Characteristics of Hepatitis B virus integration and mechanism of inducing chromosome translocation
Abstract Hepatitis B virus (HBV) integration is closely associated with the onset and progression of tumors. This study utilized the DNA of 27 liver cancer samples for high-throughput Viral Integration Detection (HIVID), with the overarching goal of detecting HBV integration. KEGG pathway analysis o...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-06-01
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| Series: | npj Genomic Medicine |
| Online Access: | https://doi.org/10.1038/s41525-023-00355-y |
| _version_ | 1797811341966180352 |
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| author | Xiaofang Cui Yiyan Li Hanshi Xu Yuhui Sun Shulong Jiang Weiyang Li |
| author_facet | Xiaofang Cui Yiyan Li Hanshi Xu Yuhui Sun Shulong Jiang Weiyang Li |
| author_sort | Xiaofang Cui |
| collection | DOAJ |
| description | Abstract Hepatitis B virus (HBV) integration is closely associated with the onset and progression of tumors. This study utilized the DNA of 27 liver cancer samples for high-throughput Viral Integration Detection (HIVID), with the overarching goal of detecting HBV integration. KEGG pathway analysis of breakpoints was performed using the ClusterProfiler software. The breakpoints were annotated using the latest ANNOVAR software. We identified 775 integration sites and detected two new hotspot genes for virus integration, N4BP1 and WASHP, along with 331 new genes. Furthermore, we conducted a comprehensive analysis to determine the critical impact pathways of virus integration by combining our findings with the results of three major global studies on HBV integration. Meanwhile, we found common characteristics of virus integration hotspots among different ethnic groups. To specify the direct impact of virus integration on genomic instability, we explained the causes of inversion and the frequent occurrence of translocation due to HBV integration. This study detected a series of hotspot integration genes and specified common characteristics of critical hotspot integration genes. These hotspot genes are universal across different ethnic groups, providing an effective target for better research on the pathogenic mechanism. We also demonstrated more comprehensive key pathways affected by HBV integration and elucidated the mechanism for inversion and frequent translocation events due to virus integration. Apart from the great significance of the rule of HBV integration, the current study also provides valuable insights into the mechanism of virus integration. |
| first_indexed | 2024-03-13T07:22:16Z |
| format | Article |
| id | doaj.art-6ca8f89315c04a6db801f7a72637f473 |
| institution | Directory Open Access Journal |
| issn | 2056-7944 |
| language | English |
| last_indexed | 2024-03-13T07:22:16Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Genomic Medicine |
| spelling | doaj.art-6ca8f89315c04a6db801f7a72637f4732023-06-04T11:35:09ZengNature Portfolionpj Genomic Medicine2056-79442023-06-01811710.1038/s41525-023-00355-yCharacteristics of Hepatitis B virus integration and mechanism of inducing chromosome translocationXiaofang Cui0Yiyan Li1Hanshi Xu2Yuhui Sun3Shulong Jiang4Weiyang Li5Jining Medical UniversityCollege of Life Sciences, University of Chinese Academy of SciencesInstitute of Microbiology, Chinese Academy of SciencesBGI-ShenzhenClinical Medical Laboratory Center, Jining First People’s Hospital, Shandong First Medical UniversityJining Medical UniversityAbstract Hepatitis B virus (HBV) integration is closely associated with the onset and progression of tumors. This study utilized the DNA of 27 liver cancer samples for high-throughput Viral Integration Detection (HIVID), with the overarching goal of detecting HBV integration. KEGG pathway analysis of breakpoints was performed using the ClusterProfiler software. The breakpoints were annotated using the latest ANNOVAR software. We identified 775 integration sites and detected two new hotspot genes for virus integration, N4BP1 and WASHP, along with 331 new genes. Furthermore, we conducted a comprehensive analysis to determine the critical impact pathways of virus integration by combining our findings with the results of three major global studies on HBV integration. Meanwhile, we found common characteristics of virus integration hotspots among different ethnic groups. To specify the direct impact of virus integration on genomic instability, we explained the causes of inversion and the frequent occurrence of translocation due to HBV integration. This study detected a series of hotspot integration genes and specified common characteristics of critical hotspot integration genes. These hotspot genes are universal across different ethnic groups, providing an effective target for better research on the pathogenic mechanism. We also demonstrated more comprehensive key pathways affected by HBV integration and elucidated the mechanism for inversion and frequent translocation events due to virus integration. Apart from the great significance of the rule of HBV integration, the current study also provides valuable insights into the mechanism of virus integration.https://doi.org/10.1038/s41525-023-00355-y |
| spellingShingle | Xiaofang Cui Yiyan Li Hanshi Xu Yuhui Sun Shulong Jiang Weiyang Li Characteristics of Hepatitis B virus integration and mechanism of inducing chromosome translocation npj Genomic Medicine |
| title | Characteristics of Hepatitis B virus integration and mechanism of inducing chromosome translocation |
| title_full | Characteristics of Hepatitis B virus integration and mechanism of inducing chromosome translocation |
| title_fullStr | Characteristics of Hepatitis B virus integration and mechanism of inducing chromosome translocation |
| title_full_unstemmed | Characteristics of Hepatitis B virus integration and mechanism of inducing chromosome translocation |
| title_short | Characteristics of Hepatitis B virus integration and mechanism of inducing chromosome translocation |
| title_sort | characteristics of hepatitis b virus integration and mechanism of inducing chromosome translocation |
| url | https://doi.org/10.1038/s41525-023-00355-y |
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