Directing polymorph specific calcium carbonate formation with de novo protein templates
Abstract Biomolecules modulate inorganic crystallization to generate hierarchically structured biominerals, but the atomic structure of the organic-inorganic interfaces that regulate mineralization remain largely unknown. We hypothesized that heterogeneous nucleation of calcium carbonate could be ac...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-43608-1 |
| _version_ | 1827581499655847936 |
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| author | Fatima A. Davila-Hernandez Biao Jin Harley Pyles Shuai Zhang Zheming Wang Timothy F. Huddy Asim K. Bera Alex Kang Chun-Long Chen James J. De Yoreo David Baker |
| author_facet | Fatima A. Davila-Hernandez Biao Jin Harley Pyles Shuai Zhang Zheming Wang Timothy F. Huddy Asim K. Bera Alex Kang Chun-Long Chen James J. De Yoreo David Baker |
| author_sort | Fatima A. Davila-Hernandez |
| collection | DOAJ |
| description | Abstract Biomolecules modulate inorganic crystallization to generate hierarchically structured biominerals, but the atomic structure of the organic-inorganic interfaces that regulate mineralization remain largely unknown. We hypothesized that heterogeneous nucleation of calcium carbonate could be achieved by a structured flat molecular template that pre-organizes calcium ions on its surface. To test this hypothesis, we design helical repeat proteins (DHRs) displaying regularly spaced carboxylate arrays on their surfaces and find that both protein monomers and protein-Ca2+ supramolecular assemblies directly nucleate nano-calcite with non-natural {110} or {202} faces while vaterite, which forms first in the absence of the proteins, is bypassed. These protein-stabilized nanocrystals then assemble by oriented attachment into calcite mesocrystals. We find further that nanocrystal size and polymorph can be tuned by varying the length and surface chemistry of the designed protein templates. Thus, bio-mineralization can be programmed using de novo protein design, providing a route to next-generation hybrid materials. |
| first_indexed | 2024-03-08T22:37:22Z |
| format | Article |
| id | doaj.art-6cacd6f254a84ca6bc2400ddc3231925 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-08T22:37:22Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-6cacd6f254a84ca6bc2400ddc32319252023-12-17T12:22:22ZengNature PortfolioNature Communications2041-17232023-12-0114111110.1038/s41467-023-43608-1Directing polymorph specific calcium carbonate formation with de novo protein templatesFatima A. Davila-Hernandez0Biao Jin1Harley Pyles2Shuai Zhang3Zheming Wang4Timothy F. Huddy5Asim K. Bera6Alex Kang7Chun-Long Chen8James J. De Yoreo9David Baker10Department of Biochemistry, University of WashingtonPhysical Sciences Division, Pacific Northwest National LaboratoryDepartment of Biochemistry, University of WashingtonPhysical Sciences Division, Pacific Northwest National LaboratoryPhysical Sciences Division, Pacific Northwest National LaboratoryDepartment of Biochemistry, University of WashingtonDepartment of Biochemistry, University of WashingtonDepartment of Biochemistry, University of WashingtonPhysical Sciences Division, Pacific Northwest National LaboratoryPhysical Sciences Division, Pacific Northwest National LaboratoryDepartment of Biochemistry, University of WashingtonAbstract Biomolecules modulate inorganic crystallization to generate hierarchically structured biominerals, but the atomic structure of the organic-inorganic interfaces that regulate mineralization remain largely unknown. We hypothesized that heterogeneous nucleation of calcium carbonate could be achieved by a structured flat molecular template that pre-organizes calcium ions on its surface. To test this hypothesis, we design helical repeat proteins (DHRs) displaying regularly spaced carboxylate arrays on their surfaces and find that both protein monomers and protein-Ca2+ supramolecular assemblies directly nucleate nano-calcite with non-natural {110} or {202} faces while vaterite, which forms first in the absence of the proteins, is bypassed. These protein-stabilized nanocrystals then assemble by oriented attachment into calcite mesocrystals. We find further that nanocrystal size and polymorph can be tuned by varying the length and surface chemistry of the designed protein templates. Thus, bio-mineralization can be programmed using de novo protein design, providing a route to next-generation hybrid materials.https://doi.org/10.1038/s41467-023-43608-1 |
| spellingShingle | Fatima A. Davila-Hernandez Biao Jin Harley Pyles Shuai Zhang Zheming Wang Timothy F. Huddy Asim K. Bera Alex Kang Chun-Long Chen James J. De Yoreo David Baker Directing polymorph specific calcium carbonate formation with de novo protein templates Nature Communications |
| title | Directing polymorph specific calcium carbonate formation with de novo protein templates |
| title_full | Directing polymorph specific calcium carbonate formation with de novo protein templates |
| title_fullStr | Directing polymorph specific calcium carbonate formation with de novo protein templates |
| title_full_unstemmed | Directing polymorph specific calcium carbonate formation with de novo protein templates |
| title_short | Directing polymorph specific calcium carbonate formation with de novo protein templates |
| title_sort | directing polymorph specific calcium carbonate formation with de novo protein templates |
| url | https://doi.org/10.1038/s41467-023-43608-1 |
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