Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides
Mogrosides are a group of health-promoting natural products that extracted from <i>Siraitia grosvenorii</i> fruit (Luo-han-guo or monk fruit), which exhibited a promising practical application in natural sweeteners and pharmaceutical development. However, the production of mogrosides is...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/18/10422 |
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| author | Jingjing Liao Tingyao Liu Lei Xie Changming Mo Xiyang Huang Shengrong Cui Xunli Jia Fusheng Lan Zuliang Luo Xiaojun Ma |
| author_facet | Jingjing Liao Tingyao Liu Lei Xie Changming Mo Xiyang Huang Shengrong Cui Xunli Jia Fusheng Lan Zuliang Luo Xiaojun Ma |
| author_sort | Jingjing Liao |
| collection | DOAJ |
| description | Mogrosides are a group of health-promoting natural products that extracted from <i>Siraitia grosvenorii</i> fruit (Luo-han-guo or monk fruit), which exhibited a promising practical application in natural sweeteners and pharmaceutical development. However, the production of mogrosides is inadequate to meet the need worldwide, and uneconomical synthetic chemistry methods are not generally recommended for structural complexity. To address this issue, an in-fusion based gene stacking strategy (IGS) for multigene stacking has been developed to assemble 6 mogrosides synthase genes in pCAMBIA1300. Metabolic engineering of <i>Nicotiana benthamiana</i> and <i>Arabidopsis thaliana</i> to produce mogrosides from 2,3-oxidosqualene was carried out. Moreover, a validated HPLC-MS/MS method was used for the quantitative analysis of mogrosides in transgenic plants. Herein, engineered <i>Arabidopsis thaliana</i> produced siamenoside I ranging from 29.65 to 1036.96 ng/g FW, and the content of mogroside III at 202.75 ng/g FW, respectively. The production of mogroside III was from 148.30 to 252.73 ng/g FW, and mogroside II-E with concentration between 339.27 and 5663.55 ng/g FW in the engineered tobacco, respectively. This study provides information potentially applicable to develop a powerful and green toolkit for the production of mogrosides. |
| first_indexed | 2024-03-09T23:46:46Z |
| format | Article |
| id | doaj.art-6ca18b95056144cfa2915ac4ea450548 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T23:46:46Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6ca18b95056144cfa2915ac4ea4505482023-11-23T16:41:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181042210.3390/ijms231810422Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse MogrosidesJingjing Liao0Tingyao Liu1Lei Xie2Changming Mo3Xiyang Huang4Shengrong Cui5Xunli Jia6Fusheng Lan7Zuliang Luo8Xiaojun Ma9The Artemisinin Research Center, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, ChinaGuangxi Crop Genetic Improvement and Biotechnology Lab, Guangxi Academy of Agricultural Sciences, Nanning 530007, ChinaGuangxi Key Laboratory of Plant Functional Phytochemicals and Sustainable Utilization, Guangxi Institute of Botany, Guangxi Zhuang Autonomous Region and Chinese Academy of Sciences, Guilin 541006, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, ChinaGuilin GFS Monk Fruit Corp, Guilin 541006, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, ChinaInstitute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100193, ChinaMogrosides are a group of health-promoting natural products that extracted from <i>Siraitia grosvenorii</i> fruit (Luo-han-guo or monk fruit), which exhibited a promising practical application in natural sweeteners and pharmaceutical development. However, the production of mogrosides is inadequate to meet the need worldwide, and uneconomical synthetic chemistry methods are not generally recommended for structural complexity. To address this issue, an in-fusion based gene stacking strategy (IGS) for multigene stacking has been developed to assemble 6 mogrosides synthase genes in pCAMBIA1300. Metabolic engineering of <i>Nicotiana benthamiana</i> and <i>Arabidopsis thaliana</i> to produce mogrosides from 2,3-oxidosqualene was carried out. Moreover, a validated HPLC-MS/MS method was used for the quantitative analysis of mogrosides in transgenic plants. Herein, engineered <i>Arabidopsis thaliana</i> produced siamenoside I ranging from 29.65 to 1036.96 ng/g FW, and the content of mogroside III at 202.75 ng/g FW, respectively. The production of mogroside III was from 148.30 to 252.73 ng/g FW, and mogroside II-E with concentration between 339.27 and 5663.55 ng/g FW in the engineered tobacco, respectively. This study provides information potentially applicable to develop a powerful and green toolkit for the production of mogrosides.https://www.mdpi.com/1422-0067/23/18/10422mogrosidesnatural sweetenerplant chassismultigene assemblysynthetic biology |
| spellingShingle | Jingjing Liao Tingyao Liu Lei Xie Changming Mo Xiyang Huang Shengrong Cui Xunli Jia Fusheng Lan Zuliang Luo Xiaojun Ma Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides International Journal of Molecular Sciences mogrosides natural sweetener plant chassis multigene assembly synthetic biology |
| title | Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides |
| title_full | Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides |
| title_fullStr | Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides |
| title_full_unstemmed | Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides |
| title_short | Plant Metabolic Engineering by Multigene Stacking: Synthesis of Diverse Mogrosides |
| title_sort | plant metabolic engineering by multigene stacking synthesis of diverse mogrosides |
| topic | mogrosides natural sweetener plant chassis multigene assembly synthetic biology |
| url | https://www.mdpi.com/1422-0067/23/18/10422 |
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