorized into early luteal (EL), mid luteal (ML), follicular (FL) and acyclic (n=8 per group), based on the gross ovarian morphology. Ovarian follicular tissue samples were subjected to total RNA extraction, cDNA synthesis and qPCR amplification of Kiss1, Kiss1r, follicle stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) along with an endogenous control (β-actin) gene. The expression of ovarian Kiss1 transcripts was abundant in the cyclic than acyclic stage. The fold change was significantly upregulated in ML (66.79 fold) followed by EL (28.64 fold) and FL (14.09 fold) stages against the acyclic stage (calibrator). Similarly, the Kiss1r expression was highest at ML (77.26 fold). The expression of FSHR was upregulated at FL (15.08 fold) stage in response to follicular activity and subsequently observed to be down regulated at EL (0.09 times) and ML (0.27 times). Further, the expression of Kiss1 was positively correlated with FSHR only at ML and FL. From this study, it could be concluded that Kiss1 and Kiss1r are expressed in the buffalo ovarian follicle and their expression is associated with the stage of estrous cycle.
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| first_indexed | 2024-04-10T16:35:39Z |
| format | Article |
| id | doaj.art-6c9cd770f6b446f4b178ac59c322da19 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T16:35:39Z |
| publishDate | 2022-02-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-6c9cd770f6b446f4b178ac59c322da192023-02-08T11:30:12ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272022-02-0192510.56093/ijans.v92i5.106785Relative expression profile of Kisspeptin (Kiss1-Kiss1r) and gonadotrophin receptor in the ovarian follicular tissue and their association in the Buffalo (Bubalus bubalis)Girish Kumar Mishra0Manas Patra Kumar1Laishram Kipjen Singh2Vikramaditya Upmanyu3Soumendu Chakravarti4Mathesh Karikalan5Manjit Panigrahi6Sanjay Kumar Singh7Goutam Kumar Das8Krishnaswamy Narayanan9Harendra Kumar10Dau Shri Vasudev Chandrakar Kamdhenu Vishwavidyalaya, Durg, ChhattisgarhScientist, ICAR- Indian Veterinary Research Institute, Izatnagar, U.P., 243 122ICAR-NDRI, KarnalICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122ICAR-Indian Veterinary Research Institute, Izatnagar, U.P., 243 122 In the past decade, kisspeptin research was primarily focussed on the regulation of GnRH release in hypothalamus. Present study was designed to explore the expression of extra-hypothalamic kisspeptinergic (Kiss1- Kiss1r) system in the follicular compartment of buffalo ovary. Buffalo genitalia (n=32) were collected immediately after exsanguinations and categorized into early luteal (EL), mid luteal (ML), follicular (FL) and acyclic (n=8 per group), based on the gross ovarian morphology. Ovarian follicular tissue samples were subjected to total RNA extraction, cDNA synthesis and qPCR amplification of Kiss1, Kiss1r, follicle stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHR) along with an endogenous control (β-actin) gene. The expression of ovarian Kiss1 transcripts was abundant in the cyclic than acyclic stage. The fold change was significantly upregulated in ML (66.79 fold) followed by EL (28.64 fold) and FL (14.09 fold) stages against the acyclic stage (calibrator). Similarly, the Kiss1r expression was highest at ML (77.26 fold). The expression of FSHR was upregulated at FL (15.08 fold) stage in response to follicular activity and subsequently observed to be down regulated at EL (0.09 times) and ML (0.27 times). Further, the expression of Kiss1 was positively correlated with FSHR only at ML and FL. From this study, it could be concluded that Kiss1 and Kiss1r are expressed in the buffalo ovarian follicle and their expression is associated with the stage of estrous cycle. https://epubs.icar.org.in/index.php/IJAnS/article/view/106785BuffaloOvarian tissueKisspeptin and its receptor |
| spellingShingle | Girish Kumar Mishra Manas Patra Kumar Laishram Kipjen Singh Vikramaditya Upmanyu Soumendu Chakravarti Mathesh Karikalan Manjit Panigrahi Sanjay Kumar Singh Goutam Kumar Das Krishnaswamy Narayanan Harendra Kumar Relative expression profile of Kisspeptin (Kiss1-Kiss1r) and gonadotrophin receptor in the ovarian follicular tissue and their association in the Buffalo (Bubalus bubalis) Indian Journal of Animal Sciences Buffalo Ovarian tissue Kisspeptin and its receptor |
| title | Relative expression profile of Kisspeptin (Kiss1-Kiss1r) and gonadotrophin receptor in the ovarian follicular tissue and their association in the Buffalo (Bubalus bubalis) |
| title_full | Relative expression profile of Kisspeptin (Kiss1-Kiss1r) and gonadotrophin receptor in the ovarian follicular tissue and their association in the Buffalo (Bubalus bubalis) |
| title_fullStr | Relative expression profile of Kisspeptin (Kiss1-Kiss1r) and gonadotrophin receptor in the ovarian follicular tissue and their association in the Buffalo (Bubalus bubalis) |
| title_full_unstemmed | Relative expression profile of Kisspeptin (Kiss1-Kiss1r) and gonadotrophin receptor in the ovarian follicular tissue and their association in the Buffalo (Bubalus bubalis) |
| title_short | Relative expression profile of Kisspeptin (Kiss1-Kiss1r) and gonadotrophin receptor in the ovarian follicular tissue and their association in the Buffalo (Bubalus bubalis) |
| title_sort | relative expression profile of kisspeptin kiss1 kiss1r and gonadotrophin receptor in the ovarian follicular tissue and their association in the buffalo bubalus bubalis |
| topic | Buffalo Ovarian tissue Kisspeptin and its receptor |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/106785 |
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