Differentially Expressed Genes Shared by Two Distinct Cytoplasmic Male Sterility (CMS) Types of <i>Silene vulgaris</i> Suggest the Importance of Oxidative Stress in Pollen Abortion
Cytoplasmic male sterility (CMS), encoded by the interacting mitochondrial and nuclear genes, causes pollen abortion or non-viability. CMS is widely used in agriculture and extensively studied in crops. Much less is known about CMS in wild species. We performed a comparative transcriptomic analysis...
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MDPI AG
2020-12-01
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| author | Manuela Krüger Oushadee A. J. Abeyawardana Claudia Krüger Miloslav Juříček Helena Štorchová |
| author_facet | Manuela Krüger Oushadee A. J. Abeyawardana Claudia Krüger Miloslav Juříček Helena Štorchová |
| author_sort | Manuela Krüger |
| collection | DOAJ |
| description | Cytoplasmic male sterility (CMS), encoded by the interacting mitochondrial and nuclear genes, causes pollen abortion or non-viability. CMS is widely used in agriculture and extensively studied in crops. Much less is known about CMS in wild species. We performed a comparative transcriptomic analysis of male sterile and fertile individuals of <i>Silene vulgaris</i>, a model plant for the study of gynodioecy, to reveal the genes responsible for pollen abortion in this species. We used RNA-seq datasets previously employed for the analysis of mitochondrial and plastid transcriptomes of female and hermaphrodite flower buds, making it possible to compare the transcriptomes derived from three genomes in the same RNA specimen. We assembled de novo transcriptomes for two haplotypes of <i>S. vulgaris</i> and identified differentially expressed genes between the females and hermaphrodites, associated with stress response or pollen development. The gene for alternative oxidase was downregulated in females. The genetic pathways controlling CMS in <i>S. vulgaris</i> are similar to those in crops. The high number of the differentially expressed nuclear genes contrasts with the uniformity of organellar transcriptomes across genders, which suggests these pathways are evolutionarily conserved and that selective mechanisms may shield organellar transcription against changes in the cytoplasmic transcriptome. |
| first_indexed | 2024-03-10T14:02:04Z |
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| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T14:02:04Z |
| publishDate | 2020-12-01 |
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| spelling | doaj.art-6cd1d00d646f40caad11872c7ced382a2023-11-21T01:02:30ZengMDPI AGCells2073-44092020-12-01912270010.3390/cells9122700Differentially Expressed Genes Shared by Two Distinct Cytoplasmic Male Sterility (CMS) Types of <i>Silene vulgaris</i> Suggest the Importance of Oxidative Stress in Pollen AbortionManuela Krüger0Oushadee A. J. Abeyawardana1Claudia Krüger2Miloslav Juříček3Helena Štorchová4Institute of Experimental Botany, Czech Academy of Sciences, Rozvojová 263, 16502 Prague, Czech RepublicInstitute of Experimental Botany, Czech Academy of Sciences, Rozvojová 263, 16502 Prague, Czech RepublicInstitute of Experimental Botany, Czech Academy of Sciences, Rozvojová 263, 16502 Prague, Czech RepublicInstitute of Experimental Botany, Czech Academy of Sciences, Rozvojová 263, 16502 Prague, Czech RepublicInstitute of Experimental Botany, Czech Academy of Sciences, Rozvojová 263, 16502 Prague, Czech RepublicCytoplasmic male sterility (CMS), encoded by the interacting mitochondrial and nuclear genes, causes pollen abortion or non-viability. CMS is widely used in agriculture and extensively studied in crops. Much less is known about CMS in wild species. We performed a comparative transcriptomic analysis of male sterile and fertile individuals of <i>Silene vulgaris</i>, a model plant for the study of gynodioecy, to reveal the genes responsible for pollen abortion in this species. We used RNA-seq datasets previously employed for the analysis of mitochondrial and plastid transcriptomes of female and hermaphrodite flower buds, making it possible to compare the transcriptomes derived from three genomes in the same RNA specimen. We assembled de novo transcriptomes for two haplotypes of <i>S. vulgaris</i> and identified differentially expressed genes between the females and hermaphrodites, associated with stress response or pollen development. The gene for alternative oxidase was downregulated in females. The genetic pathways controlling CMS in <i>S. vulgaris</i> are similar to those in crops. The high number of the differentially expressed nuclear genes contrasts with the uniformity of organellar transcriptomes across genders, which suggests these pathways are evolutionarily conserved and that selective mechanisms may shield organellar transcription against changes in the cytoplasmic transcriptome.https://www.mdpi.com/2073-4409/9/12/2700cytoplasmic male sterility<i>Silene vulgaris</i>differential gene expression |
| spellingShingle | Manuela Krüger Oushadee A. J. Abeyawardana Claudia Krüger Miloslav Juříček Helena Štorchová Differentially Expressed Genes Shared by Two Distinct Cytoplasmic Male Sterility (CMS) Types of <i>Silene vulgaris</i> Suggest the Importance of Oxidative Stress in Pollen Abortion Cells cytoplasmic male sterility <i>Silene vulgaris</i> differential gene expression |
| title | Differentially Expressed Genes Shared by Two Distinct Cytoplasmic Male Sterility (CMS) Types of <i>Silene vulgaris</i> Suggest the Importance of Oxidative Stress in Pollen Abortion |
| title_full | Differentially Expressed Genes Shared by Two Distinct Cytoplasmic Male Sterility (CMS) Types of <i>Silene vulgaris</i> Suggest the Importance of Oxidative Stress in Pollen Abortion |
| title_fullStr | Differentially Expressed Genes Shared by Two Distinct Cytoplasmic Male Sterility (CMS) Types of <i>Silene vulgaris</i> Suggest the Importance of Oxidative Stress in Pollen Abortion |
| title_full_unstemmed | Differentially Expressed Genes Shared by Two Distinct Cytoplasmic Male Sterility (CMS) Types of <i>Silene vulgaris</i> Suggest the Importance of Oxidative Stress in Pollen Abortion |
| title_short | Differentially Expressed Genes Shared by Two Distinct Cytoplasmic Male Sterility (CMS) Types of <i>Silene vulgaris</i> Suggest the Importance of Oxidative Stress in Pollen Abortion |
| title_sort | differentially expressed genes shared by two distinct cytoplasmic male sterility cms types of i silene vulgaris i suggest the importance of oxidative stress in pollen abortion |
| topic | cytoplasmic male sterility <i>Silene vulgaris</i> differential gene expression |
| url | https://www.mdpi.com/2073-4409/9/12/2700 |
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