Physiological and Gene Expression Responses of Six Annual Ryegrass Cultivars to Cobalt, Lead, and Nickel Stresses
Heavy metals negatively affect soil quality and crop growth. In this study, we compared the tolerance of six ryegrass cultivars to cobalt (Co<sup>2+</sup>), lead (Pb<sup>2+</sup>), and nickel (Ni<sup>2+</sup>) stresses by analyzing their physiological indexes and...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-12-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/24/13583 |
| _version_ | 1797503739709358080 |
|---|---|
| author | Siyu Qiao Ye Tao Qinghua Shan Jingang Wang Tuanyao Chai Shufang Gong Kun Qiao |
| author_facet | Siyu Qiao Ye Tao Qinghua Shan Jingang Wang Tuanyao Chai Shufang Gong Kun Qiao |
| author_sort | Siyu Qiao |
| collection | DOAJ |
| description | Heavy metals negatively affect soil quality and crop growth. In this study, we compared the tolerance of six ryegrass cultivars to cobalt (Co<sup>2+</sup>), lead (Pb<sup>2+</sup>), and nickel (Ni<sup>2+</sup>) stresses by analyzing their physiological indexes and transcript levels of genes encoding metal transporters. Compared with the other cultivars, the cultivar Lm1 showed higher germination rates and better growth under Co<sup>2+</sup>, Pb<sup>2+</sup>, or Ni<sup>2+</sup> treatments. After 48 h of Co<sup>2+</sup> treatment, the total antioxidant capacity of all six ryegrass cultivars was significantly increased, especially that of Lm1. In contrast, under Pb<sup>2+</sup> stress, total antioxidant capacity of five cultivars was significantly decreased, but that of Lm1 was unaffected at 24 h. Staining with Evans blue dye showed that the roots of Lm1 were less injured than were roots of the other five ryegrass cultivars by Co<sup>2+</sup>, Pb<sup>2+</sup>, and Ni<sup>2+</sup>. Lm1 translocated and accumulated lesser Co<sup>2+</sup>, Pb<sup>2+</sup>, and Ni<sup>2+</sup> than other cultivars. In Lm1, genes encoding heavy metal transporters were differentially expressed between the shoots and roots in response to Co<sup>2+</sup>, Pb<sup>2+</sup>, and Ni<sup>2+</sup>. The aim of these researches could help find potential resource for phytoremediation of heavy metal contamination soil. The identified genes related to resistance will be useful targets for molecular breeding. |
| first_indexed | 2024-03-10T03:54:38Z |
| format | Article |
| id | doaj.art-6c9ebc168ac6485792d33f79480341fe |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:54:38Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6c9ebc168ac6485792d33f79480341fe2023-11-23T08:48:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0122241358310.3390/ijms222413583Physiological and Gene Expression Responses of Six Annual Ryegrass Cultivars to Cobalt, Lead, and Nickel StressesSiyu Qiao0Ye Tao1Qinghua Shan2Jingang Wang3Tuanyao Chai4Shufang Gong5Kun Qiao6College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Life Science, University of Chinese Academy of Sciences, Beijing, 100049, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaCollege of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, ChinaHeavy metals negatively affect soil quality and crop growth. In this study, we compared the tolerance of six ryegrass cultivars to cobalt (Co<sup>2+</sup>), lead (Pb<sup>2+</sup>), and nickel (Ni<sup>2+</sup>) stresses by analyzing their physiological indexes and transcript levels of genes encoding metal transporters. Compared with the other cultivars, the cultivar Lm1 showed higher germination rates and better growth under Co<sup>2+</sup>, Pb<sup>2+</sup>, or Ni<sup>2+</sup> treatments. After 48 h of Co<sup>2+</sup> treatment, the total antioxidant capacity of all six ryegrass cultivars was significantly increased, especially that of Lm1. In contrast, under Pb<sup>2+</sup> stress, total antioxidant capacity of five cultivars was significantly decreased, but that of Lm1 was unaffected at 24 h. Staining with Evans blue dye showed that the roots of Lm1 were less injured than were roots of the other five ryegrass cultivars by Co<sup>2+</sup>, Pb<sup>2+</sup>, and Ni<sup>2+</sup>. Lm1 translocated and accumulated lesser Co<sup>2+</sup>, Pb<sup>2+</sup>, and Ni<sup>2+</sup> than other cultivars. In Lm1, genes encoding heavy metal transporters were differentially expressed between the shoots and roots in response to Co<sup>2+</sup>, Pb<sup>2+</sup>, and Ni<sup>2+</sup>. The aim of these researches could help find potential resource for phytoremediation of heavy metal contamination soil. The identified genes related to resistance will be useful targets for molecular breeding.https://www.mdpi.com/1422-0067/22/24/13583ryegrassCo/Pb/Ni tolerancephysiological indexesgene expression |
| spellingShingle | Siyu Qiao Ye Tao Qinghua Shan Jingang Wang Tuanyao Chai Shufang Gong Kun Qiao Physiological and Gene Expression Responses of Six Annual Ryegrass Cultivars to Cobalt, Lead, and Nickel Stresses International Journal of Molecular Sciences ryegrass Co/Pb/Ni tolerance physiological indexes gene expression |
| title | Physiological and Gene Expression Responses of Six Annual Ryegrass Cultivars to Cobalt, Lead, and Nickel Stresses |
| title_full | Physiological and Gene Expression Responses of Six Annual Ryegrass Cultivars to Cobalt, Lead, and Nickel Stresses |
| title_fullStr | Physiological and Gene Expression Responses of Six Annual Ryegrass Cultivars to Cobalt, Lead, and Nickel Stresses |
| title_full_unstemmed | Physiological and Gene Expression Responses of Six Annual Ryegrass Cultivars to Cobalt, Lead, and Nickel Stresses |
| title_short | Physiological and Gene Expression Responses of Six Annual Ryegrass Cultivars to Cobalt, Lead, and Nickel Stresses |
| title_sort | physiological and gene expression responses of six annual ryegrass cultivars to cobalt lead and nickel stresses |
| topic | ryegrass Co/Pb/Ni tolerance physiological indexes gene expression |
| url | https://www.mdpi.com/1422-0067/22/24/13583 |
| work_keys_str_mv | AT siyuqiao physiologicalandgeneexpressionresponsesofsixannualryegrasscultivarstocobaltleadandnickelstresses AT yetao physiologicalandgeneexpressionresponsesofsixannualryegrasscultivarstocobaltleadandnickelstresses AT qinghuashan physiologicalandgeneexpressionresponsesofsixannualryegrasscultivarstocobaltleadandnickelstresses AT jingangwang physiologicalandgeneexpressionresponsesofsixannualryegrasscultivarstocobaltleadandnickelstresses AT tuanyaochai physiologicalandgeneexpressionresponsesofsixannualryegrasscultivarstocobaltleadandnickelstresses AT shufanggong physiologicalandgeneexpressionresponsesofsixannualryegrasscultivarstocobaltleadandnickelstresses AT kunqiao physiologicalandgeneexpressionresponsesofsixannualryegrasscultivarstocobaltleadandnickelstresses |