Fluorescent reporter assays provide direct, accurate, quantitative measurements of MGMT status in human cells.
The DNA repair protein O6-methylguanine DNA methyltransferase (MGMT) strongly influences the effectiveness of cancer treatment with chemotherapeutic alkylating agents, and MGMT status in cancer cells could potentially contribute to tailored therapies for individual patients. However, the promoter me...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0208341 |
| _version_ | 1828213584495116288 |
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| author | Zachary D Nagel Andrew A Beharry Patrizia Mazzucato Gaspar J Kitange Jann N Sarkaria Eric T Kool Leona D Samson |
| author_facet | Zachary D Nagel Andrew A Beharry Patrizia Mazzucato Gaspar J Kitange Jann N Sarkaria Eric T Kool Leona D Samson |
| author_sort | Zachary D Nagel |
| collection | DOAJ |
| description | The DNA repair protein O6-methylguanine DNA methyltransferase (MGMT) strongly influences the effectiveness of cancer treatment with chemotherapeutic alkylating agents, and MGMT status in cancer cells could potentially contribute to tailored therapies for individual patients. However, the promoter methylation and immunohistochemical assays presently used for measuring MGMT in clinical samples are indirect, cumbersome and sometimes do not accurately report MGMT activity. Here we directly compare the accuracy of 6 analytical methods, including two fluorescent reporter assays, against the in vitro MGMT activity assay that is considered the gold standard for measuring MGMT DNA repair capacity. We discuss the relative advantages of each method. Our data indicate that two recently developed fluorescence-based assays measure MGMT activity accurately and efficiently, and could provide a functional dimension to clinical efforts to identify patients who are likely to benefit from alkylating chemotherapy. |
| first_indexed | 2024-04-12T14:52:34Z |
| format | Article |
| id | doaj.art-6cb244a315884924966a4e8becf14c02 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T14:52:34Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-6cb244a315884924966a4e8becf14c022022-12-22T03:28:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01142e020834110.1371/journal.pone.0208341Fluorescent reporter assays provide direct, accurate, quantitative measurements of MGMT status in human cells.Zachary D NagelAndrew A BeharryPatrizia MazzucatoGaspar J KitangeJann N SarkariaEric T KoolLeona D SamsonThe DNA repair protein O6-methylguanine DNA methyltransferase (MGMT) strongly influences the effectiveness of cancer treatment with chemotherapeutic alkylating agents, and MGMT status in cancer cells could potentially contribute to tailored therapies for individual patients. However, the promoter methylation and immunohistochemical assays presently used for measuring MGMT in clinical samples are indirect, cumbersome and sometimes do not accurately report MGMT activity. Here we directly compare the accuracy of 6 analytical methods, including two fluorescent reporter assays, against the in vitro MGMT activity assay that is considered the gold standard for measuring MGMT DNA repair capacity. We discuss the relative advantages of each method. Our data indicate that two recently developed fluorescence-based assays measure MGMT activity accurately and efficiently, and could provide a functional dimension to clinical efforts to identify patients who are likely to benefit from alkylating chemotherapy.https://doi.org/10.1371/journal.pone.0208341 |
| spellingShingle | Zachary D Nagel Andrew A Beharry Patrizia Mazzucato Gaspar J Kitange Jann N Sarkaria Eric T Kool Leona D Samson Fluorescent reporter assays provide direct, accurate, quantitative measurements of MGMT status in human cells. PLoS ONE |
| title | Fluorescent reporter assays provide direct, accurate, quantitative measurements of MGMT status in human cells. |
| title_full | Fluorescent reporter assays provide direct, accurate, quantitative measurements of MGMT status in human cells. |
| title_fullStr | Fluorescent reporter assays provide direct, accurate, quantitative measurements of MGMT status in human cells. |
| title_full_unstemmed | Fluorescent reporter assays provide direct, accurate, quantitative measurements of MGMT status in human cells. |
| title_short | Fluorescent reporter assays provide direct, accurate, quantitative measurements of MGMT status in human cells. |
| title_sort | fluorescent reporter assays provide direct accurate quantitative measurements of mgmt status in human cells |
| url | https://doi.org/10.1371/journal.pone.0208341 |
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