Prevention of renal dysfunction by nutraceuticals prepared from oil rich plant foods
Objective: To investigate the protective effect of extracts prepared from avocado, walnut, flaxseed and Eruca sativa seeds in a rat model of kidney dysfunction induced by intraperitoneal cisplatin. Methods: Ethanol and petroleum ether extracts mixture was prepared from each plant. Six groups of rats...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-08-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115300770 |
| _version_ | 1819084251905130496 |
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| author | Sahar Y. Al-Okbi Doha A. Mohamed Thanaa E. Hamed Reham SH. Esmail Souria M. Donya |
| author_facet | Sahar Y. Al-Okbi Doha A. Mohamed Thanaa E. Hamed Reham SH. Esmail Souria M. Donya |
| author_sort | Sahar Y. Al-Okbi |
| collection | DOAJ |
| description | Objective: To investigate the protective effect of extracts prepared from avocado, walnut, flaxseed and Eruca sativa seeds in a rat model of kidney dysfunction induced by intraperitoneal cisplatin.
Methods: Ethanol and petroleum ether extracts mixture was prepared from each plant. Six groups of rats were conducted; control healthy, cisplatin group and four test groups where rats were given daily oral dose of each extract mixture before cisplatin injection. Different biochemical and cytogenetic parameters and kidney histopathology were determined. Acute toxicity was tested for the nutraceuticals. Total phenolic contents, fatty acids (FA) and unsaponifiable matter were assessed in the extracts.
Results: Walnut ethanol extract showed the highest content of total phenolic. FA analysis revealed that all the studied plants were rich in unsaturated FA. Gas-liquid chromatographic investigation of the unsaponifiable matter showed the presence of campesterol, stigmasterol and β-sitosterol in all the studied plants. Cisplatin treatment induced significant increase in plasma urea, creatinine and malondialdehyde along with significant reduction of plasma albumin, total protein, catalase and total antioxidant as well as reduction in creatinine clearance. Histopathological examination proved the induction of kidney dysfunction. Some sorts of chromosomal aberration and sperm-shape abnormalities were noticed after cisplatin treatment. Administration of extracts mixtures produced improvements in biochemical, histopathological and cytogenetic parameters.
Conclusions: Administration of the studied nutraceuticals proved to possess protective role against cisplatin-induced nephrotoxicity, chromosomal aberration and abnormal sperms. All studied nutraceuticals showed complete safety. |
| first_indexed | 2024-12-21T20:45:30Z |
| format | Article |
| id | doaj.art-6cd37ef261594a28856fbe3ac9531fe5 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-21T20:45:30Z |
| publishDate | 2014-08-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-6cd37ef261594a28856fbe3ac9531fe52022-12-21T18:50:50ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912014-08-014861862610.12980/APJTB.4.201414B66Prevention of renal dysfunction by nutraceuticals prepared from oil rich plant foodsSahar Y. Al-Okbi0Doha A. Mohamed1Thanaa E. Hamed2Reham SH. Esmail3Souria M. Donya4Food Sciences and Nutrition Department, National Research Centre, Dokki, Cairo, EgyptFood Sciences and Nutrition Department, National Research Centre, Dokki, Cairo, EgyptFood Sciences and Nutrition Department, National Research Centre, Dokki, Cairo, EgyptPathology Department, National Research Centre, Dokki, Cairo, EgyptCytogenetic Department, National Research Centre, Dokki, Cairo, EgyptObjective: To investigate the protective effect of extracts prepared from avocado, walnut, flaxseed and Eruca sativa seeds in a rat model of kidney dysfunction induced by intraperitoneal cisplatin. Methods: Ethanol and petroleum ether extracts mixture was prepared from each plant. Six groups of rats were conducted; control healthy, cisplatin group and four test groups where rats were given daily oral dose of each extract mixture before cisplatin injection. Different biochemical and cytogenetic parameters and kidney histopathology were determined. Acute toxicity was tested for the nutraceuticals. Total phenolic contents, fatty acids (FA) and unsaponifiable matter were assessed in the extracts. Results: Walnut ethanol extract showed the highest content of total phenolic. FA analysis revealed that all the studied plants were rich in unsaturated FA. Gas-liquid chromatographic investigation of the unsaponifiable matter showed the presence of campesterol, stigmasterol and β-sitosterol in all the studied plants. Cisplatin treatment induced significant increase in plasma urea, creatinine and malondialdehyde along with significant reduction of plasma albumin, total protein, catalase and total antioxidant as well as reduction in creatinine clearance. Histopathological examination proved the induction of kidney dysfunction. Some sorts of chromosomal aberration and sperm-shape abnormalities were noticed after cisplatin treatment. Administration of extracts mixtures produced improvements in biochemical, histopathological and cytogenetic parameters. Conclusions: Administration of the studied nutraceuticals proved to possess protective role against cisplatin-induced nephrotoxicity, chromosomal aberration and abnormal sperms. All studied nutraceuticals showed complete safety.http://www.sciencedirect.com/science/article/pii/S2221169115300770NutraceuticalCisplatinKidney dysfunctionRatsOxidative stress |
| spellingShingle | Sahar Y. Al-Okbi Doha A. Mohamed Thanaa E. Hamed Reham SH. Esmail Souria M. Donya Prevention of renal dysfunction by nutraceuticals prepared from oil rich plant foods Asian Pacific Journal of Tropical Biomedicine Nutraceutical Cisplatin Kidney dysfunction Rats Oxidative stress |
| title | Prevention of renal dysfunction by nutraceuticals prepared from oil rich plant foods |
| title_full | Prevention of renal dysfunction by nutraceuticals prepared from oil rich plant foods |
| title_fullStr | Prevention of renal dysfunction by nutraceuticals prepared from oil rich plant foods |
| title_full_unstemmed | Prevention of renal dysfunction by nutraceuticals prepared from oil rich plant foods |
| title_short | Prevention of renal dysfunction by nutraceuticals prepared from oil rich plant foods |
| title_sort | prevention of renal dysfunction by nutraceuticals prepared from oil rich plant foods |
| topic | Nutraceutical Cisplatin Kidney dysfunction Rats Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115300770 |
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