Emerging Applications of Electrochemical Impedance Spectroscopy in Tear Film Analysis
Human tear film, with a flow rate of 1–3 µL/min, is a rich bodily fluid that transmits a variety of metabolites and hormones containing proteins, lipids and electrolytes that provide clues about ocular and systemic diseases. Analysis of disease biomarkers such as proteins, mRNA, enzymes and cytokine...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/10/827 |
| _version_ | 1797474782677041152 |
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| author | Berin Ozdalgic Munire Gul Zihni Onur Uygun Nazente Atçeken Savas Tasoglu |
| author_facet | Berin Ozdalgic Munire Gul Zihni Onur Uygun Nazente Atçeken Savas Tasoglu |
| author_sort | Berin Ozdalgic |
| collection | DOAJ |
| description | Human tear film, with a flow rate of 1–3 µL/min, is a rich bodily fluid that transmits a variety of metabolites and hormones containing proteins, lipids and electrolytes that provide clues about ocular and systemic diseases. Analysis of disease biomarkers such as proteins, mRNA, enzymes and cytokines in the tear film, collected by noninvasive methods, can provide significant results for sustaining a predictive, preventive and personalized medicine regarding various diseases such as glaucoma, diabetic retinopathy, keratoconus, dry eye, cancer, Alzheimer’s disease, Parkinson’s disease and COVID-19. Electrochemical impedance spectroscopy (EIS) offers a powerful technique for analyzing these biomarkers. EIS detects electrical equivalent circuit parameters related to biorecognition of receptor–analyte interactions on the electrode surface. This method is advantageous as it performs a label-free detection and allows the detection of non-electroactive compounds that cannot be detected by direct electron transfer, such as hormones and some proteins. Here, we review the opportunities regarding the integration of EIS into tear fluid sampling approaches. |
| first_indexed | 2024-03-09T20:35:58Z |
| format | Article |
| id | doaj.art-6cc925766db34a8b8e3c24340d6446ad |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-09T20:35:58Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-6cc925766db34a8b8e3c24340d6446ad2023-11-23T23:11:18ZengMDPI AGBiosensors2079-63742022-10-01121082710.3390/bios12100827Emerging Applications of Electrochemical Impedance Spectroscopy in Tear Film AnalysisBerin Ozdalgic0Munire Gul1Zihni Onur Uygun2Nazente Atçeken3Savas Tasoglu4Department of Mechanical Engineering, Engineering Faculty, Koç University, Istanbul 34450, TürkiyeKoç University Translational Medicine Research Center (KUTTAM), Koç University, Istanbul 34450, TürkiyeDepartment of Mechanical Engineering, Engineering Faculty, Koç University, Istanbul 34450, TürkiyeKoç University Translational Medicine Research Center (KUTTAM), Koç University, Istanbul 34450, TürkiyeDepartment of Mechanical Engineering, Engineering Faculty, Koç University, Istanbul 34450, TürkiyeHuman tear film, with a flow rate of 1–3 µL/min, is a rich bodily fluid that transmits a variety of metabolites and hormones containing proteins, lipids and electrolytes that provide clues about ocular and systemic diseases. Analysis of disease biomarkers such as proteins, mRNA, enzymes and cytokines in the tear film, collected by noninvasive methods, can provide significant results for sustaining a predictive, preventive and personalized medicine regarding various diseases such as glaucoma, diabetic retinopathy, keratoconus, dry eye, cancer, Alzheimer’s disease, Parkinson’s disease and COVID-19. Electrochemical impedance spectroscopy (EIS) offers a powerful technique for analyzing these biomarkers. EIS detects electrical equivalent circuit parameters related to biorecognition of receptor–analyte interactions on the electrode surface. This method is advantageous as it performs a label-free detection and allows the detection of non-electroactive compounds that cannot be detected by direct electron transfer, such as hormones and some proteins. Here, we review the opportunities regarding the integration of EIS into tear fluid sampling approaches.https://www.mdpi.com/2079-6374/12/10/827biosensingocular diagnosissystemic disease diagnosistear fluidelectrochemical impedance spectroscopy |
| spellingShingle | Berin Ozdalgic Munire Gul Zihni Onur Uygun Nazente Atçeken Savas Tasoglu Emerging Applications of Electrochemical Impedance Spectroscopy in Tear Film Analysis Biosensors biosensing ocular diagnosis systemic disease diagnosis tear fluid electrochemical impedance spectroscopy |
| title | Emerging Applications of Electrochemical Impedance Spectroscopy in Tear Film Analysis |
| title_full | Emerging Applications of Electrochemical Impedance Spectroscopy in Tear Film Analysis |
| title_fullStr | Emerging Applications of Electrochemical Impedance Spectroscopy in Tear Film Analysis |
| title_full_unstemmed | Emerging Applications of Electrochemical Impedance Spectroscopy in Tear Film Analysis |
| title_short | Emerging Applications of Electrochemical Impedance Spectroscopy in Tear Film Analysis |
| title_sort | emerging applications of electrochemical impedance spectroscopy in tear film analysis |
| topic | biosensing ocular diagnosis systemic disease diagnosis tear fluid electrochemical impedance spectroscopy |
| url | https://www.mdpi.com/2079-6374/12/10/827 |
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