CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering
The Chinese hamster ovary (CHO) cell line is a well-established platform for the production of biopharmaceuticals due to its ability to express complex therapeutic proteins with human-like glycopatterns in high amounts. The advent of CRISPR technology has opened up new avenues for the engineering of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/9/8144 |
| _version_ | 1797602445309771776 |
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| author | Katja Glinšek Krištof Bozovičar Tomaž Bratkovič |
| author_facet | Katja Glinšek Krištof Bozovičar Tomaž Bratkovič |
| author_sort | Katja Glinšek |
| collection | DOAJ |
| description | The Chinese hamster ovary (CHO) cell line is a well-established platform for the production of biopharmaceuticals due to its ability to express complex therapeutic proteins with human-like glycopatterns in high amounts. The advent of CRISPR technology has opened up new avenues for the engineering of CHO cell lines for improved protein production and enhanced product quality. This review summarizes recent advances in the application of CRISPR technology for CHO cell line engineering with a particular focus on glycosylation modulation, productivity enhancement, tackling adventitious agents, elimination of problematic host cell proteins, development of antibiotic-free selection systems, site-specific transgene integration, and CRISPR-mediated gene activation and repression. The review highlights the potential of CRISPR technology in CHO cell line genome editing and epigenetic engineering for the more efficient and cost-effective development of biopharmaceuticals while ensuring the safety and quality of the final product. |
| first_indexed | 2024-03-11T04:16:12Z |
| format | Article |
| id | doaj.art-6cc4637829b94771a52c4a75d0c7cab5 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T04:16:12Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-6cc4637829b94771a52c4a75d0c7cab52023-11-17T23:05:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-01249814410.3390/ijms24098144CRISPR Technologies in Chinese Hamster Ovary Cell Line EngineeringKatja Glinšek0Krištof Bozovičar1Tomaž Bratkovič2Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000 Ljubljana, SloveniaFaculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000 Ljubljana, SloveniaFaculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000 Ljubljana, SloveniaThe Chinese hamster ovary (CHO) cell line is a well-established platform for the production of biopharmaceuticals due to its ability to express complex therapeutic proteins with human-like glycopatterns in high amounts. The advent of CRISPR technology has opened up new avenues for the engineering of CHO cell lines for improved protein production and enhanced product quality. This review summarizes recent advances in the application of CRISPR technology for CHO cell line engineering with a particular focus on glycosylation modulation, productivity enhancement, tackling adventitious agents, elimination of problematic host cell proteins, development of antibiotic-free selection systems, site-specific transgene integration, and CRISPR-mediated gene activation and repression. The review highlights the potential of CRISPR technology in CHO cell line genome editing and epigenetic engineering for the more efficient and cost-effective development of biopharmaceuticals while ensuring the safety and quality of the final product.https://www.mdpi.com/1422-0067/24/9/8144Chinese hamster ovary cellscell line developmentCRISPR-Casgenome editingglycoengineeringaugmenting productivity |
| spellingShingle | Katja Glinšek Krištof Bozovičar Tomaž Bratkovič CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering International Journal of Molecular Sciences Chinese hamster ovary cells cell line development CRISPR-Cas genome editing glycoengineering augmenting productivity |
| title | CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering |
| title_full | CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering |
| title_fullStr | CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering |
| title_full_unstemmed | CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering |
| title_short | CRISPR Technologies in Chinese Hamster Ovary Cell Line Engineering |
| title_sort | crispr technologies in chinese hamster ovary cell line engineering |
| topic | Chinese hamster ovary cells cell line development CRISPR-Cas genome editing glycoengineering augmenting productivity |
| url | https://www.mdpi.com/1422-0067/24/9/8144 |
| work_keys_str_mv | AT katjaglinsek crisprtechnologiesinchinesehamsterovarycelllineengineering AT kristofbozovicar crisprtechnologiesinchinesehamsterovarycelllineengineering AT tomazbratkovic crisprtechnologiesinchinesehamsterovarycelllineengineering |