Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas
Abstract The objective of this study was to evaluate the efficiency of cryoprotective solution (PVS2) combined with phloroglucinol for the cryopreservation of seeds of two orchid species, Encyclia cordigera and Epidendrum ciliare. Seeds of Encyclia cordigera had 91.03% initial viability and 91.99% g...
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| Format: | Article |
| Language: | English |
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Instituto de Pesquisas Jardim Botânico do Rio de Janeiro
2021-12-01
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| Series: | Rodriguésia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100308&tlng=en |
| _version_ | 1818976740315234304 |
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| author | Suzana Targanski Sajovic Pereira Wagner Aparecido Vendrame Kathia Fernandes Lopes Pivetta José Carlos Sorgato Ricardo Tadeu de Faria |
| author_facet | Suzana Targanski Sajovic Pereira Wagner Aparecido Vendrame Kathia Fernandes Lopes Pivetta José Carlos Sorgato Ricardo Tadeu de Faria |
| author_sort | Suzana Targanski Sajovic Pereira |
| collection | DOAJ |
| description | Abstract The objective of this study was to evaluate the efficiency of cryoprotective solution (PVS2) combined with phloroglucinol for the cryopreservation of seeds of two orchid species, Encyclia cordigera and Epidendrum ciliare. Seeds of Encyclia cordigera had 91.03% initial viability and 91.99% germination. The treatment of the seeds with PVS2 at 0 °C with 1% phloroglucinol for 60 min returned 93.79% viability and 91.01% germination after recovery from LN, consequently resulting in faster development of protocorms. For Epidendrum ciliare, seed viability was 85.65% and germination was 85.90%. Seed exposure to the PVS2 at 0 °C with 1% phloroglucinol for 180 min showed viability of 39.23% and germination of 37.88%. Despite lower germination, 78.90% of the protocorms reached stage P3 of development, when evaluated 45 days after sowing, not significantly different from the control 1, and showed normal development. These results indicate that PVS2 cryoprotective solution is efficient when combined with phloroglucinol for the cryopreservation and successful recovery of seeds of Encyclia cordigera and Epidendrum ciliare. The present study also indicates that response to cryopreservation and success of recovery after cold storage is species-specific and requires adjustments in exposure time to PVS2 at 0 °C prior to immersion in LN. |
| first_indexed | 2024-12-20T16:16:39Z |
| format | Article |
| id | doaj.art-6caf49690eca4876a2894b0b988b11f2 |
| institution | Directory Open Access Journal |
| issn | 2175-7860 |
| language | English |
| last_indexed | 2024-12-20T16:16:39Z |
| publishDate | 2021-12-01 |
| publisher | Instituto de Pesquisas Jardim Botânico do Rio de Janeiro |
| record_format | Article |
| series | Rodriguésia |
| spelling | doaj.art-6caf49690eca4876a2894b0b988b11f22022-12-21T19:33:46ZengInstituto de Pesquisas Jardim Botânico do Rio de JaneiroRodriguésia2175-78602021-12-017210.1590/2175-7860202172123Efficiency of cryoprotectors for cryopreservation of two orchid species from AmericasSuzana Targanski Sajovic Pereirahttps://orcid.org/0000-0003-0424-489XWagner Aparecido Vendramehttps://orcid.org/0000-0001-6391-7623Kathia Fernandes Lopes Pivettahttps://orcid.org/0000-0001-9983-2402José Carlos Sorgatohttps://orcid.org/0000-0003-0001-5467Ricardo Tadeu de Fariahttps://orcid.org/0000-0002-7595-1965Abstract The objective of this study was to evaluate the efficiency of cryoprotective solution (PVS2) combined with phloroglucinol for the cryopreservation of seeds of two orchid species, Encyclia cordigera and Epidendrum ciliare. Seeds of Encyclia cordigera had 91.03% initial viability and 91.99% germination. The treatment of the seeds with PVS2 at 0 °C with 1% phloroglucinol for 60 min returned 93.79% viability and 91.01% germination after recovery from LN, consequently resulting in faster development of protocorms. For Epidendrum ciliare, seed viability was 85.65% and germination was 85.90%. Seed exposure to the PVS2 at 0 °C with 1% phloroglucinol for 180 min showed viability of 39.23% and germination of 37.88%. Despite lower germination, 78.90% of the protocorms reached stage P3 of development, when evaluated 45 days after sowing, not significantly different from the control 1, and showed normal development. These results indicate that PVS2 cryoprotective solution is efficient when combined with phloroglucinol for the cryopreservation and successful recovery of seeds of Encyclia cordigera and Epidendrum ciliare. The present study also indicates that response to cryopreservation and success of recovery after cold storage is species-specific and requires adjustments in exposure time to PVS2 at 0 °C prior to immersion in LN.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100308&tlng=enEncyclia cordigeraEpidendrum ciliaregermplasm conservationOrchidaceaePVS2 |
| spellingShingle | Suzana Targanski Sajovic Pereira Wagner Aparecido Vendrame Kathia Fernandes Lopes Pivetta José Carlos Sorgato Ricardo Tadeu de Faria Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas Rodriguésia Encyclia cordigera Epidendrum ciliare germplasm conservation Orchidaceae PVS2 |
| title | Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas |
| title_full | Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas |
| title_fullStr | Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas |
| title_full_unstemmed | Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas |
| title_short | Efficiency of cryoprotectors for cryopreservation of two orchid species from Americas |
| title_sort | efficiency of cryoprotectors for cryopreservation of two orchid species from americas |
| topic | Encyclia cordigera Epidendrum ciliare germplasm conservation Orchidaceae PVS2 |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S2175-78602021000100308&tlng=en |
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