Optical Fiber-Based Recording of Climbing Fiber Ca<sup>2+</sup> Signals in Freely Behaving Mice
The olivocerebellar circuitry is important to convey both motor and non-motor information from the inferior olive (IO) to the cerebellar cortex. Several methods are currently established to observe the dynamics of the olivocerebellar circuitry, largely by recording the complex spike activity of cere...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/11/6/907 |
| _version_ | 1797489801228713984 |
|---|---|
| author | Jiechang Tang Rou Xue Yan Wang Min Li Hongbo Jia Janelle M. P. Pakan Longhui Li Xiaowei Chen Xingyi Li |
| author_facet | Jiechang Tang Rou Xue Yan Wang Min Li Hongbo Jia Janelle M. P. Pakan Longhui Li Xiaowei Chen Xingyi Li |
| author_sort | Jiechang Tang |
| collection | DOAJ |
| description | The olivocerebellar circuitry is important to convey both motor and non-motor information from the inferior olive (IO) to the cerebellar cortex. Several methods are currently established to observe the dynamics of the olivocerebellar circuitry, largely by recording the complex spike activity of cerebellar Purkinje cells; however, these techniques can be technically challenging to apply in vivo and are not always possible in freely behaving animals. Here, we developed a method for the direct, accessible, and robust recording of climbing fiber (CF) Ca<sup>2+</sup> signals based on optical fiber photometry. We first verified the IO stereotactic coordinates and the organization of contralateral CF projections using tracing techniques and then injected Ca<sup>2+</sup> indicators optimized for axonal labeling, followed by optical fiber-based recordings. We demonstrated this method by recording CF Ca<sup>2+</sup> signals in lobule IV/V of the cerebellar vermis, comparing the resulting signals in freely moving mice. We found various movement-evoked CF Ca<sup>2+</sup> signals, but the onset of exploratory-like behaviors, including rearing and tiptoe standing, was highly synchronous with recorded CF activity. Thus, we have successfully established a robust and accessible method to record the CF Ca<sup>2+</sup> signals in freely behaving mice, which will extend the toolbox for studying cerebellar function and related disorders. |
| first_indexed | 2024-03-10T00:22:49Z |
| format | Article |
| id | doaj.art-6ca95aab7d3049c08739224b660cb7b6 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T00:22:49Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-6ca95aab7d3049c08739224b660cb7b62023-11-23T15:40:18ZengMDPI AGBiology2079-77372022-06-0111690710.3390/biology11060907Optical Fiber-Based Recording of Climbing Fiber Ca<sup>2+</sup> Signals in Freely Behaving MiceJiechang Tang0Rou Xue1Yan Wang2Min Li3Hongbo Jia4Janelle M. P. Pakan5Longhui Li6Xiaowei Chen7Xingyi Li8Center for Neurointelligence, School of Medicine, Chongqing University, Chongqing 400030, ChinaCenter for Neurointelligence, School of Medicine, Chongqing University, Chongqing 400030, ChinaBrain Research Instrument Innovation Center, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaBrain Research Instrument Innovation Center, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaBrain Research Instrument Innovation Center, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, ChinaInstitute of Cognitive Neurology and Dementia Research, Otto-von-Guericke University, 39120 Magdeburg, GermanyCenter for Neurointelligence, School of Medicine, Chongqing University, Chongqing 400030, ChinaBrain Research Center and State Key Laboratory of Trauma, Burns, and Combined Injury, Third Military Medical University, Chongqing 400038, ChinaCenter for Neurointelligence, School of Medicine, Chongqing University, Chongqing 400030, ChinaThe olivocerebellar circuitry is important to convey both motor and non-motor information from the inferior olive (IO) to the cerebellar cortex. Several methods are currently established to observe the dynamics of the olivocerebellar circuitry, largely by recording the complex spike activity of cerebellar Purkinje cells; however, these techniques can be technically challenging to apply in vivo and are not always possible in freely behaving animals. Here, we developed a method for the direct, accessible, and robust recording of climbing fiber (CF) Ca<sup>2+</sup> signals based on optical fiber photometry. We first verified the IO stereotactic coordinates and the organization of contralateral CF projections using tracing techniques and then injected Ca<sup>2+</sup> indicators optimized for axonal labeling, followed by optical fiber-based recordings. We demonstrated this method by recording CF Ca<sup>2+</sup> signals in lobule IV/V of the cerebellar vermis, comparing the resulting signals in freely moving mice. We found various movement-evoked CF Ca<sup>2+</sup> signals, but the onset of exploratory-like behaviors, including rearing and tiptoe standing, was highly synchronous with recorded CF activity. Thus, we have successfully established a robust and accessible method to record the CF Ca<sup>2+</sup> signals in freely behaving mice, which will extend the toolbox for studying cerebellar function and related disorders.https://www.mdpi.com/2079-7737/11/6/907the olivocerebellar circuitryclimbing fibersoptical fiber photometrylobule IV/V of the cerebellar vermisopen field |
| spellingShingle | Jiechang Tang Rou Xue Yan Wang Min Li Hongbo Jia Janelle M. P. Pakan Longhui Li Xiaowei Chen Xingyi Li Optical Fiber-Based Recording of Climbing Fiber Ca<sup>2+</sup> Signals in Freely Behaving Mice Biology the olivocerebellar circuitry climbing fibers optical fiber photometry lobule IV/V of the cerebellar vermis open field |
| title | Optical Fiber-Based Recording of Climbing Fiber Ca<sup>2+</sup> Signals in Freely Behaving Mice |
| title_full | Optical Fiber-Based Recording of Climbing Fiber Ca<sup>2+</sup> Signals in Freely Behaving Mice |
| title_fullStr | Optical Fiber-Based Recording of Climbing Fiber Ca<sup>2+</sup> Signals in Freely Behaving Mice |
| title_full_unstemmed | Optical Fiber-Based Recording of Climbing Fiber Ca<sup>2+</sup> Signals in Freely Behaving Mice |
| title_short | Optical Fiber-Based Recording of Climbing Fiber Ca<sup>2+</sup> Signals in Freely Behaving Mice |
| title_sort | optical fiber based recording of climbing fiber ca sup 2 sup signals in freely behaving mice |
| topic | the olivocerebellar circuitry climbing fibers optical fiber photometry lobule IV/V of the cerebellar vermis open field |
| url | https://www.mdpi.com/2079-7737/11/6/907 |
| work_keys_str_mv | AT jiechangtang opticalfiberbasedrecordingofclimbingfibercasup2supsignalsinfreelybehavingmice AT rouxue opticalfiberbasedrecordingofclimbingfibercasup2supsignalsinfreelybehavingmice AT yanwang opticalfiberbasedrecordingofclimbingfibercasup2supsignalsinfreelybehavingmice AT minli opticalfiberbasedrecordingofclimbingfibercasup2supsignalsinfreelybehavingmice AT hongbojia opticalfiberbasedrecordingofclimbingfibercasup2supsignalsinfreelybehavingmice AT janellemppakan opticalfiberbasedrecordingofclimbingfibercasup2supsignalsinfreelybehavingmice AT longhuili opticalfiberbasedrecordingofclimbingfibercasup2supsignalsinfreelybehavingmice AT xiaoweichen opticalfiberbasedrecordingofclimbingfibercasup2supsignalsinfreelybehavingmice AT xingyili opticalfiberbasedrecordingofclimbingfibercasup2supsignalsinfreelybehavingmice |