Association of Circulating miR-145-5p and miR-let7c and Atherosclerotic Plaques in Hypertensive Patients
Aim: Hypertension is a strong risk factor for atherosclerosis. Increased carotid intima-media thickness (cIMT) and carotid plaques are considered subclinical markers of atherosclerosis. This study aimed at evaluating the serum expression of miRNAs previously related to adverse vascular remodeling an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/11/12/1840 |
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| author | Eduarda O. Z. Minin Layde R. Paim Elisangela C. P. Lopes Larissa C. M. Bueno Luís F. R. S. Carvalho-Romano Edmilson R. Marques Camila F. L. Vegian José A. Pio-Magalhães Otavio R. Coelho-Filho Andrei C. Sposito José R. Matos-Souza Wilson Nadruz Roberto Schreiber |
| author_facet | Eduarda O. Z. Minin Layde R. Paim Elisangela C. P. Lopes Larissa C. M. Bueno Luís F. R. S. Carvalho-Romano Edmilson R. Marques Camila F. L. Vegian José A. Pio-Magalhães Otavio R. Coelho-Filho Andrei C. Sposito José R. Matos-Souza Wilson Nadruz Roberto Schreiber |
| author_sort | Eduarda O. Z. Minin |
| collection | DOAJ |
| description | Aim: Hypertension is a strong risk factor for atherosclerosis. Increased carotid intima-media thickness (cIMT) and carotid plaques are considered subclinical markers of atherosclerosis. This study aimed at evaluating the serum expression of miRNAs previously related to adverse vascular remodeling and correlating them with carotid plaques and cIMT in hypertensive patients. Methods: We cross-sectionally evaluated the clinical and carotid characteristics as well as serum expression of miR-145-5p, miR-let7c, miR-92a, miR-30a and miR-451 in 177 hypertensive patients. Carotid plaques and cIMT were evaluated by ultrasound, and the expression of selected miRNAs was evaluated by a quantitative polymerase chain reaction. Results: Among all participants (age = 60.6 ± 10.7 years, 43% males), there were 59% with carotid plaques. We observed an increased expression of miR-145-5p (Fold Change = 2.0, <i>p</i> = 0.035) and miR-let7c (Fold Change = 3.8, <i>p</i> = 0.045) in participants with atherosclerotic plaque when compared to those without plaque. In the logistic regression analysis adjusted for relevant covariates, these miRNAs showed a stronger association with carotid plaques (miR-145-5p: <i>Beta ± SE</i> = 0.050 ± 0.020, <i>p</i> = 0.016 and miR-let7c: <i>Beta ± SE</i> = 0.056 ± 0.019, <i>p</i> = 0.003). Conclusions: Hypertensive patients with carotid plaques have an increased expression of miR-145-5p and miR-let7c, suggesting a potential role of these miRNAs as a biomarker for subclinical atherosclerosis in hypertensive individuals. |
| first_indexed | 2024-03-10T04:32:47Z |
| format | Article |
| id | doaj.art-6ca11753734949b8af4dec0bea254691 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T04:32:47Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-6ca11753734949b8af4dec0bea2546912023-11-23T03:59:59ZengMDPI AGBiomolecules2218-273X2021-12-011112184010.3390/biom11121840Association of Circulating miR-145-5p and miR-let7c and Atherosclerotic Plaques in Hypertensive PatientsEduarda O. Z. Minin0Layde R. Paim1Elisangela C. P. Lopes2Larissa C. M. Bueno3Luís F. R. S. Carvalho-Romano4Edmilson R. Marques5Camila F. L. Vegian6José A. Pio-Magalhães7Otavio R. Coelho-Filho8Andrei C. Sposito9José R. Matos-Souza10Wilson Nadruz11Roberto Schreiber12Department of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilDepartment of Internal Medicine, School of Medical Sciences, State University of Campinas, Campinas 13083-970, São Paulo, BrazilAim: Hypertension is a strong risk factor for atherosclerosis. Increased carotid intima-media thickness (cIMT) and carotid plaques are considered subclinical markers of atherosclerosis. This study aimed at evaluating the serum expression of miRNAs previously related to adverse vascular remodeling and correlating them with carotid plaques and cIMT in hypertensive patients. Methods: We cross-sectionally evaluated the clinical and carotid characteristics as well as serum expression of miR-145-5p, miR-let7c, miR-92a, miR-30a and miR-451 in 177 hypertensive patients. Carotid plaques and cIMT were evaluated by ultrasound, and the expression of selected miRNAs was evaluated by a quantitative polymerase chain reaction. Results: Among all participants (age = 60.6 ± 10.7 years, 43% males), there were 59% with carotid plaques. We observed an increased expression of miR-145-5p (Fold Change = 2.0, <i>p</i> = 0.035) and miR-let7c (Fold Change = 3.8, <i>p</i> = 0.045) in participants with atherosclerotic plaque when compared to those without plaque. In the logistic regression analysis adjusted for relevant covariates, these miRNAs showed a stronger association with carotid plaques (miR-145-5p: <i>Beta ± SE</i> = 0.050 ± 0.020, <i>p</i> = 0.016 and miR-let7c: <i>Beta ± SE</i> = 0.056 ± 0.019, <i>p</i> = 0.003). Conclusions: Hypertensive patients with carotid plaques have an increased expression of miR-145-5p and miR-let7c, suggesting a potential role of these miRNAs as a biomarker for subclinical atherosclerosis in hypertensive individuals.https://www.mdpi.com/2218-273X/11/12/1840hypertensioncarotid atherosclerosismiRNAs |
| spellingShingle | Eduarda O. Z. Minin Layde R. Paim Elisangela C. P. Lopes Larissa C. M. Bueno Luís F. R. S. Carvalho-Romano Edmilson R. Marques Camila F. L. Vegian José A. Pio-Magalhães Otavio R. Coelho-Filho Andrei C. Sposito José R. Matos-Souza Wilson Nadruz Roberto Schreiber Association of Circulating miR-145-5p and miR-let7c and Atherosclerotic Plaques in Hypertensive Patients Biomolecules hypertension carotid atherosclerosis miRNAs |
| title | Association of Circulating miR-145-5p and miR-let7c and Atherosclerotic Plaques in Hypertensive Patients |
| title_full | Association of Circulating miR-145-5p and miR-let7c and Atherosclerotic Plaques in Hypertensive Patients |
| title_fullStr | Association of Circulating miR-145-5p and miR-let7c and Atherosclerotic Plaques in Hypertensive Patients |
| title_full_unstemmed | Association of Circulating miR-145-5p and miR-let7c and Atherosclerotic Plaques in Hypertensive Patients |
| title_short | Association of Circulating miR-145-5p and miR-let7c and Atherosclerotic Plaques in Hypertensive Patients |
| title_sort | association of circulating mir 145 5p and mir let7c and atherosclerotic plaques in hypertensive patients |
| topic | hypertension carotid atherosclerosis miRNAs |
| url | https://www.mdpi.com/2218-273X/11/12/1840 |
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