Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae)
ABSTRACT Wasps are a diverse group of insects that possess a sting apparatus associated with a venom gland, which is used for predation and colony defense. The biochemistry of Hymenoptera venom has been evaluated in relation to allergy and immunology, and proteomics has been shown to be a powerful t...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Entomologia
2020-01-01
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| Series: | Revista Brasileira de Entomologia |
| Subjects: | |
| Online Access: | http://www.scielo.br/pdf/rbent/v63n4/0085-5626-rbent-63-04-0322.pdf |
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| author | Angélica Mendonça Rafaella C. Bernardi Marchiotti Ellen L.B. Firmino Pollyanna P. Santos Denise Sguarizi Antonio José E. Serrão Claudia A.L. Cardoso William F. Antonialli Junior |
| author_facet | Angélica Mendonça Rafaella C. Bernardi Marchiotti Ellen L.B. Firmino Pollyanna P. Santos Denise Sguarizi Antonio José E. Serrão Claudia A.L. Cardoso William F. Antonialli Junior |
| author_sort | Angélica Mendonça |
| collection | DOAJ |
| description | ABSTRACT Wasps are a diverse group of insects that possess a sting apparatus associated with a venom gland, which is used for predation and colony defense. The biochemistry of Hymenoptera venom has been evaluated in relation to allergy and immunology, and proteomics has been shown to be a powerful tool for the identification of compounds with pharmacological potential. Data on wasps venom the of genus Apoica are scarce, so the objective of the present work was to identify the venom proteins of the eusocial wasp Apoica pallens, as a first step towards further investigation of applied uses of the venom and its protein constituents. The venom proteins were separated by two-dimensional gel electrophoresis, followed by MALDI-TOF/TOF mass spectrometry. A total of 259 spots were detected, with molecular weights from 4.9 to 141 kDa. Thirty of these proteins were identified and classified into eight functional categories: allergen, enzyme, metabolism, structural, environmental response, proteoglycan, active in DNA and RNA, and unknown function. Due to the few available proteomic data for wasp venom, many proteins could not be identified, which makes studies with proteomic analysis of Hymenoptera venom even more important. |
| first_indexed | 2024-04-11T20:29:15Z |
| format | Article |
| id | doaj.art-6ca7a533de564e5f8caf8bb3dc251e41 |
| institution | Directory Open Access Journal |
| issn | 1806-9665 |
| language | English |
| last_indexed | 2024-04-11T20:29:15Z |
| publishDate | 2020-01-01 |
| publisher | Sociedade Brasileira de Entomologia |
| record_format | Article |
| series | Revista Brasileira de Entomologia |
| spelling | doaj.art-6ca7a533de564e5f8caf8bb3dc251e412022-12-22T04:04:33ZengSociedade Brasileira de EntomologiaRevista Brasileira de Entomologia1806-96652020-01-0163432233010.1016/j.rbe.2019.10.001Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae)Angélica Mendonçahttps://orcid.org/0000-0001-5778-4406Rafaella C. Bernardi MarchiottiEllen L.B. FirminoPollyanna P. SantosDenise Sguarizi AntonioJosé E. SerrãoClaudia A.L. CardosoWilliam F. Antonialli JuniorABSTRACT Wasps are a diverse group of insects that possess a sting apparatus associated with a venom gland, which is used for predation and colony defense. The biochemistry of Hymenoptera venom has been evaluated in relation to allergy and immunology, and proteomics has been shown to be a powerful tool for the identification of compounds with pharmacological potential. Data on wasps venom the of genus Apoica are scarce, so the objective of the present work was to identify the venom proteins of the eusocial wasp Apoica pallens, as a first step towards further investigation of applied uses of the venom and its protein constituents. The venom proteins were separated by two-dimensional gel electrophoresis, followed by MALDI-TOF/TOF mass spectrometry. A total of 259 spots were detected, with molecular weights from 4.9 to 141 kDa. Thirty of these proteins were identified and classified into eight functional categories: allergen, enzyme, metabolism, structural, environmental response, proteoglycan, active in DNA and RNA, and unknown function. Due to the few available proteomic data for wasp venom, many proteins could not be identified, which makes studies with proteomic analysis of Hymenoptera venom even more important.http://www.scielo.br/pdf/rbent/v63n4/0085-5626-rbent-63-04-0322.pdfMass spectrometryMALDI-TOF/TOFProteinsWasps |
| spellingShingle | Angélica Mendonça Rafaella C. Bernardi Marchiotti Ellen L.B. Firmino Pollyanna P. Santos Denise Sguarizi Antonio José E. Serrão Claudia A.L. Cardoso William F. Antonialli Junior Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae) Revista Brasileira de Entomologia Mass spectrometry MALDI-TOF/TOF Proteins Wasps |
| title | Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae) |
| title_full | Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae) |
| title_fullStr | Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae) |
| title_full_unstemmed | Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae) |
| title_short | Proteomic analysis of the venom of the social wasp Apoica pallens (Hymenoptera: Vespidae) |
| title_sort | proteomic analysis of the venom of the social wasp apoica pallens hymenoptera vespidae |
| topic | Mass spectrometry MALDI-TOF/TOF Proteins Wasps |
| url | http://www.scielo.br/pdf/rbent/v63n4/0085-5626-rbent-63-04-0322.pdf |
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