LINC00106/RPS19BP1/p53 axis promotes the proliferation and migration of human prostate cancer cells
Background Prostate cancer (PCa) is among the most prevalent cancers in males with high biochemical recurrence risk. LINC00106 contributes to the carcinogenesis of Hepatocellular carcinoma (HCC). However, it is unclear how it affects PCa advancement. Here, we studied LINC00106’s effects on PCa cells...
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PeerJ Inc.
2023-05-01
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| Online Access: | https://peerj.com/articles/15232.pdf |
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| author | Lingxiang Lu Zhen Tian Jicheng Lu Minjun Jiang Jianchun Chen Shuai Guo Yuhua Huang |
| author_facet | Lingxiang Lu Zhen Tian Jicheng Lu Minjun Jiang Jianchun Chen Shuai Guo Yuhua Huang |
| author_sort | Lingxiang Lu |
| collection | DOAJ |
| description | Background Prostate cancer (PCa) is among the most prevalent cancers in males with high biochemical recurrence risk. LINC00106 contributes to the carcinogenesis of Hepatocellular carcinoma (HCC). However, it is unclear how it affects PCa advancement. Here, we studied LINC00106’s effects on PCa cells’ ability to proliferate, invade, and metastasize. Methods The data of LINC00106 from The Cancer Genome Atlas (TCGA) in human PCa tissues were analyzed using TANRIC and survival analysis. In order to determine the expression levels of genes and proteins, we also performed reverse transcription-quantitative PCR and western blot analysis. The migration, invasion, colony formation, and proliferation (CCK-8) of PCa cells with LINC00106 knockdown were investigated. The impact of LINC00106 on cell proliferation and invasion was also analyzed in mice. LncRNA prediction software catRAPID omics v2.1 (catRAPID omics v2.0 (tartaglialab.com)) was used to predict proteins that might interact with LINC00106. The interactions were verified via RNA immunoprecipitation and RNA pull-down assays and finally, the interaction between LINC00106 and its target protein and the p53 signaling pathway was studied using a dual-luciferase reporter assay. Results In PCa, LINC00106 was over-expressed in comparison to normal tissues, and it was linked to an unfavorableprognosis. In vitro and in vivo analyses showed that downregulating LINC00106 decreased PCa cells’ability to proliferate and migrate. A common regulatory axis generated by LINC00106 and RPS19BP1 prevents p53 activity. Conclusion Our experimental data indicate that LINC00106 functions as an oncogene in the onset of PCa, and the LINC00106/RPS19BP1/P53 axis canserve as a novel therapeutic target for PCa treatment. |
| first_indexed | 2024-03-09T04:29:48Z |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T04:29:48Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-6cc04fd5f1f8450cb982c9c7bd5460332023-12-03T13:36:33ZengPeerJ Inc.PeerJ2167-83592023-05-0111e1523210.7717/peerj.15232LINC00106/RPS19BP1/p53 axis promotes the proliferation and migration of human prostate cancer cellsLingxiang Lu0Zhen Tian1Jicheng Lu2Minjun Jiang3Jianchun Chen4Shuai Guo5Yuhua Huang6Department of Urinary Surgery, Suzhou Ninth People’s Hospital, Soochow University, Suzhou, Jiangsu, ChinaDepartment of Urinary Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, ChinaOncology Department, Suzhou Ninth People’s Hospital, Soochow University, Suzhou, Jiangsu, ChinaDepartment of Urinary Surgery, Suzhou Ninth People’s Hospital, Soochow University, Suzhou, Jiangsu, ChinaDepartment of Urinary Surgery, Suzhou Ninth People’s Hospital, Soochow University, Suzhou, Jiangsu, ChinaDepartment of Urinary Surgery, Suzhou Ninth People’s Hospital, Soochow University, Suzhou, Jiangsu, ChinaDepartment of Urinary Surgery, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu, ChinaBackground Prostate cancer (PCa) is among the most prevalent cancers in males with high biochemical recurrence risk. LINC00106 contributes to the carcinogenesis of Hepatocellular carcinoma (HCC). However, it is unclear how it affects PCa advancement. Here, we studied LINC00106’s effects on PCa cells’ ability to proliferate, invade, and metastasize. Methods The data of LINC00106 from The Cancer Genome Atlas (TCGA) in human PCa tissues were analyzed using TANRIC and survival analysis. In order to determine the expression levels of genes and proteins, we also performed reverse transcription-quantitative PCR and western blot analysis. The migration, invasion, colony formation, and proliferation (CCK-8) of PCa cells with LINC00106 knockdown were investigated. The impact of LINC00106 on cell proliferation and invasion was also analyzed in mice. LncRNA prediction software catRAPID omics v2.1 (catRAPID omics v2.0 (tartaglialab.com)) was used to predict proteins that might interact with LINC00106. The interactions were verified via RNA immunoprecipitation and RNA pull-down assays and finally, the interaction between LINC00106 and its target protein and the p53 signaling pathway was studied using a dual-luciferase reporter assay. Results In PCa, LINC00106 was over-expressed in comparison to normal tissues, and it was linked to an unfavorableprognosis. In vitro and in vivo analyses showed that downregulating LINC00106 decreased PCa cells’ability to proliferate and migrate. A common regulatory axis generated by LINC00106 and RPS19BP1 prevents p53 activity. Conclusion Our experimental data indicate that LINC00106 functions as an oncogene in the onset of PCa, and the LINC00106/RPS19BP1/P53 axis canserve as a novel therapeutic target for PCa treatment.https://peerj.com/articles/15232.pdfLINC00106Prostate cancerRPS19BP1p53 |
| spellingShingle | Lingxiang Lu Zhen Tian Jicheng Lu Minjun Jiang Jianchun Chen Shuai Guo Yuhua Huang LINC00106/RPS19BP1/p53 axis promotes the proliferation and migration of human prostate cancer cells PeerJ LINC00106 Prostate cancer RPS19BP1 p53 |
| title | LINC00106/RPS19BP1/p53 axis promotes the proliferation and migration of human prostate cancer cells |
| title_full | LINC00106/RPS19BP1/p53 axis promotes the proliferation and migration of human prostate cancer cells |
| title_fullStr | LINC00106/RPS19BP1/p53 axis promotes the proliferation and migration of human prostate cancer cells |
| title_full_unstemmed | LINC00106/RPS19BP1/p53 axis promotes the proliferation and migration of human prostate cancer cells |
| title_short | LINC00106/RPS19BP1/p53 axis promotes the proliferation and migration of human prostate cancer cells |
| title_sort | linc00106 rps19bp1 p53 axis promotes the proliferation and migration of human prostate cancer cells |
| topic | LINC00106 Prostate cancer RPS19BP1 p53 |
| url | https://peerj.com/articles/15232.pdf |
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