Sequence and biochemical analysis of Arabidopsis SP1 protein, a regulator of organelle biogenesis
Peroxisomes, chloroplasts, and mitochondria are essential eukaryotic organelles that host a suite of metabolic processes crucial to energy metabolism and development. Regulatory mechanisms of the dynamics and biogenesis of these important organelles have begun to be discovered in plants. We recently...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-07-01
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| Series: | Communicative & Integrative Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/19420889.2017.1338991 |
| _version_ | 1818663922744426496 |
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| author | Ronghui Pan Jianping Hu |
| author_facet | Ronghui Pan Jianping Hu |
| author_sort | Ronghui Pan |
| collection | DOAJ |
| description | Peroxisomes, chloroplasts, and mitochondria are essential eukaryotic organelles that host a suite of metabolic processes crucial to energy metabolism and development. Regulatory mechanisms of the dynamics and biogenesis of these important organelles have begun to be discovered in plants. We recently showed that, aside from its previously reported role in targeting chloroplast protein import proteins, the Arabidopsis ubiquitin E3 ligase SP1 (suppressor of ppi1 locus1) negatively regulates peroxisome matrix protein import by promoting the ubiquitination and destabilization of PEX13 and possibly PEX14 and other components of the peroxisome protein import apparatus. Here, we compared protein sequence and domain structure of SP1-like proteins in Arabidopsis and their human homolog, Mitochondrial-Anchored Protein Ligase (MAPL). We further characterized SP1 protein in respect to its membrane topology and ubiquitin E3 ligase activity. |
| first_indexed | 2024-12-17T05:24:33Z |
| format | Article |
| id | doaj.art-6ccb89b472294b69984ca27f3c497bdc |
| institution | Directory Open Access Journal |
| issn | 1942-0889 |
| language | English |
| last_indexed | 2024-12-17T05:24:33Z |
| publishDate | 2017-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Communicative & Integrative Biology |
| spelling | doaj.art-6ccb89b472294b69984ca27f3c497bdc2022-12-21T22:01:54ZengTaylor & Francis GroupCommunicative & Integrative Biology1942-08892017-07-0110410.1080/19420889.2017.13389911338991Sequence and biochemical analysis of Arabidopsis SP1 protein, a regulator of organelle biogenesisRonghui Pan0Jianping Hu1Michigan State UniversityMichigan State UniversityPeroxisomes, chloroplasts, and mitochondria are essential eukaryotic organelles that host a suite of metabolic processes crucial to energy metabolism and development. Regulatory mechanisms of the dynamics and biogenesis of these important organelles have begun to be discovered in plants. We recently showed that, aside from its previously reported role in targeting chloroplast protein import proteins, the Arabidopsis ubiquitin E3 ligase SP1 (suppressor of ppi1 locus1) negatively regulates peroxisome matrix protein import by promoting the ubiquitination and destabilization of PEX13 and possibly PEX14 and other components of the peroxisome protein import apparatus. Here, we compared protein sequence and domain structure of SP1-like proteins in Arabidopsis and their human homolog, Mitochondrial-Anchored Protein Ligase (MAPL). We further characterized SP1 protein in respect to its membrane topology and ubiquitin E3 ligase activity.http://dx.doi.org/10.1080/19420889.2017.1338991chloroplastsE3 ubiquitin ligaseMAPLmitochondriaperoxisomesSP1SPL1 |
| spellingShingle | Ronghui Pan Jianping Hu Sequence and biochemical analysis of Arabidopsis SP1 protein, a regulator of organelle biogenesis Communicative & Integrative Biology chloroplasts E3 ubiquitin ligase MAPL mitochondria peroxisomes SP1 SPL1 |
| title | Sequence and biochemical analysis of Arabidopsis SP1 protein, a regulator of organelle biogenesis |
| title_full | Sequence and biochemical analysis of Arabidopsis SP1 protein, a regulator of organelle biogenesis |
| title_fullStr | Sequence and biochemical analysis of Arabidopsis SP1 protein, a regulator of organelle biogenesis |
| title_full_unstemmed | Sequence and biochemical analysis of Arabidopsis SP1 protein, a regulator of organelle biogenesis |
| title_short | Sequence and biochemical analysis of Arabidopsis SP1 protein, a regulator of organelle biogenesis |
| title_sort | sequence and biochemical analysis of arabidopsis sp1 protein a regulator of organelle biogenesis |
| topic | chloroplasts E3 ubiquitin ligase MAPL mitochondria peroxisomes SP1 SPL1 |
| url | http://dx.doi.org/10.1080/19420889.2017.1338991 |
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