Virus load and clinical features during the acute phase of Chikungunya infection in children.
BACKGROUND:Chikungunya virus (CHIKV) infection is a long known mosquito-borne disease that is associated with severe morbidity, characterized by fever, headache, rashes, joint pain, and myalgia. It is believed that virus load has relation with severity of clinical features. OBJECTIVES:We performed t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0211036 |
| _version_ | 1818571872795623424 |
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| author | Siva Raghavendhar B Ashok Kumar Patel Sushil Kumar Kabra Rakesh Lodha Vinod H Ratageri Pratima Ray |
| author_facet | Siva Raghavendhar B Ashok Kumar Patel Sushil Kumar Kabra Rakesh Lodha Vinod H Ratageri Pratima Ray |
| author_sort | Siva Raghavendhar B |
| collection | DOAJ |
| description | BACKGROUND:Chikungunya virus (CHIKV) infection is a long known mosquito-borne disease that is associated with severe morbidity, characterized by fever, headache, rashes, joint pain, and myalgia. It is believed that virus load has relation with severity of clinical features. OBJECTIVES:We performed this study to assess the relationship between virus load and clinical features in children during the acute phase of CHIKV infection, in order to draw insights for better-informed treatment. STUDY DESIGN:Between June 1, 2009, and May 31, 2010, 338 patients with fever and susceptive to CHIKV during first 4 days of illness were prospectively enrolled from Karnataka Institute of Medical Sciences, Hubli in our hospital based cross sectional observational study. Sybr green quantitative reverse transcription polymerase chain reaction was performed to estimate the virus load. RESULTS:Quantitative RT-PCR was positive for CHIKV in 54 patients. The median copy number of CHIKV was 1.3x 108 copies/ml (1.7x105-9.9x109 copies/ml). Among the observed clinical features, a statistically significant difference in log mean virus load was found between patients with and without myalgia (log mean 7.50 vs 8.34, P = 0.01). CONCLUSION:Patients with myalgia had lower virus load and those without myalgia had a higher virus load. |
| first_indexed | 2024-12-14T18:50:00Z |
| format | Article |
| id | doaj.art-6ca58a97e0b0416a83265b70a3c533fd |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T18:50:00Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6ca58a97e0b0416a83265b70a3c533fd2022-12-21T22:51:17ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01142e021103610.1371/journal.pone.0211036Virus load and clinical features during the acute phase of Chikungunya infection in children.Siva Raghavendhar BAshok Kumar PatelSushil Kumar KabraRakesh LodhaVinod H RatageriPratima RayBACKGROUND:Chikungunya virus (CHIKV) infection is a long known mosquito-borne disease that is associated with severe morbidity, characterized by fever, headache, rashes, joint pain, and myalgia. It is believed that virus load has relation with severity of clinical features. OBJECTIVES:We performed this study to assess the relationship between virus load and clinical features in children during the acute phase of CHIKV infection, in order to draw insights for better-informed treatment. STUDY DESIGN:Between June 1, 2009, and May 31, 2010, 338 patients with fever and susceptive to CHIKV during first 4 days of illness were prospectively enrolled from Karnataka Institute of Medical Sciences, Hubli in our hospital based cross sectional observational study. Sybr green quantitative reverse transcription polymerase chain reaction was performed to estimate the virus load. RESULTS:Quantitative RT-PCR was positive for CHIKV in 54 patients. The median copy number of CHIKV was 1.3x 108 copies/ml (1.7x105-9.9x109 copies/ml). Among the observed clinical features, a statistically significant difference in log mean virus load was found between patients with and without myalgia (log mean 7.50 vs 8.34, P = 0.01). CONCLUSION:Patients with myalgia had lower virus load and those without myalgia had a higher virus load.https://doi.org/10.1371/journal.pone.0211036 |
| spellingShingle | Siva Raghavendhar B Ashok Kumar Patel Sushil Kumar Kabra Rakesh Lodha Vinod H Ratageri Pratima Ray Virus load and clinical features during the acute phase of Chikungunya infection in children. PLoS ONE |
| title | Virus load and clinical features during the acute phase of Chikungunya infection in children. |
| title_full | Virus load and clinical features during the acute phase of Chikungunya infection in children. |
| title_fullStr | Virus load and clinical features during the acute phase of Chikungunya infection in children. |
| title_full_unstemmed | Virus load and clinical features during the acute phase of Chikungunya infection in children. |
| title_short | Virus load and clinical features during the acute phase of Chikungunya infection in children. |
| title_sort | virus load and clinical features during the acute phase of chikungunya infection in children |
| url | https://doi.org/10.1371/journal.pone.0211036 |
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