Deficiency of mastl, a mitotic regulator, results in cell detachment from developing tissues of zebrafish embryos
To form tissues with unique functions and structures, it is important that the cells that comprise them maintain physical contact. On the other hand, with each mitosis, drastic changes in cell shapes, cell adhesion, and cytoskeletal architecture may cause such contacts to be temporarily weakened, ri...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2024.1375655/full |
| _version_ | 1797266428798173184 |
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| author | Hideko Utsumi Hideko Utsumi Taijiro Yabe Taijiro Yabe Taijiro Yabe Sumito Koshida Sumito Koshida Sumito Koshida Akira Yamashita Akira Yamashita Akira Yamashita Shinji Takada Shinji Takada Shinji Takada |
| author_facet | Hideko Utsumi Hideko Utsumi Taijiro Yabe Taijiro Yabe Taijiro Yabe Sumito Koshida Sumito Koshida Sumito Koshida Akira Yamashita Akira Yamashita Akira Yamashita Shinji Takada Shinji Takada Shinji Takada |
| author_sort | Hideko Utsumi |
| collection | DOAJ |
| description | To form tissues with unique functions and structures, it is important that the cells that comprise them maintain physical contact. On the other hand, with each mitosis, drastic changes in cell shapes, cell adhesion, and cytoskeletal architecture may cause such contacts to be temporarily weakened, risking improper development and maintenance of tissues. Despite such risks, tissues form properly during normal development. However, it is not well understood whether mitotic abnormalities affect tissue formation. Here, analysis of zebrafish embryos with aberrant mitosis shows that proper progression of mitosis is important to maintain cell contact in developing tissues. By screening mutants with abnormal trunk and tail development, we obtained a mutant with perturbed expression of some tissue-specific genes in embryonic caudal regions. The responsible gene is mastl/gwl, which is involved in progression of mitosis. Analysis focusing on the chordo-neural hinge (CNH), the primordium of axial tissues, shows that cell detachment from the CNH is increased in mastl mutant embryos. Time-lapse imaging reveals that this cell detachment occurs during mitosis. These results suggest that cells are unable to maintain contact due to abnormalities in progression of mitosis in mastl mutants. |
| first_indexed | 2024-04-25T01:00:32Z |
| format | Article |
| id | doaj.art-6ca67af4c2a14a9f8c382e7816720fb0 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-25T01:00:32Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-6ca67af4c2a14a9f8c382e7816720fb02024-03-11T05:03:41ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2024-03-011210.3389/fcell.2024.13756551375655Deficiency of mastl, a mitotic regulator, results in cell detachment from developing tissues of zebrafish embryosHideko Utsumi0Hideko Utsumi1Taijiro Yabe2Taijiro Yabe3Taijiro Yabe4Sumito Koshida5Sumito Koshida6Sumito Koshida7Akira Yamashita8Akira Yamashita9Akira Yamashita10Shinji Takada11Shinji Takada12Shinji Takada13National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, JapanExploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Aichi, JapanNational Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, JapanExploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Aichi, JapanThe Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, JapanNational Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, JapanThe Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, JapanShumei University, Yachiyo, Chiba, JapanNational Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, JapanThe Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, JapanGraduate School of Arts and Science, The university of Tokyo, Tokyo, JapanNational Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi, JapanExploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Aichi, JapanThe Graduate University for Advanced Studies (SOKENDAI), Okazaki, Aichi, JapanTo form tissues with unique functions and structures, it is important that the cells that comprise them maintain physical contact. On the other hand, with each mitosis, drastic changes in cell shapes, cell adhesion, and cytoskeletal architecture may cause such contacts to be temporarily weakened, risking improper development and maintenance of tissues. Despite such risks, tissues form properly during normal development. However, it is not well understood whether mitotic abnormalities affect tissue formation. Here, analysis of zebrafish embryos with aberrant mitosis shows that proper progression of mitosis is important to maintain cell contact in developing tissues. By screening mutants with abnormal trunk and tail development, we obtained a mutant with perturbed expression of some tissue-specific genes in embryonic caudal regions. The responsible gene is mastl/gwl, which is involved in progression of mitosis. Analysis focusing on the chordo-neural hinge (CNH), the primordium of axial tissues, shows that cell detachment from the CNH is increased in mastl mutant embryos. Time-lapse imaging reveals that this cell detachment occurs during mitosis. These results suggest that cells are unable to maintain contact due to abnormalities in progression of mitosis in mastl mutants.https://www.frontiersin.org/articles/10.3389/fcell.2024.1375655/fullMASTLmitosiszebrafishaxis elongationchordo-neural hingecell detachment |
| spellingShingle | Hideko Utsumi Hideko Utsumi Taijiro Yabe Taijiro Yabe Taijiro Yabe Sumito Koshida Sumito Koshida Sumito Koshida Akira Yamashita Akira Yamashita Akira Yamashita Shinji Takada Shinji Takada Shinji Takada Deficiency of mastl, a mitotic regulator, results in cell detachment from developing tissues of zebrafish embryos Frontiers in Cell and Developmental Biology MASTL mitosis zebrafish axis elongation chordo-neural hinge cell detachment |
| title | Deficiency of mastl, a mitotic regulator, results in cell detachment from developing tissues of zebrafish embryos |
| title_full | Deficiency of mastl, a mitotic regulator, results in cell detachment from developing tissues of zebrafish embryos |
| title_fullStr | Deficiency of mastl, a mitotic regulator, results in cell detachment from developing tissues of zebrafish embryos |
| title_full_unstemmed | Deficiency of mastl, a mitotic regulator, results in cell detachment from developing tissues of zebrafish embryos |
| title_short | Deficiency of mastl, a mitotic regulator, results in cell detachment from developing tissues of zebrafish embryos |
| title_sort | deficiency of mastl a mitotic regulator results in cell detachment from developing tissues of zebrafish embryos |
| topic | MASTL mitosis zebrafish axis elongation chordo-neural hinge cell detachment |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2024.1375655/full |
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