Assessing Cellular Uptake of Exogenous Coenzyme Q<sub>10</sub> into Human Skin Cells by X-ray Fluorescence Imaging
X-ray fluorescence (XRF) imaging is a highly sensitive non-invasive imaging method for detection of small element quantities in objects, from human-sized scales down to single-cell organelles, using various X-ray beam sizes. Our aim was to investigate the cellular uptake and distribution of Q<sub...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | Antioxidants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3921/11/8/1532 |
| _version_ | 1797411426562736128 |
|---|---|
| author | Theresa Staufer Mirja L. Schulze Oliver Schmutzler Christian Körnig Vivienne Welge Thorsten Burkhardt Jens-Peter Vietzke Alexandra Vogelsang Julia M. Weise Thomas Blatt Oliver Dabrowski Gerald Falkenberg Dennis Brückner Carlos Sanchez-Cano Florian Grüner |
| author_facet | Theresa Staufer Mirja L. Schulze Oliver Schmutzler Christian Körnig Vivienne Welge Thorsten Burkhardt Jens-Peter Vietzke Alexandra Vogelsang Julia M. Weise Thomas Blatt Oliver Dabrowski Gerald Falkenberg Dennis Brückner Carlos Sanchez-Cano Florian Grüner |
| author_sort | Theresa Staufer |
| collection | DOAJ |
| description | X-ray fluorescence (XRF) imaging is a highly sensitive non-invasive imaging method for detection of small element quantities in objects, from human-sized scales down to single-cell organelles, using various X-ray beam sizes. Our aim was to investigate the cellular uptake and distribution of Q<sub>10</sub>, a highly conserved coenzyme with antioxidant and bioenergetic properties. Q<sub>10</sub> was labeled with iodine (I<sub>2</sub>-Q<sub>10</sub>) and individual primary human skin cells were scanned with nano-focused beams. Distribution of I<sub>2</sub>-Q<sub>10</sub> molecules taken up inside the screened individual skin cells was measured, with a clear correlation between individual Q<sub>10</sub> uptake and cell size. Experiments revealed that labeling Q<sub>10</sub> with iodine causes no artificial side effects as a result of the labeling procedure itself, and thus is a perfect means of investigating bioavailability and distribution of Q<sub>10</sub> in cells. In summary, individual cellular Q<sub>10</sub> uptake was demonstrated by XRF, opening the path towards Q<sub>10</sub> multi-scale tracking for biodistribution studies. |
| first_indexed | 2024-03-09T04:45:58Z |
| format | Article |
| id | doaj.art-6cbcf03f3be142a9a74b22a27e9ab049 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T04:45:58Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-6cbcf03f3be142a9a74b22a27e9ab0492023-12-03T13:15:51ZengMDPI AGAntioxidants2076-39212022-08-01118153210.3390/antiox11081532Assessing Cellular Uptake of Exogenous Coenzyme Q<sub>10</sub> into Human Skin Cells by X-ray Fluorescence ImagingTheresa Staufer0Mirja L. Schulze1Oliver Schmutzler2Christian Körnig3Vivienne Welge4Thorsten Burkhardt5Jens-Peter Vietzke6Alexandra Vogelsang7Julia M. Weise8Thomas Blatt9Oliver Dabrowski10Gerald Falkenberg11Dennis Brückner12Carlos Sanchez-Cano13Florian Grüner14Universität Hamburg and Center for Free-Electron Laser Science (CFEL), Institute for Experimental Physics, Faculty for Mathematics, Informatics and Natural Sciences, Luruper Chaussee 149, 22761 Hamburg, GermanyResearch and Development, Beiersdorf AG, Unnastrasse 48, 20245 Hamburg, GermanyUniversität Hamburg and Center for Free-Electron Laser Science (CFEL), Institute for Experimental Physics, Faculty for Mathematics, Informatics and Natural Sciences, Luruper Chaussee 149, 22761 Hamburg, GermanyUniversität Hamburg and Center for Free-Electron Laser Science (CFEL), Institute for Experimental Physics, Faculty for Mathematics, Informatics and Natural Sciences, Luruper Chaussee 149, 22761 Hamburg, GermanyResearch and Development, Beiersdorf AG, Unnastrasse 48, 20245 Hamburg, GermanyResearch and Development, Beiersdorf AG, Unnastrasse 48, 20245 Hamburg, GermanyResearch and Development, Beiersdorf AG, Unnastrasse 48, 20245 Hamburg, GermanyResearch and Development, Beiersdorf AG, Unnastrasse 48, 20245 Hamburg, GermanyResearch and Development, Beiersdorf AG, Unnastrasse 48, 20245 Hamburg, GermanyResearch and Development, Beiersdorf AG, Unnastrasse 48, 20245 Hamburg, GermanyFraunhofer Institute for Applied Polymer Research (IAP), Center for Applied Nanotechnology (CAN), Grindelallee 117, 20146 Hamburg, GermanyDeutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, GermanyDeutsches Elektronen-Synchrotron DESY, Notkestrasse 85, 22607 Hamburg, GermanyDIPC, Paseo Manuel de Lardizabal 4, 20018 Donostia-San Sebastian, SpainUniversität Hamburg and Center for Free-Electron Laser Science (CFEL), Institute for Experimental Physics, Faculty for Mathematics, Informatics and Natural Sciences, Luruper Chaussee 149, 22761 Hamburg, GermanyX-ray fluorescence (XRF) imaging is a highly sensitive non-invasive imaging method for detection of small element quantities in objects, from human-sized scales down to single-cell organelles, using various X-ray beam sizes. Our aim was to investigate the cellular uptake and distribution of Q<sub>10</sub>, a highly conserved coenzyme with antioxidant and bioenergetic properties. Q<sub>10</sub> was labeled with iodine (I<sub>2</sub>-Q<sub>10</sub>) and individual primary human skin cells were scanned with nano-focused beams. Distribution of I<sub>2</sub>-Q<sub>10</sub> molecules taken up inside the screened individual skin cells was measured, with a clear correlation between individual Q<sub>10</sub> uptake and cell size. Experiments revealed that labeling Q<sub>10</sub> with iodine causes no artificial side effects as a result of the labeling procedure itself, and thus is a perfect means of investigating bioavailability and distribution of Q<sub>10</sub> in cells. In summary, individual cellular Q<sub>10</sub> uptake was demonstrated by XRF, opening the path towards Q<sub>10</sub> multi-scale tracking for biodistribution studies.https://www.mdpi.com/2076-3921/11/8/1532X-ray fluorescence (XRF) imagingQ<sub>10</sub>uptakesingle skin cells |
| spellingShingle | Theresa Staufer Mirja L. Schulze Oliver Schmutzler Christian Körnig Vivienne Welge Thorsten Burkhardt Jens-Peter Vietzke Alexandra Vogelsang Julia M. Weise Thomas Blatt Oliver Dabrowski Gerald Falkenberg Dennis Brückner Carlos Sanchez-Cano Florian Grüner Assessing Cellular Uptake of Exogenous Coenzyme Q<sub>10</sub> into Human Skin Cells by X-ray Fluorescence Imaging Antioxidants X-ray fluorescence (XRF) imaging Q<sub>10</sub> uptake single skin cells |
| title | Assessing Cellular Uptake of Exogenous Coenzyme Q<sub>10</sub> into Human Skin Cells by X-ray Fluorescence Imaging |
| title_full | Assessing Cellular Uptake of Exogenous Coenzyme Q<sub>10</sub> into Human Skin Cells by X-ray Fluorescence Imaging |
| title_fullStr | Assessing Cellular Uptake of Exogenous Coenzyme Q<sub>10</sub> into Human Skin Cells by X-ray Fluorescence Imaging |
| title_full_unstemmed | Assessing Cellular Uptake of Exogenous Coenzyme Q<sub>10</sub> into Human Skin Cells by X-ray Fluorescence Imaging |
| title_short | Assessing Cellular Uptake of Exogenous Coenzyme Q<sub>10</sub> into Human Skin Cells by X-ray Fluorescence Imaging |
| title_sort | assessing cellular uptake of exogenous coenzyme q sub 10 sub into human skin cells by x ray fluorescence imaging |
| topic | X-ray fluorescence (XRF) imaging Q<sub>10</sub> uptake single skin cells |
| url | https://www.mdpi.com/2076-3921/11/8/1532 |
| work_keys_str_mv | AT theresastaufer assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT mirjalschulze assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT oliverschmutzler assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT christiankornig assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT viviennewelge assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT thorstenburkhardt assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT jenspetervietzke assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT alexandravogelsang assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT juliamweise assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT thomasblatt assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT oliverdabrowski assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT geraldfalkenberg assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT dennisbruckner assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT carlossanchezcano assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging AT floriangruner assessingcellularuptakeofexogenouscoenzymeqsub10subintohumanskincellsbyxrayfluorescenceimaging |