Immune and oxidative response to linseed in the diet of periparturient Holstein cows
The aim of this research was to determine the influence of dietary replacement of n-6 with n-3 polyunsaturated fatty acids on cellular immunity and oxidative stress in the transition period dairy cows. The experiment was conducted on 20 dairy Holstein cows from 3±1 weeks before parturition until the...
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| Format: | Article |
| Language: | English |
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Elsevier
2015-01-01
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| Series: | Animal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731115000439 |
| _version_ | 1818597451339137024 |
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| author | M. Đidara N. Poljičak-Milas S. Milinković-Tur T. Mašek J. Šuran M. Pavić M. Kardum M. Šperanda |
| author_facet | M. Đidara N. Poljičak-Milas S. Milinković-Tur T. Mašek J. Šuran M. Pavić M. Kardum M. Šperanda |
| author_sort | M. Đidara |
| collection | DOAJ |
| description | The aim of this research was to determine the influence of dietary replacement of n-6 with n-3 polyunsaturated fatty acids on cellular immunity and oxidative stress in the transition period dairy cows. The experiment was conducted on 20 dairy Holstein cows from 3±1 weeks before parturition until the 6th week of lactation. Both groups were fed an iso-energetic and iso-nitrogenous diet. Soybean meal from control (C) group was replaced with linseed in the experimental (LS) group. Cellular immunity and oxidative stress were measured on days −10, 1, 21 and 42 relative to parturition. During the entire experimental period, the proportion of CD45+ cells was lower (P<0.05) in LS group compared with the C group. The phagocytosis ability and phagocytosis index of cows fed with n-3 fatty acids were significantly reduced (P<0.05) compared with the group of cows fed with n-6 fatty acids. The most severe decrease in phagocytosis ability was on day −10 and 1 relative to parturition. The activity of superoxide dismutase (P<0.05) and plasma glutathione peroxidase (P<0.05) increased around calving, although activities were not influenced by dietary treatment. Increased malondialdehyde concentration (P<0.05) was influenced by dietary n-3 fatty acids and the time relative to parturition. The immune suppression was most pronounced during periparturient period. In that matter we can conclude that not only dietary n-3 fatty acids but also oxidative stress, which reached peak at time of parturition, contributed to the reduced cellular immunity during the periparturient period. |
| first_indexed | 2024-12-16T11:48:01Z |
| format | Article |
| id | doaj.art-6ca150050b0149daa88635fc80d7029f |
| institution | Directory Open Access Journal |
| issn | 1751-7311 |
| language | English |
| last_indexed | 2024-12-16T11:48:01Z |
| publishDate | 2015-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Animal |
| spelling | doaj.art-6ca150050b0149daa88635fc80d7029f2022-12-21T22:32:47ZengElsevierAnimal1751-73112015-01-019813491354Immune and oxidative response to linseed in the diet of periparturient Holstein cowsM. Đidara0N. Poljičak-Milas1S. Milinković-Tur2T. Mašek3J. Šuran4M. Pavić5M. Kardum6M. Šperanda7Department of Animal Science, Faculty of Agriculture, K. P. Svacica 1D, 31000 Osijek, CroatiaDepartment of Pathophysiology, Veterinary Faculty, Heinzelova 55, 10000 Zagreb, CroatiaDepartment of Physiology and Radiobiology, Veterinary Faculty, Heinzelova 55, 10000 Zagreb, CroatiaDepartment of Animal Nutrition and Dietetics, Veterinary Faculty, Heinzelova 55, 10000 Zagreb, CroatiaDepartment of Pharmacology and Toxicology, Veterinary Faculty, Heinzelova 55, 10000 Zagreb, CroatiaDepartment of Animal Science, Faculty of Agriculture, K. P. Svacica 1D, 31000 Osijek, CroatiaDepartment of Pathophysiology, Veterinary Faculty, Heinzelova 55, 10000 Zagreb, CroatiaDepartment of Animal Science, Faculty of Agriculture, K. P. Svacica 1D, 31000 Osijek, CroatiaThe aim of this research was to determine the influence of dietary replacement of n-6 with n-3 polyunsaturated fatty acids on cellular immunity and oxidative stress in the transition period dairy cows. The experiment was conducted on 20 dairy Holstein cows from 3±1 weeks before parturition until the 6th week of lactation. Both groups were fed an iso-energetic and iso-nitrogenous diet. Soybean meal from control (C) group was replaced with linseed in the experimental (LS) group. Cellular immunity and oxidative stress were measured on days −10, 1, 21 and 42 relative to parturition. During the entire experimental period, the proportion of CD45+ cells was lower (P<0.05) in LS group compared with the C group. The phagocytosis ability and phagocytosis index of cows fed with n-3 fatty acids were significantly reduced (P<0.05) compared with the group of cows fed with n-6 fatty acids. The most severe decrease in phagocytosis ability was on day −10 and 1 relative to parturition. The activity of superoxide dismutase (P<0.05) and plasma glutathione peroxidase (P<0.05) increased around calving, although activities were not influenced by dietary treatment. Increased malondialdehyde concentration (P<0.05) was influenced by dietary n-3 fatty acids and the time relative to parturition. The immune suppression was most pronounced during periparturient period. In that matter we can conclude that not only dietary n-3 fatty acids but also oxidative stress, which reached peak at time of parturition, contributed to the reduced cellular immunity during the periparturient period.http://www.sciencedirect.com/science/article/pii/S1751731115000439dairy cowsimmune responseoxidative stresslinseedfatty acids |
| spellingShingle | M. Đidara N. Poljičak-Milas S. Milinković-Tur T. Mašek J. Šuran M. Pavić M. Kardum M. Šperanda Immune and oxidative response to linseed in the diet of periparturient Holstein cows Animal dairy cows immune response oxidative stress linseed fatty acids |
| title | Immune and oxidative response to linseed in the diet of periparturient Holstein cows |
| title_full | Immune and oxidative response to linseed in the diet of periparturient Holstein cows |
| title_fullStr | Immune and oxidative response to linseed in the diet of periparturient Holstein cows |
| title_full_unstemmed | Immune and oxidative response to linseed in the diet of periparturient Holstein cows |
| title_short | Immune and oxidative response to linseed in the diet of periparturient Holstein cows |
| title_sort | immune and oxidative response to linseed in the diet of periparturient holstein cows |
| topic | dairy cows immune response oxidative stress linseed fatty acids |
| url | http://www.sciencedirect.com/science/article/pii/S1751731115000439 |
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