Undisturbed Soil Pedon under Birch Forest: Characterization of Microbiome in Genetic Horizons
Vast areas of land in the forest-steppe of West Siberia are occupied by birch forests, the most common ecosystems there. However, currently, little is known about the microbiome composition in the underlying soil, especially along a sequence of soil genetic horizons. The study aimed at inventorying...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Soil Systems |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2571-8789/5/1/14 |
| _version_ | 1797395232854114304 |
|---|---|
| author | Natalia B. Naumova Ivan P. Belanov Tatiana Y. Alikina Marsel R. Kabilov |
| author_facet | Natalia B. Naumova Ivan P. Belanov Tatiana Y. Alikina Marsel R. Kabilov |
| author_sort | Natalia B. Naumova |
| collection | DOAJ |
| description | Vast areas of land in the forest-steppe of West Siberia are occupied by birch forests, the most common ecosystems there. However, currently, little is known about the microbiome composition in the underlying soil, especially along a sequence of soil genetic horizons. The study aimed at inventorying microbiome in genetic horizons of a typical Phaeozem under undisturbed birch forest in West Siberia. Bacteria and fungi were studied using 16S rRNA genes’ and ITS2 amplicon sequencing with Illumina MiSeq. <i>Proteobacteria</i> and <i>Acidobacteria</i> together accounted for two-thirds of the operational taxonomic units (OTUs) numbers and half of the sequences in each genetic horizon. <i>Acidobacteria</i> predominated in eluvial environments, whereas <i>Proteobacteria</i>, preferred topsoil. The fungal sequences were dominated by <i>Ascomycota</i> and <i>Basidiomycota</i> phyla. <i>Basidiomycota</i> was the most abundant in the topsoil, whereas <i>Ascomycota</i> increased down the soil profile. <i>Thelephoraceae</i> family was the most abundant in the A horizon, whereas the <i>Pyronemataceae</i> family dominants in the AEl horizon, ultimately prevailing in the subsoil. We conclude that soil genetic horizons shape distinct microbiomes, therefore soil horizontation should be accounted for while studying undisturbed soils. This study, representing the first description of bacterio- and mycobiomes in genetic horizons of the Phaeozem profile, provides a reference for future research. |
| first_indexed | 2024-03-09T00:31:27Z |
| format | Article |
| id | doaj.art-6ca3881077f945ae8fc18296b14af132 |
| institution | Directory Open Access Journal |
| issn | 2571-8789 |
| language | English |
| last_indexed | 2024-03-09T00:31:27Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Soil Systems |
| spelling | doaj.art-6ca3881077f945ae8fc18296b14af1322023-12-11T18:28:16ZengMDPI AGSoil Systems2571-87892021-02-01511410.3390/soilsystems5010014Undisturbed Soil Pedon under Birch Forest: Characterization of Microbiome in Genetic HorizonsNatalia B. Naumova0Ivan P. Belanov1Tatiana Y. Alikina2Marsel R. Kabilov3Institute of Soil Science and Agrochemistry SB RAS, Lavrentieva 8/2, 630090 Novosibirsk, RussiaInstitute of Soil Science and Agrochemistry SB RAS, Lavrentieva 8/2, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentieva 8, 630090 Novosibirsk, RussiaInstitute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentieva 8, 630090 Novosibirsk, RussiaVast areas of land in the forest-steppe of West Siberia are occupied by birch forests, the most common ecosystems there. However, currently, little is known about the microbiome composition in the underlying soil, especially along a sequence of soil genetic horizons. The study aimed at inventorying microbiome in genetic horizons of a typical Phaeozem under undisturbed birch forest in West Siberia. Bacteria and fungi were studied using 16S rRNA genes’ and ITS2 amplicon sequencing with Illumina MiSeq. <i>Proteobacteria</i> and <i>Acidobacteria</i> together accounted for two-thirds of the operational taxonomic units (OTUs) numbers and half of the sequences in each genetic horizon. <i>Acidobacteria</i> predominated in eluvial environments, whereas <i>Proteobacteria</i>, preferred topsoil. The fungal sequences were dominated by <i>Ascomycota</i> and <i>Basidiomycota</i> phyla. <i>Basidiomycota</i> was the most abundant in the topsoil, whereas <i>Ascomycota</i> increased down the soil profile. <i>Thelephoraceae</i> family was the most abundant in the A horizon, whereas the <i>Pyronemataceae</i> family dominants in the AEl horizon, ultimately prevailing in the subsoil. We conclude that soil genetic horizons shape distinct microbiomes, therefore soil horizontation should be accounted for while studying undisturbed soils. This study, representing the first description of bacterio- and mycobiomes in genetic horizons of the Phaeozem profile, provides a reference for future research.https://www.mdpi.com/2571-8789/5/1/14ITS DNA diversity16S rRNA gene diversityPhaeozemsoil propertiesforest-steppe |
| spellingShingle | Natalia B. Naumova Ivan P. Belanov Tatiana Y. Alikina Marsel R. Kabilov Undisturbed Soil Pedon under Birch Forest: Characterization of Microbiome in Genetic Horizons Soil Systems ITS DNA diversity 16S rRNA gene diversity Phaeozem soil properties forest-steppe |
| title | Undisturbed Soil Pedon under Birch Forest: Characterization of Microbiome in Genetic Horizons |
| title_full | Undisturbed Soil Pedon under Birch Forest: Characterization of Microbiome in Genetic Horizons |
| title_fullStr | Undisturbed Soil Pedon under Birch Forest: Characterization of Microbiome in Genetic Horizons |
| title_full_unstemmed | Undisturbed Soil Pedon under Birch Forest: Characterization of Microbiome in Genetic Horizons |
| title_short | Undisturbed Soil Pedon under Birch Forest: Characterization of Microbiome in Genetic Horizons |
| title_sort | undisturbed soil pedon under birch forest characterization of microbiome in genetic horizons |
| topic | ITS DNA diversity 16S rRNA gene diversity Phaeozem soil properties forest-steppe |
| url | https://www.mdpi.com/2571-8789/5/1/14 |
| work_keys_str_mv | AT nataliabnaumova undisturbedsoilpedonunderbirchforestcharacterizationofmicrobiomeingenetichorizons AT ivanpbelanov undisturbedsoilpedonunderbirchforestcharacterizationofmicrobiomeingenetichorizons AT tatianayalikina undisturbedsoilpedonunderbirchforestcharacterizationofmicrobiomeingenetichorizons AT marselrkabilov undisturbedsoilpedonunderbirchforestcharacterizationofmicrobiomeingenetichorizons |