Butterfly Pea Flower (<i>Clitoria ternatea</i> Linn.) Extract Ameliorates Cardiovascular Dysfunction and Oxidative Stress in Nitric Oxide-Deficient Hypertensive Rats
In this study, we examine whether <i>Clitoria ternatea</i> Linn. (CT) can prevent Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced cardiac and vascular dysfunction in rats. Male Sprague Dawley rats were given L-NAME (40 mg/kg, drinking water) and orally administered with C...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/4/523 |
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| author | Putcharawipa Maneesai Metee Iampanichakul Nisita Chaihongsa Anuson Poasakate Prapassorn Potue Siwayu Rattanakanokchai Sarawoot Bunbupha Petcharat Chiangsaen Poungrat Pakdeechote |
| author_facet | Putcharawipa Maneesai Metee Iampanichakul Nisita Chaihongsa Anuson Poasakate Prapassorn Potue Siwayu Rattanakanokchai Sarawoot Bunbupha Petcharat Chiangsaen Poungrat Pakdeechote |
| author_sort | Putcharawipa Maneesai |
| collection | DOAJ |
| description | In this study, we examine whether <i>Clitoria ternatea</i> Linn. (CT) can prevent Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced cardiac and vascular dysfunction in rats. Male Sprague Dawley rats were given L-NAME (40 mg/kg, drinking water) and orally administered with CT extract (300 mg/kg/day) or lisinopril (2.5 mg/kg/day) for 5 weeks. The main phytochemical components of the CT extract were found to be flavonoids. The CT extract alleviated the high blood pressure in rats receiving L-NAME. Decreased vasorelaxation responses to acetylcholine and enhanced contractile responses to sympathetic nerve stimulation in aortic rings and mesenteric vascular beds of L-NAME treated rats were ameliorated by CT extract supplementation. Left ventricular hypertrophy and dysfunction were developed in L-NAME rats, which were partially prevented by CT extract treatment. The CT extract alleviated upregulated endothelial nitric oxide synthase expression, decreased plasma nitrate/nitrite levels, and increased oxidative stress in L-NAME rats. It suppressed high levels of serum angiotensin-converting enzyme activity, plasma angiotensin II, and cardiac angiotensin II type 1 receptor, NADPH oxidases 2, nuclear factor-kappa B, and tumor necrosis factor-alpha expression. The CT extract, therefore, partially prevented L-NAME-induced hypertension and cardiovascular alterations in rats. These effects might be related to a reduction in the oxidative stress and renin–angiotensin system activation due to L-NAME in rats. |
| first_indexed | 2024-03-10T12:51:17Z |
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| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T12:51:17Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-6cb4e8709f974cbfb15339a8a2f81ee22023-11-21T13:03:25ZengMDPI AGAntioxidants2076-39212021-03-0110452310.3390/antiox10040523Butterfly Pea Flower (<i>Clitoria ternatea</i> Linn.) Extract Ameliorates Cardiovascular Dysfunction and Oxidative Stress in Nitric Oxide-Deficient Hypertensive RatsPutcharawipa Maneesai0Metee Iampanichakul1Nisita Chaihongsa2Anuson Poasakate3Prapassorn Potue4Siwayu Rattanakanokchai5Sarawoot Bunbupha6Petcharat Chiangsaen7Poungrat Pakdeechote8Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandFaculty of Veterinary Medicine, Khon Kaen University, Khon Kaen 40002, ThailandFaculty of Medicine, Mahasarakham University, Maha Sarakham 44000, ThailandFaculty of Medicine, Bangkokthonburi University, Bangkok 10170, ThailandDepartment of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, ThailandIn this study, we examine whether <i>Clitoria ternatea</i> Linn. (CT) can prevent Nω-nitro-L-arginine methyl ester hydrochloride (L-NAME)-induced cardiac and vascular dysfunction in rats. Male Sprague Dawley rats were given L-NAME (40 mg/kg, drinking water) and orally administered with CT extract (300 mg/kg/day) or lisinopril (2.5 mg/kg/day) for 5 weeks. The main phytochemical components of the CT extract were found to be flavonoids. The CT extract alleviated the high blood pressure in rats receiving L-NAME. Decreased vasorelaxation responses to acetylcholine and enhanced contractile responses to sympathetic nerve stimulation in aortic rings and mesenteric vascular beds of L-NAME treated rats were ameliorated by CT extract supplementation. Left ventricular hypertrophy and dysfunction were developed in L-NAME rats, which were partially prevented by CT extract treatment. The CT extract alleviated upregulated endothelial nitric oxide synthase expression, decreased plasma nitrate/nitrite levels, and increased oxidative stress in L-NAME rats. It suppressed high levels of serum angiotensin-converting enzyme activity, plasma angiotensin II, and cardiac angiotensin II type 1 receptor, NADPH oxidases 2, nuclear factor-kappa B, and tumor necrosis factor-alpha expression. The CT extract, therefore, partially prevented L-NAME-induced hypertension and cardiovascular alterations in rats. These effects might be related to a reduction in the oxidative stress and renin–angiotensin system activation due to L-NAME in rats.https://www.mdpi.com/2076-3921/10/4/523<i>Clitoria ternatea</i> Linn.left ventricular and vascular dysfunctionoxidative stressrenin–angiotensin systeminflammation |
| spellingShingle | Putcharawipa Maneesai Metee Iampanichakul Nisita Chaihongsa Anuson Poasakate Prapassorn Potue Siwayu Rattanakanokchai Sarawoot Bunbupha Petcharat Chiangsaen Poungrat Pakdeechote Butterfly Pea Flower (<i>Clitoria ternatea</i> Linn.) Extract Ameliorates Cardiovascular Dysfunction and Oxidative Stress in Nitric Oxide-Deficient Hypertensive Rats Antioxidants <i>Clitoria ternatea</i> Linn. left ventricular and vascular dysfunction oxidative stress renin–angiotensin system inflammation |
| title | Butterfly Pea Flower (<i>Clitoria ternatea</i> Linn.) Extract Ameliorates Cardiovascular Dysfunction and Oxidative Stress in Nitric Oxide-Deficient Hypertensive Rats |
| title_full | Butterfly Pea Flower (<i>Clitoria ternatea</i> Linn.) Extract Ameliorates Cardiovascular Dysfunction and Oxidative Stress in Nitric Oxide-Deficient Hypertensive Rats |
| title_fullStr | Butterfly Pea Flower (<i>Clitoria ternatea</i> Linn.) Extract Ameliorates Cardiovascular Dysfunction and Oxidative Stress in Nitric Oxide-Deficient Hypertensive Rats |
| title_full_unstemmed | Butterfly Pea Flower (<i>Clitoria ternatea</i> Linn.) Extract Ameliorates Cardiovascular Dysfunction and Oxidative Stress in Nitric Oxide-Deficient Hypertensive Rats |
| title_short | Butterfly Pea Flower (<i>Clitoria ternatea</i> Linn.) Extract Ameliorates Cardiovascular Dysfunction and Oxidative Stress in Nitric Oxide-Deficient Hypertensive Rats |
| title_sort | butterfly pea flower i clitoria ternatea i linn extract ameliorates cardiovascular dysfunction and oxidative stress in nitric oxide deficient hypertensive rats |
| topic | <i>Clitoria ternatea</i> Linn. left ventricular and vascular dysfunction oxidative stress renin–angiotensin system inflammation |
| url | https://www.mdpi.com/2076-3921/10/4/523 |
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