Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation
After sperm-oocyte fusion, intracytoplasmic rises of calcium (Ca) induce the release of zinc (Zn) out of the oocyte (Zn sparks). Both phenomena are known to play an essential role in the oocyte activation process. Our work aimed to explore different protocols for activating bovine and porcine oocyte...
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MDPI AG
2022-12-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/24/3560 |
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| author | Juan P. Cabeza Juan Cámera Olinda Briski Minerva Yauri Felipe Daniel F. Salamone Andrés Gambini |
| author_facet | Juan P. Cabeza Juan Cámera Olinda Briski Minerva Yauri Felipe Daniel F. Salamone Andrés Gambini |
| author_sort | Juan P. Cabeza |
| collection | DOAJ |
| description | After sperm-oocyte fusion, intracytoplasmic rises of calcium (Ca) induce the release of zinc (Zn) out of the oocyte (Zn sparks). Both phenomena are known to play an essential role in the oocyte activation process. Our work aimed to explore different protocols for activating bovine and porcine oocytes using the novel zinc chelator 1,10-phenanthroline (PHEN) and to compare developmental rates and quality to bovine IVF and parthenogenetic ionomycin-induced embryos in both species. Different incubation conditions for the zinc chelator were tested, including its combination with ionomycin. Embryo quality was assessed by immunofluorescence of SOX2, SOX17, OCT4, and CDX2 and total cell number at the blastocyst stage. Even though blastocyst development was achieved using a zinc chelator in bovine, bypassing calcium oscillations, developmental rates, and blastocyst quality were compromised compared to embryos generated with sperm-induced or ionomycin calcium rise. On the contrary, zinc chelation is sufficient to trigger oocyte activation in porcine. Additionally, we determined the optimal exposure to PHEN for this species. Zinc chelation and artificial induction of calcium rise combined did not improve developmental competence. Our results contribute to understanding the role of zinc during oocyte activation and preimplantation embryo development across different mammalian species. |
| first_indexed | 2024-03-09T17:24:57Z |
| format | Article |
| id | doaj.art-6cb5e83800ec44a1b31d24bcb305aff0 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T17:24:57Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-6cb5e83800ec44a1b31d24bcb305aff02023-11-24T12:51:53ZengMDPI AGAnimals2076-26152022-12-011224356010.3390/ani12243560Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc ChelationJuan P. Cabeza0Juan Cámera1Olinda Briski2Minerva Yauri Felipe3Daniel F. Salamone4Andrés Gambini5Facultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Buenos Aires C1417DSE, ArgentinaFacultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Buenos Aires C1417DSE, ArgentinaFacultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Buenos Aires C1417DSE, ArgentinaFacultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Buenos Aires C1417DSE, ArgentinaFacultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Buenos Aires C1417DSE, ArgentinaFacultad de Agronomía, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Buenos Aires C1417DSE, ArgentinaAfter sperm-oocyte fusion, intracytoplasmic rises of calcium (Ca) induce the release of zinc (Zn) out of the oocyte (Zn sparks). Both phenomena are known to play an essential role in the oocyte activation process. Our work aimed to explore different protocols for activating bovine and porcine oocytes using the novel zinc chelator 1,10-phenanthroline (PHEN) and to compare developmental rates and quality to bovine IVF and parthenogenetic ionomycin-induced embryos in both species. Different incubation conditions for the zinc chelator were tested, including its combination with ionomycin. Embryo quality was assessed by immunofluorescence of SOX2, SOX17, OCT4, and CDX2 and total cell number at the blastocyst stage. Even though blastocyst development was achieved using a zinc chelator in bovine, bypassing calcium oscillations, developmental rates, and blastocyst quality were compromised compared to embryos generated with sperm-induced or ionomycin calcium rise. On the contrary, zinc chelation is sufficient to trigger oocyte activation in porcine. Additionally, we determined the optimal exposure to PHEN for this species. Zinc chelation and artificial induction of calcium rise combined did not improve developmental competence. Our results contribute to understanding the role of zinc during oocyte activation and preimplantation embryo development across different mammalian species.https://www.mdpi.com/2076-2615/12/24/3560assisted oocyte activationembryo developmentoocyte to embryo transitionzinc chelatorcalcium oscillation |
| spellingShingle | Juan P. Cabeza Juan Cámera Olinda Briski Minerva Yauri Felipe Daniel F. Salamone Andrés Gambini Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation Animals assisted oocyte activation embryo development oocyte to embryo transition zinc chelator calcium oscillation |
| title | Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation |
| title_full | Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation |
| title_fullStr | Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation |
| title_full_unstemmed | Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation |
| title_short | Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation |
| title_sort | preimplantation developmental competence of bovine and porcine oocytes activated by zinc chelation |
| topic | assisted oocyte activation embryo development oocyte to embryo transition zinc chelator calcium oscillation |
| url | https://www.mdpi.com/2076-2615/12/24/3560 |
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