IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORES

Techniques of establishing Angiopleris evecta plants  in vitro were studied. Soaking of A. evecta spores in water for 24 hours markedly reduced spore contamination. Soaking of the spores in 1 -2 % of sodium hypochlorite for  less  than 5 minutes allowed satisfactory disinfestation without affec...

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Main Authors: POONAM BHATIA, DAMIEN CUPITT, NANJAPPA ASHWATH
Format: Article
Language:English
Published: SEAMEO, BIOTROP 2011-11-01
Series:Biotropia: The Southeast Asian Journal of Tropical Biology
Online Access:https://journal.biotrop.org/index.php/biotropia/article/view/161
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author POONAM BHATIA
DAMIEN CUPITT
NANJAPPA ASHWATH
author_facet POONAM BHATIA
DAMIEN CUPITT
NANJAPPA ASHWATH
author_sort POONAM BHATIA
collection DOAJ
description Techniques of establishing Angiopleris evecta plants  in vitro were studied. Soaking of A. evecta spores in water for 24 hours markedly reduced spore contamination. Soaking of the spores in 1 -2 % of sodium hypochlorite for  less  than 5 minutes allowed satisfactory disinfestation without affecting spore viability. Lower concentration of minerals (1/4 MS), presence of charcoal in the medium and exposure of the spores to light were crucial for spore germination and gainetophytc development of A. evecta. Keywords:   Angiopleris evecta I King fern / spore / MS medium / bleach / light / tissue culture / sporophyte / gamctophytc
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spelling doaj.art-6ca090ee4fb240febeae84bcd553d4302023-08-02T08:16:26ZengSEAMEO, BIOTROPBiotropia: The Southeast Asian Journal of Tropical Biology0215-63341907-770X2011-11-011610.11598/btb.2001.0.16.161IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORESPOONAM BHATIA0DAMIEN CUPITT1NANJAPPA ASHWATH2School of Biological and Environmental Sciences,Primary Industries Research Centre, School of Biological and Environmental Sciences, Central Queensland University, Rockhampton, QLD 4702, AustraliaPrimary Industries Research Centre, School of Biological and Environmental Sciences, Central Queensland University, Rockhampton, QLD 4702, AustraliaTechniques of establishing Angiopleris evecta plants  in vitro were studied. Soaking of A. evecta spores in water for 24 hours markedly reduced spore contamination. Soaking of the spores in 1 -2 % of sodium hypochlorite for  less  than 5 minutes allowed satisfactory disinfestation without affecting spore viability. Lower concentration of minerals (1/4 MS), presence of charcoal in the medium and exposure of the spores to light were crucial for spore germination and gainetophytc development of A. evecta. Keywords:   Angiopleris evecta I King fern / spore / MS medium / bleach / light / tissue culture / sporophyte / gamctophytchttps://journal.biotrop.org/index.php/biotropia/article/view/161
spellingShingle POONAM BHATIA
DAMIEN CUPITT
NANJAPPA ASHWATH
IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORES
Biotropia: The Southeast Asian Journal of Tropical Biology
title IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORES
title_full IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORES
title_fullStr IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORES
title_full_unstemmed IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORES
title_short IN VITRO PROPAGATION OF ANGIOPTERIS EVECTA USING SPORES
title_sort in vitro propagation of angiopteris evecta using spores
url https://journal.biotrop.org/index.php/biotropia/article/view/161
work_keys_str_mv AT poonambhatia invitropropagationofangiopterisevectausingspores
AT damiencupitt invitropropagationofangiopterisevectausingspores
AT nanjappaashwath invitropropagationofangiopterisevectausingspores