Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes
The random amplified polymorphic DNA (RAPD) technique was used to evaluate both genetic diversity among 19 soybean accessions included in the Czech National Collection of Soybean Genotypes and their potential as a new source of genetic variations for soybean breeding programs. Only 22 of all the 40...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Czech Academy of Agricultural Sciences
2002-06-01
|
| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-200202-0002_evaluation-of-genetic-diversity-in-19-glycine-max-l-merr-accessions-included-in-the-czech-national-collecti.php |
| _version_ | 1797899541400256512 |
|---|---|
| author | M. Baránek M. Kadlec J. Raddová M. Vachůn M. Pidra |
| author_facet | M. Baránek M. Kadlec J. Raddová M. Vachůn M. Pidra |
| author_sort | M. Baránek |
| collection | DOAJ |
| description | The random amplified polymorphic DNA (RAPD) technique was used to evaluate both genetic diversity among 19 soybean accessions included in the Czech National Collection of Soybean Genotypes and their potential as a new source of genetic variations for soybean breeding programs. Only 22 of all the 40 random primers used in RAPD reactions showed polymorphism acceptable for an effective characterisation of these accessions. Altogether 122 highly reproducible RAPD fragments were generated, 55 of them were polymorphic (46%). However, because of the previously observed low degree of RAPD polymorphism in the case of Glycine max, fragments with low level of informativeness were evaluated, too. Presented results enable the selection of genetically distinct individuals. Such information may be useful to breeders willing to use genetically diverse introductions in soybean improvement process. |
| first_indexed | 2024-04-10T08:31:35Z |
| format | Article |
| id | doaj.art-6cab51dc1aad4dcfa828d3d657a58f6d |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:31:35Z |
| publishDate | 2002-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-6cab51dc1aad4dcfa828d3d657a58f6d2023-02-23T03:28:54ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252002-06-01382697410.17221/6114-CJGPBcjg-200202-0002Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypesM. Baránek0M. Kadlec1J. Raddová2M. Vachůn3M. Pidra4Mendeleum, Horticultural Faculty, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech RepublicMendeleum, Horticultural Faculty, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech RepublicMendeleum, Horticultural Faculty, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech RepublicMendeleum, Horticultural Faculty, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech RepublicMendeleum, Horticultural Faculty, Mendel University of Agriculture and Forestry in Brno, Lednice na Moravě, Czech RepublicThe random amplified polymorphic DNA (RAPD) technique was used to evaluate both genetic diversity among 19 soybean accessions included in the Czech National Collection of Soybean Genotypes and their potential as a new source of genetic variations for soybean breeding programs. Only 22 of all the 40 random primers used in RAPD reactions showed polymorphism acceptable for an effective characterisation of these accessions. Altogether 122 highly reproducible RAPD fragments were generated, 55 of them were polymorphic (46%). However, because of the previously observed low degree of RAPD polymorphism in the case of Glycine max, fragments with low level of informativeness were evaluated, too. Presented results enable the selection of genetically distinct individuals. Such information may be useful to breeders willing to use genetically diverse introductions in soybean improvement process.https://cjgpb.agriculturejournals.cz/artkey/cjg-200202-0002_evaluation-of-genetic-diversity-in-19-glycine-max-l-merr-accessions-included-in-the-czech-national-collecti.phpsoybeanmolecular geneticsgenetic diversitydendogramrapd |
| spellingShingle | M. Baránek M. Kadlec J. Raddová M. Vachůn M. Pidra Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes Czech Journal of Genetics and Plant Breeding soybean molecular genetics genetic diversity dendogram rapd |
| title | Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes |
| title_full | Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes |
| title_fullStr | Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes |
| title_full_unstemmed | Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes |
| title_short | Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes |
| title_sort | evaluation of genetic diversity in 19 glycine max l merr accessions included in the czech national collection of soybean genotypes |
| topic | soybean molecular genetics genetic diversity dendogram rapd |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-200202-0002_evaluation-of-genetic-diversity-in-19-glycine-max-l-merr-accessions-included-in-the-czech-national-collecti.php |
| work_keys_str_mv | AT mbaranek evaluationofgeneticdiversityin19glycinemaxlmerraccessionsincludedintheczechnationalcollectionofsoybeangenotypes AT mkadlec evaluationofgeneticdiversityin19glycinemaxlmerraccessionsincludedintheczechnationalcollectionofsoybeangenotypes AT jraddova evaluationofgeneticdiversityin19glycinemaxlmerraccessionsincludedintheczechnationalcollectionofsoybeangenotypes AT mvachun evaluationofgeneticdiversityin19glycinemaxlmerraccessionsincludedintheczechnationalcollectionofsoybeangenotypes AT mpidra evaluationofgeneticdiversityin19glycinemaxlmerraccessionsincludedintheczechnationalcollectionofsoybeangenotypes |